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Indigofera tinctoria, also called true indigo, is a species of plant from the bean family that was one of the original sources of indigo dye. It has been naturalized to tropical and temperate Asia, as well as parts of Africa, but its native habitat is unknown since it has been in cultivation worldwide for many centuries. Today most dye is synthetic, but natural dye from I. tinctoria is still available, marketed as natural coloring where it is known as tarum in Indonesia and nila in Malaysia. In Iran and areas of the former Soviet Union it is known as basma. The plant is also widely grown as a soil-improving groundcover.
Method for preparing multifunctional indigo and polyphenol extract from malan stem and leaf
Using the stems and leaves of *Indigofera tinctoria* as raw material, a combined high-pressure water extraction technology involving water spray washing and grading, along with steam explosion, was employed to effectively improve the release rate of active substances such as indole glycosides and polyphenols, as well as the conversion rate of indigo. Multifunctional indigo and polyphenol extracts were prepared through bio-fermentation and membrane fractionation purification. The indigo extract contained significantly more bioactive substances than traditional lime-based indigo powder and indigo acidified compounds, with 15 times more indigo, 25 times less ash, and 650 times less calcium. Compared to natural Indian indigo, it exhibited superior dyeing performance on cotton fabrics and showed better inhibitory effects against *Aspergillus niger*, *Staphylococcus aureus*, and *Escherichia coli*. The *Indigofera tinctoria* polyphenol extract, composed of phenolic acids, phenylethyl glycosides, flavonoids, and polysaccharides, demonstrated excellent inhibitory activity against *Staphylococcus aureus* (IC50 = 32 μg / mL) and a wound healing rate exceeding 98% in mice, exhibiting good broad-spectrum antibacterial and wound-healing properties.
Owner:INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY