Oxygen-enriched catalyst large scale production method of plant erythrocuprein

Abstract

The invention discloses a plant superoxide dismutase oxygen enrichment catalysis large scale producing method. It includes the following steps: selecting plant seed; removing impurity; sterilizing; oxygen enrichment pre-catalyzing; catalyzing; homogenization treatment and control pH at 7-8; dehydrating and collecting feed liquid; filtering and drying. Its advantages are that it can improve superoxide dismutase yield, increase its recovery by 30-50% compared with the existing method; it has simple technology, low cost, and wide material adaptability; it can used in large scale production.

Description

(1) Technical field:

[0001] The invention relates to a production method of plant superoxide dismutase, in particular to a large-scale production method of superoxide dismutase with oxygen-enriched catalysis. (two) background technology:

[0002] Superoxide dismutase (SOD: Superoxide Dismutase) is a biological catalyst for oxygen free radicals (O 2 - ·) An important enzyme for disproportionation reactions, with a molecular weight of about 34,000 Daltons. Its catalytic speed to superoxide is 10,000 times that of chemical energy, and it can eliminate the poison of oxygen free radicals to the human body. It is internationally recognized as a scavenger of oxygen free radicals. Since McCord and Fridovich first discovered superoxide disproportionation from bovine blood in 1969, it has attracted the attention of biochemical and medical circles in various countries. Since the 1990s, the scientific community has realized that oxygen free radicals are excess free radicals produced ...

Examples

Embodiment 1

[0015] Remove impurities from 1 ton of air-dried corn grains with a drum sieve and a magnetic separator, soak them in an aqueous solution with an ozone content of 2 mg / L for 60 minutes for disinfection and sterilization, and then rinse them with clean water;

[0016] Put the raw material into a closed environment with an oxygen content of 70% (weight percentage), spray it with circulating water at 25°C for 48 hours to carry out pre-catalysis, make it absorb water and expand, and start to carry out oxygen-enriched catalysis after pre-catalysis. Putting it into an airtight environment with an oxygen content of 70% (percentage by weight), spraying it with circulating water at 30°C for 60 hours to germinate;

[0017] The raw materials after oxygen-enriched catalysis are homogenized on the existing large-scale homogenizer, the homogenization pressure is controlled to 20Mpa, and the output particle size is ≤1μm. Phosphate buffer solution is added during the homogenization process to ...

Embodiment 2

[0019] Embodiment 2: the material liquid A among the embodiment 1 is filtered with existing micron filter, molecular weight cut-off is 50,000, collects material liquid B, and material liquid B can be made into middle content superoxide dismutase through freeze-drying The product is 825g, and the content of superoxide dismutase is 177.2U / mg through the detection of pyrogallol autoxidation method, and the yield of superoxide dismutase is 146 million U / ton of raw corn.

Embodiment 3

[0020] Embodiment 3: the feed liquid B among the embodiment 2 is filtered with the existing nano-scale filter, the molecular weight cut-off is about 10,000, and the feed liquid C is collected, and the feed liquid C can be made into high-content superoxide disproportionation through freeze-drying 105g of the enzyme product, detected by the pyrogallol autoxidation method, the superoxide dismutase content is 1320U / mg, and the superoxide dismutase yield is 139 million U / ton raw corn.

(1) Technical field:

[0001] The invention relates to a production method of plant superoxide dismutase, in particular to a large-scale production method of superoxide dismutase with oxygen-enriched catalysis. (two) background technology:

[0002] Superoxide dismutase (SOD: Superoxide Dismutase) is a biological catalyst for oxygen free radicals (O 2 - ·) An important enzyme for disproportionation reactions, with a molecular weight of about 34,000 Daltons. Its catalytic speed to superoxide is 10,000 times that of chemical energy, and it can eliminate the poison of oxygen free radicals to the human body. It is internationally recognized as a scavenger of oxygen free radicals. Since McCord and Fridovich first discovered superoxide disproportionation from bovine blood in 1969, it has attracted the attention of biochemical and medical circles in various countries. Since the 1990s, the scientific community has realized that oxygen free radicals are excess free radicals produced ...

Examples

Embodiment 1

[0015] Remove impurities from 1 ton of air-dried corn grains with a drum sieve and a magnetic separator, soak them in an aqueous solution with an ozone content of 2 mg / L for 60 minutes for disinfection and sterilization, and then rinse them with clean water;

[0016] Put the raw material into a closed environment with an oxygen content of 70% (weight percentage), spray it with circulating water at 25°C for 48 hours to carry out pre-catalysis, make it absorb water and expand, and start to carry out oxygen-enriched catalysis after pre-catalysis. Putting it into an airtight environment with an oxygen content of 70% (percentage by weight), spraying it with circulating water at 30°C for 60 hours to germinate;

[0017] The raw materials after oxygen-enriched catalysis are homogenized on the existing large-scale homogenizer, the homogenization pressure is controlled to 20Mpa, and the output particle size is ≤1μm. Phosphate buffer solution is added during the homogenization process to ...

Embodiment 2

[0019] Embodiment 2: the material liquid A among the embodiment 1 is filtered with existing micron filter, molecular weight cut-off is 50,000, collects material liquid B, and material liquid B can be made into middle content superoxide dismutase through freeze-drying The product is 825g, and the content of superoxide dismutase is 177.2U / mg through the detection of pyrogallol autoxidation method, and the yield of superoxide dismutase is 146 million U / ton of raw corn.

Embodiment 3

[0020] Embodiment 3: the feed liquid B among the embodiment 2 is filtered with the existing nano-scale filter, the molecular weight cut-off is about 10,000, and the feed liquid C is collected, and the feed liquid C can be made into high-content superoxide disproportionation through freeze-drying 105g of the enzyme product, detected by the pyrogallol autoxidation method, the superoxide dismutase content is 1320U / mg, and the superoxide dismutase yield is 139 million U / ton raw corn.