Kit for detecting 5 fluorouracil medicine insensitive gene chip

A drug-sensitive and genetic detection technology, applied in the direction of determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of complex technology, economic discomfort promotion, expensive equipment and instruments, etc.

Inactive Publication Date: 2008-12-31
上海裕隆生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The equipment and instruments required by these methods are expensive and technically complex, and are not suitable for promotion as conventional methods from economic considerations.

Method used

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  • Kit for detecting 5 fluorouracil medicine insensitive gene chip
  • Kit for detecting 5 fluorouracil medicine insensitive gene chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0049] The implementation of the object of the present invention will be described below in conjunction with the accompanying drawings and examples.

[0050] Implementation example:

[0051] 1. Sample Processing

[0052] Oral mucosal exfoliated cells were used as a sample source for genomic DNA extraction. For extraction, Biotech's Cell Genome Extraction Kit was used.

[0053] 2. Chip preparation, spotting the labeled positioning probes in the literature on ultra-flat glass slides, the probe sequences are as follows:

[0054] pos1: TGATTGTAGTATGTATTGATAAAG

[0055] pos2: GATTTGAAGATTATTGGTAATGTA

[0056] pos3: GATTTGATTGTAAAAAGATTGTTGA

[0057] pos4: GTAAGTAATGAATGTAAAAGGATT

[0058] pos5: TGATAAAGTGATAAAGGATTAAAG

[0059] pos6: GTATTTGAGTAAGTAATTGATTGA

[0060] pos7: TGAAAAAGTGTAGATTTTGAGTAA

[0061] pos8: TTGATAATGTTTGTTTGTTTGTAG

[0062] 3. Primer design:

[0063] (1) Design the following primer sequence group A to amplify the gene fragments related to the drug sen...

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Abstract

The invention provides a 5-fluorouracil drug sensitivity gene chip detection kit, which can be used to parallelly and economically detect genes related to 5-fluorouracil drug sensitivity. The kit comprises an extraction solution, a hybridization buffer solution, a washing liquor, an amplifying solution, Taq enzyme and a gene chip, the amplifying solution contains a primer for amplifying four genes containing mutant sites, and the four genes include a DPD gene containing IVS14 A / G mutant sites, a GSTPi gene containing Ile105Val mutant sites, an MTHFR gene containing C677T mutant sites, and an XRCC1 gene containing Arg399Glu mutant sites. The 5 fluorouracil is a first-line anti-tumor chemotherapeutic drug, and the detection kit can be used for detecting the four gene mutation conditions sensitive to the 5 fluorouracil drug.

Description

technical field [0001] The invention belongs to the technical field of gene chips for clinical detection, and in particular relates to a detection kit for a 5-fluorouracil drug-sensitive gene chip. Background technique [0002] 5-fluorouracil is a first-line anticancer chemotherapy drug. The drug-sensitive genes involved in 5-fluorouracil include DPD (dihydrouracil dehydrogenase gene), GSTPi (glutathione transferase i), MTHFR (5,10 methylenetetrahydrofolate reductase) and XRCC1 (X Line repair cross-complementing protein 1) four genes. [0003] Dihydropyrimidine dehydrogenase is an enzyme mainly involved in the catabolism of pyrimidine and is the rate-limiting enzyme in the metabolism of 5-fluorouracil. The change of the first base of the 14th intron of the gene from G to A will cause mRNA splicing changes, resulting in the deletion of 165 bases in the 14th exon, thereby inactivating the enzyme and affecting 5-fluorouracil The catabolism of this drug causes the accumulatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 穆海东汪宁梅杜喜玲
Owner 上海裕隆生物科技有限公司
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