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Fortifier, special bait, injection for bleeding disease immunity of Chinese idle and novel immunity method

A grass carp haemorrhagic disease and enhancer technology, applied in antiviral agents, applications, animal feed, etc., can solve the problems of vaccine antigenicity easily damaged by digestive juice, unsatisfactory effect, time-consuming and labor-intensive, etc., to achieve enhanced immune prevention effect, The effect of reducing the degree of structural damage and strengthening the immune effect

Inactive Publication Date: 2008-09-24
FISHERIES RES INST ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(1) Injection is time-consuming and labor-intensive and difficult to operate because it is easy to cause fish body injury, and it is more difficult to operate in the fry stage, which is greatly restricted; (2) Bathing is also a commonly used way of administration at present, but there are technical problems. Still need difficult problems such as pre-treatment and poor immune effect, economically also be subject to be widely used in production because of the restriction of high cost; Size constraints can also reduce labor intensity, but usually the vaccine is easily lost when mixed with general bait, and the antigenicity of the vaccine is easily damaged by digestive juice after entering the fish body. The large amount of vaccine used and poor immune effect have limited its application.
For aquatic organisms growing in water bodies, the traditional vaccine prevention method is inconvenient in vaccination operation, and the effect is not ideal
Based on the above, these three methods have different limitations. Therefore, it is an urgent research topic to seek a new method that is more convenient and effective for grass carp immunization.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Get the raw material of following weight portion:

[0056] 90 grams of chitosan (deacetylated product), 8 grams of beta-glucan, 1 gram of vitamin C and 1 gram of vitamin E are fully mixed to obtain the enhancer for grass carp hemorrhagic disease immunity.

[0057] Get the raw material of following weight portion:

[0058] The above-mentioned 2 parts of grass carp haemorrhagic disease immunization enhancer, 60 parts of rotifers, 30 parts of egg yolk and 8 parts of starch are fully mixed to obtain the preparatory bait; the preparatory bait and grass carp hemorrhagic disease vaccine are fully mixed and adsorbed for more than 15 minutes to obtain specific Sexual bait, used as opening bait for grass carp fry;

[0059] Grass carp hemorrhagic disease vaccine is attenuated grass carp freeze-dried cell vaccine produced by Pearl River Fisheries Research Institute, and the dosage is according to the manufacturer's requirements. Grass carp haemorrhagic disease immunization enhanc...

Embodiment 2

[0061] Get the raw material of following weight portion:

[0062] 95 grams of chitosan (deacetylated product), 9 grams of beta-glucan, 2 grams of vitamin C and 2 grams of vitamin E, are fully mixed to obtain the enhancer for grass carp hemorrhagic disease immunity.

[0063] Get the raw material of following weight portion:

[0064] The above-mentioned 2.2 parts of enhancer for grass carp hemorrhagic disease immunity, 55 parts of rotifers, 28 parts of egg yolk and 9 parts of starch are fully mixed to obtain the preparatory bait; the preparatory bait and the grass carp hemorrhagic disease vaccine are fully mixed and adsorbed for more than 15 minutes to obtain specific Sexual bait, used as opening bait for grass carp fry;

[0065] Others are with embodiment 1.

Embodiment 3

[0067] Get the raw material of following weight portion:

[0068] 100 grams of chitosan (deacetylated product), 10 grams of β-glucan, 3 grams of vitamin C and 3 grams of vitamin E, are thoroughly mixed to obtain the enhancer for grass carp hemorrhagic disease immunity.

[0069] Get the raw material of following weight portion:

[0070] The above-mentioned 2.5 parts of enhancer for grass carp hemorrhagic disease immunization, 65 parts of rotifers, 35 parts of egg yolk and 10 parts of starch are fully mixed to obtain the preparatory bait; the preparatory bait and the grass carp hemorrhagic disease vaccine are fully mixed and adsorbed for more than 15 minutes to obtain specific Sexual bait, used as opening bait for grass carp fry immunity;

[0071] Others are with embodiment 1.

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PUM

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Abstract

The invention relates to grass carp hemorrhage immunizing intensifier, specific bait, injection, and new immunity method. The invention aims at overcoming the defects existing in immunity and inoculability against the main diseases in the young fry stage. The immunizing intensifier is prepared by chitosan, beta-glucan, vitamin C and vitamin E. The weaning specific bait for the grass carp fry is prepared by using the above immunizing intensifier, rotifer, vitelline, and fecula to make preparative bait, mixing the preparative bait and relevant multi-vaccine fully to make the weaning bait for grass carp fry. Adding the immunizing intensifier of the invention in the hemorrhage bacterin can promote the immunity effect; by injecting the grass carp hemorrhage bacterin and the immunizing intensifier in the abdominal cavity of the female ripe fish 30-45 days before the grass carp parents propagating for primary pre-immunity, with the specific bait which is made from fry weaning bait using the rotifer and the like to adsorb the bacterin, the immunizing and preventing effect is strengthened, and the bacterin dosage is reduced. The invention solves the problems that the operation of immunity inoculability for the grass carp at the young fry stage is difficult and the effect is poor.

Description

technical field [0001] The invention relates to improving the immune effect of grass carp hemorrhagic disease, in particular to the method of applying immune enhancer, specific bait and injecting hemorrhagic disease vaccine in grass carp broodstock during the immunization process of grass carp hemorrhagic disease. Background technique [0002] Grass carp aquaculture has developed rapidly with the promotion of intensive farming models. However, due to problems such as high-density breeding, increased feeding frequency, and water pollution, the probability of being infected with various diseases has greatly increased, which has caused huge losses to the breeding industry. Especially the outbreak of epidemic hemorrhagic disease often brings a devastating blow to the breeding industry in some areas. Therefore, it has become one of the key technologies for the healthy development of the current grass carp aquaculture industry in my country to strengthen the research on the preve...

Claims

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Application Information

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IPC IPC(8): A61K39/39A23K1/18A61P31/12A23K20/163A23K20/174A23K50/80
Inventor 王永杰陈宇李艳和潘庭双卢文轩佘磊
Owner FISHERIES RES INST ANHUI ACAD OF AGRI SCI
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