Reagent kit for detecting susceptibility of cancer of the lungs with SNPs of gene XRCC1 and ERCC2

Abstract

The present invention discloses a kit used for inspection of susceptibility of lung cancer. The kit comprises not only a specific primer couple and a specific fluorescent probe, which are capable of inspecting at the same time the site SNP of No. rs25487 of gene XRCC1, both the site SNP of No. rs13181 and the site SNP of No. rs1799793 of gene ERCC2, but also a standard component used for fluorescent quantitative PCR. The kit provided by the present invention predicts the individual susceptibility to lung cancer through mutual analysis on the gene type on the site of SNPs of both the gene XRCC1 and gene ERCC2.

Description

technical field

[0001] The present invention relates to a kit for detecting disease susceptibility, especially a kit for detecting susceptibility to lung cancer, by simultaneously detecting X-ray repair cross--complementing gene 1 (XRCC1) The single nucleotide polymorphism site (SNP) of the excision repair complex 2 (Excision Repair Cross-complementing Rodent Repair Deficiency, Complementation Group2, ERCC2) two different single nucleotide polymorphism sites (SNPs) to predict individual susceptibility to lung cancer. Background technique

[0002] Primary bronchial lung cancer, referred to as lung cancer, is one of the most common malignant tumors in the world and a serious threat to human health and life. Tumor cells originate from bronchial mucosa or glands, and often have regional lymph node and hematogenous metastasis. In the early stage, respiratory symptoms such as irritating cough and bloody sputum are often present. The speed of disease progression is related to the ...

Examples

Embodiment 1

[0027] Example 1. Use of detection kits

[0028] Step 1: Extraction of DNA template

[0029] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0030] Step 2: Real-time quantitative PCR reaction

[0031] Use a fluorescent quantitative PCR detection kit that can detect susceptibility to lung cancer, which contains the following primer pairs and fluorescent probes:

[0032] Sense primer 1: 5'-TAACTGGCATCTTCACTTCT-3' (SEQ ID NO: 1) with a Tm value of 56°C

[0033] Antisense primer 1: 5'-TCCAGATTCCTGGCATTGC-3' (SEQ ID NO: 2) with a Tm value of 58°C

[0034] Sense primer 2: 5'-ACTCAGGAGTCACCAGGAA-3' (SEQ ID NO: 3) with a Tm value of 58°C

[0035] Antisense primer 2: 5'-TCTGTTCTTCTGCAGGAGGA-3' (SEQ ID NO: 4) Tm value is 58°C

[0036] Sense primer 3: 5'-ATCAAAGAGACAGACGAGCA-3' (SEQ ID NO: 5) with a Tm value of 58°C

[0037] Antisense primer 3: 5'-TCAGGAAGCCCAGGAAAT-3' (SEQ ID NO: 6) with a Tm value of 54°C

[0038] Fluorescent probe 1: 5'-CGGCTGCCCT...

Embodiment 2

[0047] Example 2. Services that guide people to proactively prevent lung cancer

[0048] At present, Shanghai Zhujian Bioengineering Co., Ltd. has launched this service.

[0049] Step 1: DNA Extraction

[0050] The subjects were consulted before the service, and the subjects signed the informed consent and filled out the questionnaire on living and eating habits. According to the instructions in the sampling box, the oral epithelial cells were sampled with oral swabs, and the DNA of the oral epithelial cells was extracted by silica gel adsorption.

[0051] Step 2: Genotyping Assays

[0052] Using the kit provided by the invention, the SNP site No. rs25487 on the XRCC1 gene, the SNP site No. rs13181 on the ERCC2 gene and the SNP site No. rs1799793 on the ERCC2 gene are simultaneously detected by fluorescence quantitative PCR to determine the Genotypes of the three SNPs loci.

[0053] Step 3: Instruct people to be proactive in preventing lung cancer

[0054] Through the ana...

technical field

[0001] The present invention relates to a kit for detecting disease susceptibility, especially a kit for detecting susceptibility to lung cancer, by simultaneously detecting X-ray repair cross--complementing gene 1 (XRCC1) The single nucleotide polymorphism site (SNP) of the excision repair complex 2 (Excision Repair Cross-complementing Rodent Repair Deficiency, Complementation Group2, ERCC2) two different single nucleotide polymorphism sites (SNPs) to predict individual susceptibility to lung cancer. Background technique

[0002] Primary bronchial lung cancer, referred to as lung cancer, is one of the most common malignant tumors in the world and a serious threat to human health and life. Tumor cells originate from bronchial mucosa or glands, and often have regional lymph node and hematogenous metastasis. In the early stage, respiratory symptoms such as irritating cough and bloody sputum are often present. The speed of disease progression is related to the ...

Examples

Embodiment 1

[0027] Example 1. Use of detection kits

[0028] Step 1: Extraction of DNA template

[0029] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0030] Step 2: Real-time quantitative PCR reaction

[0031] Use a fluorescent quantitative PCR detection kit that can detect susceptibility to lung cancer, which contains the following primer pairs and fluorescent probes:

[0032] Sense primer 1: 5'-TAACTGGCATCTTCACTTCT-3' (SEQ ID NO: 1) with a Tm value of 56°C

[0033] Antisense primer 1: 5'-TCCAGATTCCTGGCATTGC-3' (SEQ ID NO: 2) with a Tm value of 58°C

[0034] Sense primer 2: 5'-ACTCAGGAGTCACCAGGAA-3' (SEQ ID NO: 3) with a Tm value of 58°C

[0035] Antisense primer 2: 5'-TCTGTTCTTCTGCAGGAGGA-3' (SEQ ID NO: 4) Tm value is 58°C

[0036] Sense primer 3: 5'-ATCAAAGAGACAGACGAGCA-3' (SEQ ID NO: 5) with a Tm value of 58°C

[0037] Antisense primer 3: 5'-TCAGGAAGCCCAGGAAAT-3' (SEQ ID NO: 6) with a Tm value of 54°C

[0038] Fluorescent probe 1: 5'-CGGCTGCCCT...

Embodiment 2

[0047] Example 2. Services that guide people to proactively prevent lung cancer

[0048] At present, Shanghai Zhujian Bioengineering Co., Ltd. has launched this service.

[0049] Step 1: DNA Extraction

[0050] The subjects were consulted before the service, and the subjects signed the informed consent and filled out the questionnaire on living and eating habits. According to the instructions in the sampling box, the oral epithelial cells were sampled with oral swabs, and the DNA of the oral epithelial cells was extracted by silica gel adsorption.

[0051] Step 2: Genotyping Assays

[0052] Using the kit provided by the invention, the SNP site No. rs25487 on the XRCC1 gene, the SNP site No. rs13181 on the ERCC2 gene and the SNP site No. rs1799793 on the ERCC2 gene are simultaneously detected by fluorescence quantitative PCR to determine the Genotypes of the three SNPs loci.

[0053] Step 3: Instruct people to be proactive in preventing lung cancer

[0054] Through the ana...