Solid composite microbe microsphere for soil rehabilitation and its preparation method

A technology for composite microorganisms and soil remediation, which is applied in the field of solid composite microbial microspheres for soil remediation and its preparation, and can solve the problems of inapplicability of macromolecular substrates, hindrance of substrate and oxygen diffusion, etc.

Active Publication Date: 2008-09-10
CHINA PETROLEUM & CHEM CORP +1
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Problems solved by technology

On the other hand, in the process of cell immobilization application, since the embedding material hinders the substrate and oxygen diffusion to a certain extent, it is especially not suitable for macromolecular substrates

Method used

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  • Solid composite microbe microsphere for soil rehabilitation and its preparation method
  • Solid composite microbe microsphere for soil rehabilitation and its preparation method

Examples

Experimental program
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Embodiment 1

[0023] 1) Plane culture: adopt Yinhan bacteria and Pseudomonas as embedded fungi and bacteria. Potato plane agar (PDA) and LB medium were prepared by microbiological methods, and C. chinensis was inoculated on the prepared PDA plane medium, cultured at 28°C for 4 days, and stored for later use; Pseudomonas Inoculate onto the prepared LB plane medium, culture at 30°C for 2 days, and save for later use;

[0024] 2) Shake flask culture: prepare PDA liquid medium 100ml and LB liquid medium 100ml according to the microbiological method, put into the Erlenmeyer flask that volume is 300ml respectively, sterilize under high temperature at 121 ℃ for 15 minutes, cool to room temperature, then in Inoculate silverella thorns and Pseudomonas in a sterile workbench respectively, and place the shaker flask in an air shaker. The fungal culture condition is 28°C, 160rpm, and cultured for 4 days; the bacterial culture condition is 30°C, 160rpm culture 1 day; collect the bacterial liquid, and m...

Embodiment 2

[0030] Embodiment 2 differs from Embodiment 1 in that:

[0031] 1) Plane culture: use Yinhan bacteria thorns, Phanerochaete chrysosporium and Rhodococcus as embedded fungi and bacteria. Prepare potato plane medium (PDA) and LB medium by microbiological methods, inoculate the fungus on the prepared PDA plane medium, cultivate it at 25°C for 5 days, and save it for later use; inoculate the bacteria into the prepared LB plane culture cultured at 30°C for 2 days and stored for future use.

[0032] 2) Shake flask culture: Prepare 200ml of PDA liquid medium and 100ml of LB liquid medium according to microbiological methods, put them into a 300ml Erlenmeyer flask respectively, sterilize at 121°C for 15 minutes, cool to room temperature, and then Inoculate Silverhamella thorns, Phanerochaete chrysosporium, and Rhodococcus in a sterile workbench respectively, place the shaker flask in an air shaker, and culture the fungi at 25°C and 160 rpm for 5 days; the bacteria culture conditions ...

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Abstract

The present invention provides one kind of soil repairing solid compound microbe microball and its preparation process. The soil repairing solid compound microbe microball is prepared with calcium alginate as embedding material and solid calcium carbonate as solid pore-creating agent to embed petroleum hydrocarbon degrading fungi, bacteria and nutritive matter, and the preparation process includes suspension preparation, microball formation, eliminating pore-creating agent and other steps. The present invention has the advantages of solid carrier maintained, has super large pores for reinforced mass transfer and embedded fungi, bacteria and nutritive matter, and thus possesses the advantages of high mass transferring speed, protection, nourishing, high activity and stability, etc. This kind of solid compound microbe microball may find its wide application in the biological repair of petroleum polluted soil.

Description

technical field [0001] The invention relates to a solid composite microbial microsphere for soil restoration and a preparation method thereof, which belongs to the preparation technology of microbial preparations for bioremediation of petroleum-contaminated soil. Background technique [0002] Environmental bioremediation technology is mainly composed of three aspects: (1) The technology of utilizing the metabolic ability of indigenous microorganisms (Natural Attenuation); (2) The method of activating the decomposition ability of indigenous microorganisms (Biostimulation, referred to as biological activation method): (3) Adding A method of specific microorganisms (groups) with the ability to decompose refractory compounds at a high speed (Bioaugmentation, referred to as biological addition method). For the bioremediation process of petroleum-contaminated soil, due to the complex composition, biodegradability and low bioavailability of petroleum pollutants, it is difficult for...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/10C12S13/00C12P1/02C12P1/04C12S99/00
Inventor 刘铮韩慧龙周鑫闫明张敏莲王福远张坤
Owner CHINA PETROLEUM & CHEM CORP
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