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Tissue culture method for rapid propagation of Dendrobium candidum

A technology of Dendrobium officinale and a culture medium, applied in the biological field, can solve the problems of low natural reproduction ability, high production cost, single provenance of Dendrobium officinale, etc., and achieves the development and robustness of the root system of the cultured plant, the more root system of the cultured plant, the higher the The effect of transplanting survival rate

Inactive Publication Date: 2008-10-01
南京养颐堂生物科技实业有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] But Dendrobium candidum is extremely low in natural reproductive capacity, and the amount of collection is far greater than its growth. At present, natural resources are becoming increasingly exhausted; therefore, it is of special significance and Application prospects
[0004] In recent years, some scientific research institutions and enterprises in my country have continuously explored and tested the tissue culture and artificial cultivation techniques of Dendrobium officinale, and have achieved certain results. But its natural reproduction rate is low and limited by seasons
The main problems such as slow propagation speed and high production cost have affected the industrial production of Dendrobium officinale

Method used

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  • Tissue culture method for rapid propagation of Dendrobium candidum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: as figure 1 As shown, first, wild Dendrobium candidum or field-cultivated Dendrobium candidum were selected to collect stem buds, and after routine disinfection procedures, they were put into N6 basic medium + indole butyric acid 1.5mg / L+6-benzyl adenine 0.5mg / L+ Carrageenan 7g / L + soft white sugar 40g / L + 50g / L potato juice induction medium, placed in an environment with a temperature of 22-25°C, a light of 2000-3000lx, and a daily light of 10-12h for protocorm After induction culture, the formation of protocorms can be observed after 2 months.

[0026] Put the formed protocorm into the differentiation medium prepared by N6 basic medium + 6-benzyl adenine 2.0mg / L + carrageenan 7g / L + soft white sugar 20g / L + 50g / L potato juice, place The temperature is 22-25°C, the light is 2000-3000lx, and the daily light is 10-12h for differentiation culture. After 2 months of culture, it can be observed that the protocorms in the bottle have undergone redifferentiatio...

Embodiment 2

[0028] Embodiment 2: as figure 1 As shown, first select the artificially cultivated Dendrobium candidum to collect stem buds, and after routine disinfection procedures, put them into N6 basic medium + indole butyric acid 1.5mg / L + 6-benzyl adenine 0.5mg / L + carrageenan 8g / L+soft white sugar 30g / L+100g / L potato juice preparation medium, placed in the temperature of 22-25 ℃, light 2000-3000lx, daily light 10-12h environment for protocorm induction culture. Protocorm formation was observed after 2 months.

[0029] Put the formed protocorm into the differentiation medium prepared by N6 basic medium + 6-benzyl adenine 2.0mg / L + carrageenan 8g / L + soft white sugar 30g / L + 100g / L potato juice, and place it at temperature The temperature is 22-25°C, the light is 2000-3000lx, and the daily light is 10-12h for differentiation culture. After 2 months of cultivation, it can be observed that the protocorms in the bottle have undergone redifferentiation to form seedlings, and the height o...

Embodiment 3

[0031] Embodiment 3: as figure 1 As shown, first, wild Dendrobium candidum or field-cultivated Dendrobium candidum were selected to collect stem buds, and after routine disinfection procedures, they were put into N6 basic medium + indole butyric acid 1.5mg / L+6-benzyl adenine 0.5mg / L+ Carrageenan 8.5g / L + soft white sugar 30g / L + 150g / L potato juice prepared induction medium, placed in the environment of temperature 22-25 ℃, light 2000-3000lx, daily light 10-12h After corm induction culture, the formation of protocorms can be observed after 2 months.

[0032] Put the formed protocorm into the differentiation medium configured by N6 basic medium+6-benzyl adenine 2.0mg / L+carrageenan 8.5g / L+soft white sugar 40g / L+150g / L potato juice, place Carry out differentiation culture at a temperature of 22-25°C, light of 2000-3000 lx, and light of 10-12 hours per day. After 2 months of cultivation, it can be observed that the protocorms in the bottle have undergone re-differentiation to for...

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Abstract

The invention discloses a tissue culture method of dendrobium candidum rapid propagation which relates to the field of biological technique. The stemeye of dendrobiun candidum is first selected for induction culture in a protocorm induction culture medium, after two months, the protocorm can be formed and observed; then the stemeye is put in a differential medium for differentiation culture, after two months, the protocorm in a bottle can be observed to form plantlets with the height of about 1cm by redifferentiation; the differentiated plantlets are connected and put in a mature shoot culture medium for mature shoot culture, after about three months, that the uniformity of the mature seedlings of the dendrobiun candidum is in line, each seedling has at least 2-3 developed and lusty root systems, the height of plants is 3 to 6cm and the vane is relatively green, which can be observed in the bottle. The tissue culture method of the invention can shorten the culture time of dendrobiun candidum, not only effectively solves the source problem of seed source of the dendrobiun candidum, is also not limited by time and places; compared with the traditional germchit enlarging propagation, the speed is improved and the time is shortened to be 7 months and carrageenan is used for substituting for gelose, thus saving cost.

Description

1. Technical field: [0001] The invention relates to a biological propagation method, which belongs to the field of biotechnology, in particular to a tissue culture method for rapid propagation of Dendrobium officinale. 2. Background technology: [0002] Dendrobium candidum is the species with the highest economic value in the Dendrobium genus at the present stage. Its use is mainly for medicinal and health products. Tail-shaped "Tiepi Fengdou), Tiepi Fengdou Crystal (health drink), Tiepi Fengdou capsules, Yin-Yin Yiqi capsules. Clinical studies of traditional Chinese medicine and modern pharmacological studies have proved that Dendrobium officinale has the effects of promoting body fluids, quenching thirst, analgesia, and eliminating edema in medicine, and can improve immune function, anti-aging, prevent and treat tumors and cardiovascular diseases, improve sleep, and prevent and treat cataracts. and other disease functions. [0003] But Dendrobium candidum is extremely lo...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 王小霞钟玉梅胡春宏
Owner 南京养颐堂生物科技实业有限公司
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