Immune colloidal gold reagent for detecting orchid virus series and its preparation

A colloidal gold test paper and colloidal gold technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of high testing costs, long testing time, and inability to detect on-site, and achieve fast detection speed, easy operation, and production low cost effect

Inactive Publication Date: 2008-10-29
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
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Problems solved by technology

[0007] The task of the present invention is to solve the above-mentioned shortcomings such as long detection time, inability to detect on-site, and high detection costs, and provide a specificity, high sensitivity, si

Method used

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Experimental program
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Embodiment

[0045] Embodiment: the preparation method of the immune colloidal gold test strip that detects TMV virus may further comprise the steps:

[0046] 1) Polyclonal antibody: inject the purified tobacco mosaic virus into rabbits, collect blood and serum, and purify the polyclonal antibody by affinity chromatography;

[0047] 2) Preparation of colloidal gold: 100ml of 0.01% gold chloride is reduced to particles of 15nm-40nm with 2-3ml of 1% trisodium citrate;

[0048] 3) Polyclonal antibody colloidal gold marker: use 0.1mol / L K 2 CO 3 Adjust the pH value of the colloidal gold solution to about 7.8, and mix the colloidal gold solution and the polyclonal antibody at a ratio of 0.5mg-2mg polyclonal antibody to 100ml of the colloidal gold solution, so that the colloidal gold and the antibody form a stable colloidal gold complex After centrifuging, discarding the supernatant, washing, and re-concentrating, colloidal gold markers are formed, and they are refrigerated for later use;

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PUM

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Abstract

The invention discloses an immune colloidal gold reagent used for detecting tobacco virus series and preparing method thereof, comprising an immune colloidal gold test strip used for detecting TMV virus and preparing method thereof, an immune colloidal gold test strip used for detecting CMV virus and preparing method thereof and an immune colloidal gold test strip used for detecting PVY virus and preparing method thereof; the preparing method has simple production process and low cost; the products have high sensitivity and good repetitiveness; the cost used for detection is only 1/5 to 1/10 of that of the RT-PCR and is much cheaper than an enzyme-labeled reagent kit; the detection has fast speed with the whole process being only 30 minutes, can be carried out on spot, and has the advantages of accurate result, fast speed, simple operation and easy promotion and use.

Description

technical field [0001] The invention relates to a reagent for detecting tobacco mosaic virus (TMV), cucumber mosaic virus (CMV) and potato virus Y (PVY) by immunocolloidal gold method and a preparation method thereof. Background technique [0002] Tobacco mosaic virus (Tobacco mosaic virus, TMV) is an important plant virus that harms tobacco, and its host range is very wide. It can infect at least 199 plants of 30 species. The potential plant species that can be infected are more wide. It occurs in every smoking area, not only causing the loss of tobacco production, but also seriously degrading the quality of tobacco, especially the proportion and internal quality of middle and high-grade tobacco, and causing serious economic losses. But now there is no cure drug available, so establishing a rapid virus detection program to find out the diseased strains and carry out artificial destruction or isolation has become one of the effective measures to prevent the further spread a...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/544G01N33/532G01N33/52
Inventor 吴祖建林志铿谢联辉谢荔岩
Owner FUJIAN AGRI & FORESTRY UNIV
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