Selective culture medium for gardnerella vaginalis and method for producing the same

The technology of Gardnerella vaginalis and culture medium is applied to the selective culture medium of Gardnerella vaginalis and the field of preparation thereof, can solve problems such as unfavorable growth of Gardnerella vaginalis, and achieves convenient preparation, low price and rich nutrition. Effect

Inactive Publication Date: 2008-05-07
绍兴县中心医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is not conduciv

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0034] Medium composition: nutrient broth powder 15g; Columbia agar 39g; Tween 800.3ml; Gram stain 1 solution (crystal violet solution) 0.05ml; distilled water 920ml; , sheep, rabbits can be). Nutrient broth; Columbia agar; Tween 80; Gram stain solution 1 (crystal violet solution); distilled water were added together and stirred evenly, and then autoclaved at 121°C for 20 minutes. Cool to 45-50°C, then add 5-fluorocytosine, human blood, and plasma, shake well, pour the culture medium into a plate, cool and place it upside down in 4-8°C ice for later use; the storage time should not exceed 3 weeks.

[0035] Spread the vaginal secretions containing Gardnerella, Staphylococcus, albicans, Gram-positive cocci, and mold on the above-prepared culture medium, and place in a medium containing 5% to 10% CO 2 Cultured at 35°C-37°C for 24-48 hours, Gardnerella grows well, 0.5-0.8mm in size, lavender, smooth and raised, translucent, producing β-hemolytic colonies. Staphylococcus grows po...

Embodiment 2

[0037] Medium composition: nutrient broth 18g; Columbia agar 44g; Tween 80 0.3ml; Gram stain 1 solution (crystal violet solution) 0.0625ml; distilled water 910ml; 5-fluorocytosine 0.02g; human blood 50ml; plasma 40ml (human , sheep, rabbits can be). Nutrient broth; Columbia agar; Tween 80; Gram stain solution 1 (crystal violet solution); distilled water were added together and stirred evenly, and then autoclaved at 121°C for 20 minutes. Cool to 45-50°C, then add 5-fluorocytosine, human blood, and plasma, shake well, pour the culture medium into a plate, cool and place it upside down in 4-8°C ice for later use; the storage time should not exceed 3 weeks.

[0038] Spread the vaginal secretions containing Gardnerella, Staphylococcus, albicans, Gram-positive cocci, and mold on the above-prepared culture medium, and place in a medium containing 5% to 10% CO 2 Cultured at 35-37°C for 24-48 hours, Gardnerella grows well, 0.5-0.8 mm in size, lavender, smooth and raised, translucent, ...

Embodiment 3

[0040] Medium composition: nutrient broth 18g; Columbia agar 39g; Tween 80 0.3ml Gram stain 1 solution (crystal violet solution) 0.05ml; distilled water 900ml; 5-fluorocytosine 0.03g; human blood 50ml; plasma 50ml (human, Sheep and rabbits are acceptable). Nutrient broth; Columbia agar; Tween 80; Gram stain solution 1 (crystal violet solution); distilled water were added together and stirred evenly, and then autoclaved at 121°C for 20 minutes. Cool to 45-50°C, then add 5-fluorocytosine, human blood, and plasma, shake well, pour the culture medium into a plate, cool and place it upside down in 4-8°C ice for later use; the storage time should not exceed 3 weeks.

[0041] Spread the vaginal secretions containing Gardnerella, Staphylococcus, albicans, Gram-positive cocci, and mold on the above-prepared culture medium, and place in a place containing 5% to 10% CO 2 Cultured at 35-37°C for 24-48 hours, Gardnerella grows well, 0.5-0.8 mm in size, lavender, smooth and raised, translu...

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Abstract

The invention discloses a selective culture medium of Gardnerella vaginalis. Preparation method for 1000ml culture medium: 15 to 20g nutrient broth, 35 to 44g Columbia agar bess, 0.2 to 0.4 Tween 80, 0.05-0.075ml Gram's stain I solution (crystal violet), 0.02-0.03g 5-Fluorocytosine, 50-80ml human blood, 30-50ml plasma; distilled water is added to 1000ml. The invention has the advantages of ability to effectively separate the Gardnerella vaginalis from Gram-positive staphylococcus, streptococcus, Gram positive bacilli and various mould, quickened detection of Gardnerella vaginalis, rich nutrient, low cost and convenient preparation.

Description

technical field [0001] The invention relates to a selective culture medium for Gardnerella vaginalis and a preparation method thereof. Background technique [0002] Gardnerella vaginalis (Gardnerella vaginalis, GV) is the most common pathogen of female bacterial vaginosis (Bacterial Vaginosis, BV), one of the pathogens of sexually transmitted diseases, since 1955 by Gardner and Dukes from patients with non-specific vaginitis Since the strain was isolated from the specimen, there have been reports of genitourinary system infection caused by this bacteria at home and abroad. Currently, due to the high nutritional requirements of Gardnerella vaginalis, the first isolation requires the provision of CO 2 Or an anaerobic environment, which brings difficulties to the routine separation of laboratories. For this reason, there are not many hospitals in China that carry out the cultivation of Gardnerella vaginalis, which is not conducive to the treatment of vaginosis. [0003] Cultu...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 吕旭军陈建江夏景洋茅利明吴先华陈静朱遐
Owner 绍兴县中心医院
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