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Cryptoporus volvatus polysaccharide, preparation and application thereof

A technology of cryptoporous bacteria and polysaccharides, applied in the field of cryptoporous bacteria polysaccharides and its preparation, can solve the problems of few researches on active ingredients of cryptoporous bacteria, and achieve the effect of low cost, high content and high yield

Inactive Publication Date: 2009-07-08
杭州赐富生物技术有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, little research has been done on the active ingredients of Cryptoporus

Method used

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  • Cryptoporus volvatus polysaccharide, preparation and application thereof
  • Cryptoporus volvatus polysaccharide, preparation and application thereof
  • Cryptoporus volvatus polysaccharide, preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: the preparation of cryptoporus polysaccharide

[0033] Take 2000 grams of Cryptoporus bacteria powder, add 10 liters of water, and extract at 80°C for 2 hours. The extract was centrifuged at 3500 rpm for 10 minutes to obtain supernatant 1. Add 2 liters of water to the precipitate, fully stir and extract, and centrifuge at 3500 rpm for 10 minutes to obtain supernatant 2. Combine supernatant 1 and supernatant 2, concentrate under reduced pressure to about 6 liters, add 1000 g of PEG6000 under constant stirring, and continue stirring for 1 hour to fully dissolve PEG. Place at room temperature overnight to make the precipitation complete, then centrifuge the solution at 3500 rpm for 10 minutes to obtain a precipitate, and discard the supernatant. The precipitate was reflux extracted with 95% ethanol in a Soxhlet extractor for 6 hours until the extract was basically colorless, the ethanol in the precipitate was evaporated and weighed to obtain 82 grams of pre...

Embodiment 2

[0034] Embodiment 2: the preparation of cryptoporus polysaccharide

[0035] Take 200 grams of natural Cryptoporus, crush it, add 500 milliliters of water, and extract at 80°C for 2 hours. The extract was centrifuged at 3500 rpm for 10 minutes to obtain supernatant 1. Add 200 ml of water to the precipitate, fully stir and extract, and centrifuge at 3500 rpm for 10 minutes to obtain supernatant 2. Combine supernatant 1 and supernatant 2, concentrate under reduced pressure to about 200 ml, add 50 g of PEG20000 under constant stirring, and continue stirring for 1 hour to fully dissolve PEG. Place at room temperature overnight to make the precipitation complete, then centrifuge the solution at 3500 rpm for 10 minutes to obtain a precipitate, and discard the supernatant. The precipitate was reflux extracted with 95% ethanol in a Soxhlet extractor for 6 hours until the extract was basically colorless, the ethanol in the precipitate was evaporated and weighed to obtain 4.2 g of prec...

Embodiment 3

[0036] Embodiment 3: the preparation of cryptoporus polysaccharide

[0037] Take 2000 grams of Cryptoporus mycelium, add 10 liters of water, and extract at 80° C. for 2 hours. The extract was centrifuged at 3500 rpm for 10 minutes to obtain supernatant 1. Add 2 liters of water to the precipitate, fully stir and extract, and centrifuge at 3500 rpm for 10 minutes to obtain supernatant 2. Combine supernatant 1 and supernatant 2, concentrate under reduced pressure to about 1 liter, add 200 g of PEG6000 under constant stirring, and continue stirring for 1 hour to fully dissolve PEG. Place at room temperature overnight to make the precipitation complete, then centrifuge the solution at 3500 rpm for 10 minutes to obtain a precipitate, and discard the supernatant. The precipitate was extracted with 95% ethanol under reflux for 6 hours, and the extracted precipitate was dried and weighed to obtain 8.2 g of precipitate. Dissolve the precipitate with 500 ml of water, centrifuge at 350...

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Abstract

The invention provides a cryptoporous polysaccharide and a preparation method thereof, as well as the application of the cryptoporous polysaccharide in the preparation of medicines for treating allergic rhinitis and allergic asthma, and its application in the preparation of medicines for treating adult respiratory distress syndrome. application. The beneficial effects of the cryptoporous polysaccharide of the present invention and its preparation and application are mainly reflected in: (1) the medicinal composition of the cryptoporous polysaccharide of the present invention is clearer, and can significantly inhibit the antigenic attack of sensitized rats The effect of pulmonary eosinophilic inflammation can be developed into a new drug or functional food or health product for allergic rhinitis and allergic asthma, which has broad application prospects; (2) the preparation method of the cryptoporous polysaccharide uses PEG precipitation The polysaccharide content of the obtained product is much higher than that obtained by ethanol precipitation, and the amount of PEG is only about one-twentieth of that of ethanol, and the yield is high, the content is high, the cost is low, and the operation is simple.

Description

(1) Technical field [0001] The invention relates to a cryptoporous polysaccharide and a preparation method thereof, as well as the application of the cryptoporous polysaccharide in preparing medicines for treating allergic rhinitis, allergic asthma and adult respiratory distress syndrome. (2) Background technology [0002] The common name of cryptoporia is pine olive, tree lotus bud, tree pimple, chrysanthemum fungus, wood fish fungus, fragrant magnolia, black maizi (Yunnan), and a mouthful of velvet (Japan). Belongs to the kingdom Fungi, Basidiomycotina, Hymenomycetes, Aphyllophorales, Polyporales in the old system, Polyporaceae, Cryptoporus . Distributed in Sichuan, Jiangsu, Zhejiang, Hubei, Jilin, Yunnan, Hainan, Fujian and other provinces in China, and also in Korea, Japan, Europe, and North America. [0003] There have been reports on the medicinal value of Cryptoporus bacterium for a long time. Lan Mao (1397-1476) "Southern Yunnan Materia Medica" records: bitter, swe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C12P19/04A61K31/715A61P11/06
Inventor 柯传奎杨勇杰谢强敏陈黎欧阳小军柴黎燕
Owner 杭州赐富生物技术有限公司
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