Kit for detecting 5 fluorouracil medicine insensitive gene chip

Abstract

The invention provides a 5-fluorouracil drug sensitivity gene chip detection kit, which can be used to parallelly and economically detect genes related to 5-fluorouracil drug sensitivity. The kit comprises an extraction solution, a hybridization buffer solution, a washing liquor, an amplifying solution, Taq enzyme and a gene chip, the amplifying solution contains a primer for amplifying four genes containing mutant sites, and the four genes include a DPD gene containing IVS14 A / G mutant sites, a GSTPi gene containing Ile105Val mutant sites, an MTHFR gene containing C677T mutant sites, and an XRCC1 gene containing Arg399Glu mutant sites. The 5 fluorouracil is a first-line anti-tumor chemotherapeutic drug, and the detection kit can be used for detecting the four gene mutation conditions sensitive to the 5 fluorouracil drug.

Description

technical field [0001] The invention belongs to the technical field of gene chips for clinical detection, and in particular relates to a detection kit for a 5-fluorouracil drug-sensitive gene chip. Background technique [0002] 5-fluorouracil is a first-line anticancer chemotherapy drug. The drug-sensitive genes involved in 5-fluorouracil include DPD (dihydrouracil dehydrogenase gene), GSTPi (glutathione transferase i), MTHFR (5,10 methylenetetrahydrofolate reductase) and XRCC1 (X Line repair cross-complementing protein 1) four genes. [0003] Dihydropyrimidine dehydrogenase is an enzyme mainly involved in the catabolism of pyrimidine and is the rate-limiting enzyme in the metabolism of 5-fluorouracil. The change of the first base of the 14th intron of the gene from G to A will cause mRNA splicing changes, resulting in the deletion of 165 bases in the 14th exon, thereby inactivating the enzyme and affecting 5-fluorouracil The catabolism of this drug causes the accumulatio...

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Problems solved by technology

The equipment and instruments required by these methods are expensive and technically complex, and are not suitable for promotion as conventional methods from economic considerations.

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