The invention discloses a medicament used for intensive care unit sterilization and directed at a variety of pathogenic bacteria. The medicament is composed of the following raw materials by weight: 1-5 parts of alcohol, 4-8 parts of ethanol, 3-7 parts of coptis chinensis, 2-6 parts of phellodendron, 3-7 parts of Scutellaria baicalensis, 3-14 parts of Lonicera japonica, 1-6 parts of isatis root, 4-9 parts of forsythia, 2-9 parts of andrographis paniculata, 3-9 parts of houttuynia cordata, 2-8 parts of dandelion, 2-7 parts of Viola philippica, 2-7 parts of purslane, 3-8 parts of climbing groundsel herb, 2-10 parts of wild chrysanthemum, 2-7 parts of Eucalyptus robusta, 3-12 parts of radix bupleuri, 1-5 parts of Fructus Cnidii, 6-14 parts of folium artemisiae argyi, 7-12 parts of Sansevieria trifasciata, 6-10 parts of Indigofera bungeana herb and root, 1-3 parts of atractylodes lancea, 1-4 parts of Tricyrtis pilosa Wall, 5-11 parts of Acorus calamus, and 2-8 parts of mint leaf. According to the medicament used for intensive care unit sterilization and directed at a variety of pathogenic bacteria and the preparation method thereof, along with people's increasing pursuit for green, the medicament can kill pathogenic bacteria entrained in crowds and air, also has the advantages of fresh smell, refreshing effect, low cost, good safety, and obvious disinfection effect, and is suitable for large-scale popularization.