Reagent kit for detecting lung cancer susceptibility by XRCC1 gene

A kit and susceptibility technology, applied in the field of kits for detecting susceptibility to lung cancer, can solve problems such as unclear etiology and pathogenesis

Inactive Publication Date: 2008-02-27
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Etiology and pathogenesis have not yet been elucidated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1. Use of detection kits

[0024] Step 1: Extraction of DNA template

[0025] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0026] Step 2: Real-time quantitative PCR reaction

[0027] Use a fluorescent quantitative PCR detection kit that can detect susceptibility to lung cancer, which contains the following primer pairs and fluorescent probes:

[0028] Sense primer: 5'-TAACTGGCATCTTCACTTCT-3' (SEQ ID NO: 2) with a Tm value of 56°C

[0029] Antisense primer: 5'-TCCAGATTCCTGGCATTGC-3' (SEQ ID NO: 3) with a Tm value of 58°C

[0030] Fluorescent probe: 5'-CGGCTGCCCTCCCAGAGG-3' (SEQ ID NO: 4) with a Tm value of 64°C

[0031] The fluorescent quantitative PCR reaction system has a total volume of 10 μl, including 2 μl of DNA template with a concentration of 20 ng / μl, 1 μl of 10X fluorescent quantitative PCR reaction buffer, 0.1 μl of 25mM dNTP mixture, and 0.6 μl of 25mM MgCl 2 solution, 0.025 μl (5 units / μl) Taq DNA polymerase, 0....

Embodiment 2

[0035] Example 2. Services that guide people to proactively prevent lung cancer

[0036]At present, Shanghai Zhujian Bioengineering Co., Ltd. has launched this service.

[0037] Step 1: DNA Extraction

[0038] The subjects were consulted before the service, and the subjects signed the informed consent and filled out the questionnaire on living and eating habits. According to the instructions in the sampling box, the oral epithelial cells were sampled with oral swabs, and the DNA of the oral epithelial cells was extracted by silica gel adsorption.

[0039] Step 2: Genotyping Assays

[0040] Using the kit provided by the present invention, the SNP site rs25487 on the XRCC1 gene of the tested person's DNA is detected by fluorescent quantitative PCR, and the genotype of the SNP site is determined.

[0041] Step 3: Instruct people to be proactive in preventing lung cancer

[0042] Through the analysis of the SNP genotype of the tested person, the genotyping test report and the ...

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PUM

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Abstract

The present invention discloses a kit for detecting pulmonary carcinoma susceptibility. Said kit includes specific primer pair and specific fluorescent probe for detecting SNP site of position 156 in ERCC2 gene SEQ ID NO.1 and conventional component for making fluorescent quantitative PCR detection.

Description

technical field [0001] The invention relates to a kit for detecting disease susceptibility, in particular to a kit for detecting susceptibility to lung cancer, by detecting X-ray repair cross--complementing gene 1 (XRCC1) Single nucleotide polymorphism (SNP) loci to predict individual susceptibility to lung cancer. Background technique [0002] Primary bronchial lung cancer, referred to as lung cancer, is one of the most common malignant tumors in the world and a serious threat to human health and life. Tumor cells originate from the bronchial mucosa or glands, and often have regional lymph node and hematogenous metastasis. In the early stage, respiratory symptoms such as irritating cough and blood in the sputum are often present. The speed of disease progression is related to the biological characteristics of the cells. [0003] For half a century, the morbidity and mortality of lung cancer in all countries in the world have a tendency to increase significantly. The World...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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