Adjustable pouring type vascular tissue engineering reactor having rotating cultivation cavity

Abstract

The invention provides a vascular tissue engineering reactor which has the functions of culture cavity rotation, dual perfusion inside and outside vascular tissues and intravascular nutrient solution pulsation. The reactor can simultaneously realize the intravascular perfusion of the culture solution, the extravascular perfusion of the culture solution, the rotation of a culture cavity, the reciprocating tension of blood vessels in culture along the axial direction and any combination thereof. In addition, the reactor can realize multi-blood vessels culture at the same time and handling, recording and/or displaying of the pressure data.

Description

technical field [0001] The present invention relates to the fields of cell culture and tissue engineering, and more specifically to a vascular tissue engineering reactor with a culture chamber rotation function. Background technique [0002] So far, autografts, allograft blood vessels, xenogeneic blood vessels, and synthetic blood vessels have not been able to become ideal arterial blood vessel substitutes (especially blood vessels with a diameter of less than 6 mm). However, in recent years, arterial blood vessels have been constructed using tissue engineering bioreactors. and research into tissue-engineered arterial vessels bring hope to this field. At present, the research content of bioreactors for arterial vascular tissue engineering mainly includes: [0003] 1. Achieve and control the uniform and high-density seeding of cells in the scaffold for vascular tissue: recent studies have found that direct perfusion bioreactors are superior to indirect perfusion bioreactors ...

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Problems solved by technology

[0012] In summary, there are relatively large defects in the existing vascular tissue engineering reactors: the first is that factors such as flow impedance, compliance (that is, the elasticity and buffering capacity of the vessel wall), resistance, flow inertia, etc. The simple simulation of the flow dynamics environment cannot provide a near-physiological flow environment similar to the pulsating flow state of blood in the artery; the second is that it cannot provide the vascular tissue with mechanical mechanisms such as periodic axial stretching, radial stretching, and twisting that approximate the physiological state at the same time. The third is that most of them use the method of no wall rotation, which needs to be improved in terms of uniform cell, high-density seeding and mass transfer.

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