Micro-capillary electropheresis apparatus

A capillary electrophoresis and capillary technology, which is applied in the field of capillary electrophoresis and microscopic imaging, can solve the problems of electrophoresis pattern and the morphology of the measured object, inability to achieve efficient, high-speed and high-automatic operation, and inability to analyze.

Inactive Publication Date: 2008-06-11
INST OF CHEM CHINESE ACAD OF SCI
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Problems solved by technology

At present, the detection methods of capillary electrophoresis, whether it is ultraviolet (UV), laser-induced fluorescence (LIF), or diode array (DAD) or other means, cannot enable the experimenter to obtain the electrophoretic pattern and the morphology of the measured object at the same time. Overlapping peaks can only be separated by changing various conditions regardless of the analyte, otherwise it cannot be analyzed; traditional microelectrophoresis can observe the morphology characteristics of particle samples, but it cannot achieve the high efficiency, high speed and high efficiency of capillary electrophoresis. Highly automated operation, also does not have spectral analysis function, so there are major defects in application

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Effect test

Embodiment 1

[0023] see figure 1 and figure 2 , the concrete structure of micro capillary electrophoresis instrument of the present invention is:

[0024] The two liquid bottles 4 are connected by a capillary 2 to form a capillary channel, and the two liquid bottles are respectively connected to the high-voltage power supply 1 through the electrodes 3 to form an electrophoresis system. The capillary 2 is made of transparent material, and its diameter and shape are not limited, and can be selected and replaced as required. The high-voltage power supply is a known DC power supply, which can output voltages and currents of different sizes.

[0025] An electrophoresis spectrum collection system and an image collection system are respectively arranged around the capillary 2 . Wherein the composition of the electrophoretic spectrum acquisition system is: a light source 5 is arranged on one side of the capillary 2, and a photoelectric converter 8 is arranged on the other side of the capillary...

Embodiment 2

[0031] Using the micro capillary electrophoresis instrument of embodiment 1, the quartz capillary (75 μm inner diameter) is passed through CH 3 OH, H 2 O, NaOH, H 2 O was pressure washed in turn, and pressure injected into 0.15mmol / LTris-H 3 BO 3 , after stabilization, the differential pressure enters the rabbit's red blood cells. Use UV detection to collect the electrophoresis spectrum at 214nm, and you can get the UV absorption peak of the cells image 3 ; Use an inverted microscope with CCD and computer to collect images as shown in Figure 4 (A is the image of a single dispersed cell, B is the image of a cell aggregate), and the movement process of the cells can be seen.

Embodiment 3

[0033] Use the micro capillary electrophoresis apparatus of embodiment 1, after cleaning capillary with embodiment 1 with the same method, pressure injects 0.15mmol / L Tris-H 3 BO 3 , after stabilization, the differential pressure enters the person's red blood cells. Use UV detection to collect the electrophoresis spectrum at 214nm, and you can get the UV absorption peak of the cells Figure 5 ; Use an inverted microscope with a CCD and a computer to collect images as shown in Figure 6 (A is an image of a single dispersed cell, B is an image of a cell aggregate and a single cell), and the movement process of the cells can be seen.

[0034] As can be seen from the above examples, the present invention combines capillary electrophoresis and microscopic imaging technology, and has the following characteristics:

[0035]It can realize high-speed electrophoresis of microscopic images, single-cell or single-particle analysis, simultaneous rapid analysis of homogeneous and non-homog...

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Abstract

The present invention relates to a microscope capillary electrophoresis apparatus. It is mainly formed from capillary electrophoresis system, electrophoresis pattern collection system and image collection system, in which the capillary electrophoresis system includes two liquid bottles, capillary tube and high-voltage power supply, the electrophoresis pattern collection system includes light source and photoelectric converter, and the image collection system includes a microscope light source, a microscope, an image collector and an image display processor.

Description

technical field [0001] The invention relates to two technologies of capillary electrophoresis and microscopic imaging, and belongs to a new technology for combining capillary electrophoresis and microscopic imaging. Background technique [0002] Capillary electrophoresis is a new separation technology in the past 20 years, while microscopic imaging is a relatively mature observation technology, but the combination of the two is an innovative technology invention. Capillary electrophoresis is one of the current research hotspots, and microscopy is one of the important means of biological and other scientific research. At present, the detection methods of capillary electrophoresis, whether it is ultraviolet (UV), laser-induced fluorescence (LIF), or diode array (DAD) or other means, cannot enable the experimenter to obtain the electrophoretic pattern and the morphology of the measured object at the same time. Overlapping peaks can only be separated by changing various conditi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447G01N21/84
Inventor 陈义戴东升齐莉余晓
Owner INST OF CHEM CHINESE ACAD OF SCI
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