Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Dendritic cell vaccine for promoting spinal cord injury function recovery

A technology for dendritic cells and spinal cord injury, which is applied to vertebrate cells, animal cells, artificial cell constructs, etc., and can solve the problems such as the need for special equipment for trauma models, unfavorable for clinicians to master and study, and complex interaction relationships.

Inactive Publication Date: 2008-12-31
THE THIRD AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIV OF PLA
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The role of DCs in SCI has been affirmed, but there are still some problems in the current research: First, the animal models used in the research are mostly spinal cord cut injury models, which are mostly caused by compression of fracture fragments, ligaments, intervertebral discs or hematoma in clinical patients. The pathological type of contusion injury does not match, which affects the clinical application of research results; the trauma model requires special equipment, human error is large, and it is difficult to standardize; second, the preparation of MBP is complicated, which is not conducive to clinicians' mastering and research; third, using The self-antigens are more and more specific, most of which are a single peptide segment, but in SCI, multiple tissue antigen components coexist in the body, and the interaction relationship is complex, and there may be multiple additive or subtractive effects, and a single antigen component cannot reflect the real situation in the body. Happening

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Dendritic cell vaccine for promoting spinal cord injury function recovery
  • Dendritic cell vaccine for promoting spinal cord injury function recovery
  • Dendritic cell vaccine for promoting spinal cord injury function recovery

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1, the operating steps of the dendritic cell vaccine preparation method

[0054] 1. Acquisition of bone marrow cells: Two BALB / C mice were taken, killed by cervical dislocation, soaked in 75% ethanol for 3 minutes, fixed the mice on an ultra-clean table, sterilized, peeled off the skin, removed the muscles, and took out the femur and The tibia was washed with PBS, fixed in 75% ethanol for 3 minutes, washed with PBS, and placed in RPMI 1640 culture medium for later use. Fix the backbone with tweezers, cut off both ends of the bone with scissors, and then use a sterile empty needle to extract RPMI 1640 culture solution to wash the bone marrow cavity, repeat 2-3 times, wash the bone marrow cells into the culture dish, and finally put the bone marrow cells in the culture dish The RPMI 1640 culture solution was filtered through sterile cotton balls to remove its tissue components. Collect the filtered RPMI 1640 culture medium containing bone marrow cells, centrif...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a dendritic cell bacterin, in particular to a dendritic cell bacterin which can effectively promote the functional recovery of spinal cord injury and a manufacturing method thereof.

Description

technical field [0001] The invention relates to a dendritic cell vaccine, in particular to a dendritic cell vaccine capable of effectively promoting recovery of spinal cord injury function and a preparation method thereof. Background technique [0002] The total incidence of spinal cord injury (spinal cord injury, SCI) in the world is 20-40 people / million people / year. The incidence rate in the United States is 28-55 people / million people / year, with about 10,000 new cases every year, the average age at the time of injury is 31.7 years, and there are about 230,000 SCI patients. The lifetime cost of individual patients is about 500,000-2 million US dollars, and the total annual care and treatment costs exceed 7 billion US dollars. There is no comprehensive statistics in our country, but it is estimated that it will not be lower than this level. [0003] The mechanical violence of acute spinal cord injury includes compressive force and tractive force. Direct compressive viole...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K39/00C12N5/06C12N5/08A61P19/00C12N5/071C12N5/0784
Inventor 梁华平刘明永赵建华
Owner THE THIRD AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIV OF PLA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com