Core-peptide composition with physiological activity and its preparation method and application

A peptide complex, physiologically active technology, applied in the field of biomedicine, to achieve the effect of remarkable purification effect, easy operation and improving the level of free testosterone

Active Publication Date: 2008-08-13
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] Clearly, androgen replacement therapy is

Method used

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  • Core-peptide composition with physiological activity and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Take 100g of red snail tissue (trunk, liver, reproductive organs) and mash it in a frozen state, add 10 times the amount of 95% ethanol to extract for 1 hour, filter, then stir and extract with 50% acidic ethanol for 3 hours, filter, combine the two extracts, Let cool, precipitate out, pour out the supernatant, concentrate, treat with 3 times the amount of hexane, discard the hexane layer, purify the lower lipoprotein layer with 2% activated carbon, ultrafilter, concentrate, and keep the operating temperature below 40 ℃, add vitamin E to make the final concentration to 0.03%, concentrate under reduced pressure to remove the solvent, obtain the crude product, and then use sephadex LH20 column chromatography to separate, the volume ratio is 5: 3 methanol-water composite solvent (use acetic acid to adjust the eluent to be acidic) ) to elute the nucleopeptide complex.

[0049] After chemical analysis and detection, the obtained nucleopeptide complex components include per m...

Embodiment 2

[0051] Take 100g of the whole tissue of the red snail and mash it in the frozen state, add 10 times the amount of 50% ethanol, adjust the pH value to not less than 4.5 with acetic acid, extract it in a container with mixing and stirring function for 2 hours, filter, concentrate, and let it cool The precipitate was precipitated, the supernatant was poured out, treated with 3 times the amount of hexane, the hexane layer was discarded, the lower lipoprotein layer was purified with 2% activated carbon, ultrafiltered, concentrated, the operating temperature was always kept below 40°C, and 0.02% Vitamin E was concentrated under reduced pressure to remove the solvent to obtain a crude product, which was then separated by sephadex LH20 column chromatography and eluted with a methanol-water composite solvent with a volume ratio of 5:2 to obtain a nucleopeptide complex.

[0052] After chemical analysis and detection, the obtained nucleopeptide complex components include per milliliter: 8...

Embodiment 3

[0054] Take 100g of snail tissues (trunk, liver, reproductive organs) and crush them in a frozen state, add 8 times the amount of 80% ethanol, adjust the pH value to not less than 4.5 with acetic acid, stir and extract for 3 hours, filter, and concentrate to 1 / 2 of the original volume 10. Let the precipitate cool down, pour out the supernatant, treat with 3 times the amount of hexane, discard the hexane layer, purify the lower lipoprotein layer with 2% activated carbon, ultrafilter, concentrate, and keep the operating temperature below 40°C. , add 0.03% vitamin E, concentrate under reduced pressure to remove the solvent to obtain the crude product, then use sephadex LH20 column chromatography to separate, the volume ratio is 5:4 ethanol-water composite solvent (use acetic acid to adjust the eluent to be acidic) and elute to obtain the nucleus peptide complex.

[0055] After chemical analysis and detection, the obtained nucleopeptide complex components include per milliliter: 8...

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Abstract

The invention relates to a natural composite with biological activity extracted from conch, and application in the pharmaceutical field of the same, pertains to biological and pharmaceutical technical fields. The invention protects a natural composite with biological activity, preparation and application thereof. The invention is extracted from marine organism of conch, has a unique biological activity, can notably content of primotest in PADAM patient's blood.

Description

technical field [0001] The invention relates to a natural physiologically active nucleopeptide complex extracted from marine snails, a preparation method of the complex and an application in medicine, belonging to the technical field of biomedicine. Background technique [0002] Men start from the age of 50, and with age, testicular metabolic physiological decline, androgen synthesis function decline, severe cases cause partial androgen deficiency syndrome (Partial Androgen Deficiency in Aging Male, PADAM) in middle-aged and elderly men, the syndrome is characterized by Changes in the physical and mental domains that are related to and corrected by modulating the androgenic environment. [0003] In 2002, the International Society for the Study of Aging Men (ISSAM) renamed this syndrome as late onset hypogonadism in males (LOH), and defined this disease as a disease that occurs in middle-aged and elderly men with age. associated hypotesticular function and has been endorsed ...

Claims

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Application Information

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IPC IPC(8): C07K2/00C07K1/14C07K1/16A61K38/02A61P5/26
Inventor 刘可春达特森科·卓娅考米沙林科·谢尔盖契克曼·伊万康尼维特·娜塔丽娅莫伊谢耶娃·柳米拉韩利文柳高夫斯卡依士丘克亚斯尼
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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