Serum sodium ion enzyme method determination agent and method

Abstract

The invention discloses a reagent and a method for the enzymatic determination of sodium ions in serum. The invention adopts crown ether, which has the characteristics of complexing between cryptate and sodium ions, and uses the ratio of different reagents to find a better concentration. In terms of sensitivity, accuracy, In terms of precision, it has the same effect as using cryptane. The measuring effect of the present invention is similar to that of cryptane, but the measuring method is simple and the cost is greatly reduced.

Description

Technical field [0001] The invention belongs to a clinical biochemical analysis method and is used for the determination of sodium ion in serum. Background technique [0002] In clinical trials of biochemistry, the determination of serum sodium ion is a routine analysis item in the hospital. The normal value of sodium is 136-145mmol / L, and the common clinical electrolyte disorder is hyponatremia. [0003] The earliest analytical method of sodium ion was flame photometry. The principle of this method is that some sodium atoms to be measured are excited by heating stimulation and emit light with characteristic wavelengths when they return to the ground state. The characteristic wavelength generated by the excited sodium atoms to be measured in the flame, the intensity of radiant energy is directly proportional to the number of excited sodium atoms to be measured in the flame, and the number of excited sodium atoms to be measured is directly proportional to the sample to be measured...

AI Technical Summary

Problems solved by technology

The original electrode method not only needs to purchase additional instruments, but also needs to replace the electrodes frequently, and the consumption of electrolyte is relatively fast. These reasons make the electrode method to determine the sodium ion in serum more costly, but the enzymatic method does not have these problems , so the measurement cost is much lower

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