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The invention belongs to the field of pharmaceutical preparations, and particularly relates to a cetrorelix acetate preparation composition for injection and a preparation method thereof.According to the preparation composition, through a specific freeze-drying process including a slow freezing stage, an annealing stage, primary drying and secondary drying, it is effectively guaranteed that the in-batch quality uniformity of a preparation is good, and the long-term stability is excellent; and industrial mass production is facilitated.