Dimeric flavone compound as well as preparation and uses thereof
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A compound, technology of ephedra, applied in the field of dimeric flavonoid compounds
Active Publication Date: 2009-12-16
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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[0003] Ephedra contains alkaloids, flavonoids, flavans, tannins, volatile oils, organic acids and polysaccharides, etc., but currently only alkaloids are clearly studied on their pharmacological and toxicological effects
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Embodiment 1
[0014] Embodiment 1: the preparation of the dimeric flavonoid compound represented by formula (1)
[0015] Get 20 kg of ephedra roots, extract 3 times with 10 liters of ethanol under reflux, suspend 1000 g of ethanol extract with 5 liters of water, add 5 liters of petroleum ether to degrease, then separate liquid with 3 liters of ethyl acetate to obtain 200 grams of extract, 100 g of the extract was subjected to normal-phase silica gel flash chromatography (500 grams of silica gel in column loading, 10 cm in column diameter, 40 μm in silica gel particle size, and natural flow rate), and eluted with a gradient of chloroform-methanol at a volume ratio of 100:0 to 0:100. The volume ratio of 100:10 part of the eluate was combined to obtain 7.8 grams, and then eluted with 5 liters of chloroform-methanol with a volume ratio of 100:8, and the part with a ratio shift value of 0.35 was collected to obtain 330 mg, and then used Sephadex LH- 20, eluting and separating with chloroform-met...
Embodiment 2
[0022] Example 2: In vitro anti-tumor experimental research on the dimeric flavonoid compound obtained in Example 1
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Abstract
The invention relates to a novel demeric flavonoid compound expressed by the formula (1) and also relates to a preparation method for demeric flavonoid compound and the application of the demeric flavonoid compound in treatment of cancers, especially the cervical cancer, gastric cancer and liver cancer. The compound of the invention is obtained through separation from the roots of the Ephedra sinica. The experiments prove that the compound of the invention has intensive cytotoxicity and exerts an inhibitory action on the HeLa cervical carcinoma cells, SGC-7901 gastric cancer cells and HepG2 hepatocellular carcinoma cells. The IC50 values are all approximately 10Mug / mL, which indicates that the compound of the invention has more distinc growth inhibitory effect on the cancer cells.
Description
technical field [0001] The invention relates to a dimeric flavonoid compound, specifically a dimeric flavanol, a preparation method of the compound and its application in inhibiting the growth of cancer cells. Background technique [0002] Ephedra was first recorded in "Shen Nong's Materia Medica", and it was listed as a middle-grade product. It has been recorded in successive dynasties of herbal medicines. Bronchial asthma and other diseases. [0003] Ephedra contains alkaloids, flavonoids, flavans, tannins, volatile oils, organic acids and polysaccharides, etc., but currently only alkaloids are clearly studied on their pharmacological and toxicological effects. [0004] Domestic research on ephedrone is less, and ephedrine mainly includes: apigenin, wheat glutenin, kaempferol, apigenin 252 rhamnoside, gossythin, 32 methylamino gossin, kaempferol rhamnoside, Colorless delphinidin, rutin, pharyngein, white anthocyanins, colorless cyanidin and quercetin, etc. [0005] Ephe...
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Patent Type & Authority Patents(China)
IPC IPC(8): C07D493/18A61K31/357A61P35/00
Inventor 刘永宏陶华明王隶书
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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