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A method for determining brown strip resistant rice species

A technology for leaf blight resistance and rice, which is applied in the field of identification of rice varieties resistant to stripe leaf blight, can solve the problems of delaying disease-resistant breeding, increasing the workload of disease-resistant variety breeding, and identifying dead seedlings, etc. Achieve the effect of improving accuracy, speeding up the process and reducing the workload

Inactive Publication Date: 2008-10-15
JIANGSU ACAD OF AGRI SCI
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Problems solved by technology

Although there are many identification methods used in the process of disease-resistant breeding, most of them use the method of inoculation identification at the seedling stage. Due to the particularity of the damage mode of rice stripe virus, the results often lead to the death of identified plants with poor resistance. Therefore, breeders often infer the resistance of contemporary materials by identifying the resistance of the second generation, which not only delays the work process of disease-resistant breeding, but also increases the workload of disease-resistant variety breeding

Method used

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  • A method for determining brown strip resistant rice species
  • A method for determining brown strip resistant rice species
  • A method for determining brown strip resistant rice species

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1, Inoculation Identification of Wuyujing No. 3 and Zhendao 88

[0020] Test inoculum:

[0021] according to figure 1 The high-affinity SBPH population Q population obtained by screening the rice stripe virus obtained by the method shown was detected by the RSV dot immunoassay with a virus-carrying rate of 73%.

[0022] Test plant material:

[0023] The rice stripe blight susceptible variety Wuyujing No. 3 was provided by the Wujin Rice and Wheat Breeding Farm in Jiangsu Province, and the rice stripe blight-resistant variety Zhendao 88 was provided by the Hilly Agricultural Science Institute of the Jiangsu Academy of Agricultural Sciences. kind.

[0024] The resistance identification method is as follows:

[0025] 1. Culture and preparation of RSV inoculum

[0026] The Q population of SBPH was cultivated in a light-temperature cultivation room, and the temperature was controlled at 15-25°C. figure 2 The breeding method is shown, and when the striatellus g...

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Abstract

The invention relates to a method for checking the resistance of rice specy toward to leaf blight, wherein it based on the character of tilering plant to remove the tiler, to check the resistance at controllable condition; and based on the resistance appearance of tiler, evaluates the resistance of whole plant. The invention has high accuracy and wide check time.

Description

technical field [0001] The invention is a new method for identifying the resistance of rice varieties to rice stripe blight, which belongs to the identification technology of plant disease resistance. Background technique [0002] Rice stripe blight caused by rice stripe virus (RSV) was first discovered in Kanto, Japan in 1897, and later occurred in Korea, Ukraine and China. Since the virus was discovered in Jiangsu and Zhejiang in 1962 in my country, it has occurred in 16 provinces, municipalities, and autonomous regions across the country. At present, the rice fields in the lower reaches of the Yangtze River, mainly in Jiangsu Province, are still in the epidemic period. According to incomplete statistics, the occurrence area of ​​rice stripe blight in Jiangsu Province reached 27 million mu in 2004 alone, accounting for 79% of the rice area in Jiangsu Province. Experts predict that rice stripe blight will still show a tendency to occur more heavily in the five years after 2...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G16/00A01G7/00A01G22/22
Inventor 周彤周益军程兆榜
Owner JIANGSU ACAD OF AGRI SCI
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