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Sea hedgehog extractive, preparation method and anticancer application thereof

A technology of extract and sea urchin, applied in the field of sea urchin extract

Active Publication Date: 2010-05-19
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No anti-cancer extracts have been found from sea urchins

Method used

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  • Sea hedgehog extractive, preparation method and anticancer application thereof
  • Sea hedgehog extractive, preparation method and anticancer application thereof
  • Sea hedgehog extractive, preparation method and anticancer application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0014] Embodiment 1: the preparation of sea urchin extract of the present invention

[0015] Take Tripneustes gratilla with a wet weight of 4 kg, extract 3 times with 10 liters of methanol at room temperature, suspend 80 grams of methanol extract in 5 liters of water, add 5 liters of chloroform to separate the liquid, and condense part of the chloroform to obtain an extract 30 gram, and then use 3 liters of methanol with a volume fraction of 90% and 3 liters of n-hexane to separate liquids to obtain 12 grams of 90% methanol extract and 13 grams of n-hexane extract. (600 grams of reversed-phase silica gel, column height 10cm, column diameter 10cm, flow rate 20mL / min), elute with 5 liters of methanol-water with a volume ratio of 80:10, combine to obtain 4.4 grams, and use ODS reverse-phase silica gel flash chromatography (200 grams of reversed-phase silica gel, column height 10cm, column diameter 5cm, flow rate 10mL / min), separated, eluted with 1 liter of methanol-water with a v...

Embodiment 2

[0020] Example 2: In vitro anti-tumor experimental research on the sea urchin extract obtained in Example 1

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Abstract

The invention relates to an extractive of a sea urchin. The invention has the advantage that the invention consists essentially of a compound with 5 alpha and 8 alpha -two carbon radical in the following form (1) and is obtained from the seperation of the Tripneustes gratilla. The invention also relates to the preparation method of the extractive and the application on the cancer treatment. Provedby experiment, the extractive of the invention is provided with medium cytotoxicity, which can inhibit the producing of the HeLa cervical cancer cells, SGC-7901 gastric cancer cell, HepG2 liver cancer cell strain. The IC50 value ranges from 60 microgram per millilitre to 90 microgram per millilitre. The invention has comparatively inhibitory effect to the cancer cell is showed; the IC50 value ofthe noermal cell LO2 is 215 microgram per millilitre, which showes the extractive of the invention has antineoplastic activity within the safe dose.

Description

technical field [0001] The invention relates to a sea urchin extract, a preparation method of the extract and its application in inhibiting the growth of cancer cells. Background technique [0002] Sea urchins were first recorded in "Materia Medica", and they are animals of the class Echinoidea of ​​the phylum Echinodermata. [0003] The nature of sea urchin is "salty", "flat" ("Chinese Medicine Chronicle"), "slightly poisonous" ("Shandong Chinese Herbal Medicine Handbook"), "indications for distressed" ("Materia Medica Original"), and "soft and firm, resolving phlegm and swelling , eliminate scrofula, sputum core, accumulated phlegm, chest pain and other syndromes" effect, can be used to treat lymphatic tuberculosis and other diseases. Modern medicine has proved that its bone shell, spines, gonads and other parts have good medicinal value and are the main resources for the development of marine drugs. [0004] The basic structure of a sea urchin consists of spines, a bony...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/56A61P35/00C07D319/02A61K35/616
Inventor 刘永宏阎红任珅徐暾海
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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