Dendritic cell vaccine for promoting spinal cord injury function recovery

A technology for dendritic cells and spinal cord injury, which is applied to vertebrate cells, animal cells, artificial cell constructs, etc., and can solve the problems such as the need for special equipment for trauma models, unfavorable for clinicians to master and study, and complex interaction relationships.

Inactive Publication Date: 2008-12-31
THE THIRD AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIV OF PLA
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Problems solved by technology

[0005] The role of DCs in SCI has been affirmed, but there are still some problems in the current research: First, the animal models used in the research are mostly spinal cord cut injury models, which are mostly caused by compression of fracture fragments, ligaments, intervertebral discs or hematoma in clinical patients. The pathological type of contusion injury does not match, which affects the clinical application of research results; the trauma model requires special equipment, human error is lar...

Method used

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  • Dendritic cell vaccine for promoting spinal cord injury function recovery
  • Dendritic cell vaccine for promoting spinal cord injury function recovery
  • Dendritic cell vaccine for promoting spinal cord injury function recovery

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Embodiment 1

[0053] Embodiment 1, the operating steps of the dendritic cell vaccine preparation method

[0054] 1. Acquisition of bone marrow cells: Two BALB / C mice were taken, killed by cervical dislocation, soaked in 75% ethanol for 3 minutes, fixed the mice on an ultra-clean table, sterilized, peeled off the skin, removed the muscles, and took out the femur and The tibia was washed with PBS, fixed in 75% ethanol for 3 minutes, washed with PBS, and placed in RPMI 1640 culture medium for later use. Fix the backbone with tweezers, cut off both ends of the bone with scissors, and then use a sterile empty needle to extract RPMI 1640 culture solution to wash the bone marrow cavity, repeat 2-3 times, wash the bone marrow cells into the culture dish, and finally put the bone marrow cells in the culture dish The RPMI 1640 culture solution was filtered through sterile cotton balls to remove its tissue components. Collect the filtered RPMI 1640 culture medium containing bone marrow cells, centrif...

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Abstract

The invention relates to a dendritic cell bacterin, in particular to a dendritic cell bacterin which can effectively promote the functional recovery of spinal cord injury and a manufacturing method thereof.

Description

technical field [0001] The invention relates to a dendritic cell vaccine, in particular to a dendritic cell vaccine capable of effectively promoting recovery of spinal cord injury function and a preparation method thereof. Background technique [0002] The total incidence of spinal cord injury (spinal cord injury, SCI) in the world is 20-40 people / million people / year. The incidence rate in the United States is 28-55 people / million people / year, with about 10,000 new cases every year, the average age at the time of injury is 31.7 years, and there are about 230,000 SCI patients. The lifetime cost of individual patients is about 500,000-2 million US dollars, and the total annual care and treatment costs exceed 7 billion US dollars. There is no comprehensive statistics in our country, but it is estimated that it will not be lower than this level. [0003] The mechanical violence of acute spinal cord injury includes compressive force and tractive force. Direct compressive viole...

Claims

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Application Information

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IPC IPC(8): A61K39/00C12N5/06C12N5/08A61P19/00C12N5/071C12N5/0784
Inventor 梁华平刘明永赵建华
Owner THE THIRD AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIV OF PLA
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