Adjustable pouring type vascular tissue engineering reactor having cultivation cavity rotation and vas stretch functions

Abstract

The invention relates to a vascular tissue engineering reactor which comprises intravascular perfusion circuits (101, 102, 105, 110), a vascular tissue culture cavity (107), a culture cavity rotation driving motor (113) with the rotation driving force which is transferred to the culture cavity (107) to cause the culture cavity to rotate, a vascular reciprocating tension driving device (114) with the reciprocating driving force which acts on a vascular tissue (108) arranged between an outlet pipeline (110) and an inlet pipeline (105) of the culture cavity of the intravascular perfusion circuit to cause the vascular tissue (108) to receive the reciprocating tension along the axial direction. The vascular tissue engineering reactor can simultaneously realize intravascular perfusion of the culture solution, extravascular perfusion of the culture solution the rotation of the culture cavity, the reciprocating tension of the blood vessels in culture along the axial direction, the pulsation of nutrient solution in the vascular tissue in culture and any combination thereof.

Description

technical field [0001] The invention relates to the fields of cell culture and tissue engineering, and more specifically relates to a vascular tissue engineering reactor with the functions of culturing chamber rotation and vascular tissue stretching. Background technique [0002] So far, autografts, allogeneic blood vessels, xenograft blood vessels, and artificially synthesized blood vessels have not been able to become ideal arterial blood vessel substitutes (especially blood vessels with a caliber of less than 6mm), but in recent years, arterial blood vessel tissue engineering bioreactors have been used to construct And research into tissue-engineered arterial vessels brings hope to the field. At present, the research content of bioreactors for arterial tissue engineering mainly includes: [0003] 1. Realize and control the uniform and high-density seeding of cells in the scaffold for vascular tissue: recent studies have found that direct perfusion bioreactors are superio...

AI Technical Summary

Problems solved by technology

[0012] In summary, there are relatively large defects in the existing vascular tissue engineering reactors: the first is that factors such as flow impedance, compliance (that is, the elasticity and buffering capacity of the vessel wall), resistance, flow inertia, etc. The simple simulation of the flow dynamics environment cannot provide a near-physiological flow environment similar to the pulsating flow state of blood in the artery; the second is that it cannot provide the vascular tissue with mechanical mechanisms such as periodic axial stretching, radial stretching, and twisting that approximate the physiological state at the same time. The third is that most of them use the method of no wall rotation, which needs to be improved in terms of uniform cell, high-density seeding and mass transfer.

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