Method for detecting somatic cell number in raw milk

A technology of somatic cells and raw milk, applied in the determination/inspection of microorganisms, biochemical equipment and methods, measuring devices, etc., can solve the problems of cumbersome operation, inaccuracy, high cost, etc. The effect of easy operation

Inactive Publication Date: 2009-03-11
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims to solve the problems that existing methods for detecting the number of somatic cells in raw milk are time-consuming, cumbersome to operate, require professional technicians to operate, or have high cost, or are not accurate enough, and provide a method for measuring the number of somatic cells in raw milk. Methods

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  • Method for detecting somatic cell number in raw milk
  • Method for detecting somatic cell number in raw milk

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specific Embodiment approach 1

[0013] Specific Embodiment 1: The method for determining the number of somatic cells in raw milk in this embodiment is carried out as follows: 1. Pretreatment: Take two parts of the raw milk to be tested, named as sample 1 and sample 2 respectively, and the somatic cells in sample 1 Filter out to obtain the reference substance without somatic cells. Take the equal volume of sample 2 and the reference substance and add them to a transparent container, and preheat to 36-38°C; 2. Addition of reaction reagents: add ester solution, buffered saline solution and diazonium salt solution, each 1mL of sample 2 and reference substance need to add 0.01-0.1mL of ester solution, 0.01-0.1mL of buffered saline solution and 0.01-0.1mL of diazonium salt solution; 3. Determination of color Change: Immediately use the instrument to collect the color information of sample 2 and the reference substance after adding the reaction reagent in step 2, and record the time corresponding to the color inform...

specific Embodiment approach 2

[0017] Embodiment 2: The difference between this embodiment and Embodiment 1 is that in Step 1, the sample 2 and the reference substance were preheated to 37° C. respectively. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0018] Embodiment 3: The difference between this embodiment and Embodiment 1 is that the ester in step 2 is naphthyl chloroacetate, indoxyl ester or pyrrolyl ester. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

A method for measuring the number of somatic cells in raw milk relates to a method for measuring the number of somatic cells in milk. The invention solves the problems of long time consuming, tedious operation and carrying out operation by professional technical personnel, or high lost, or inaccuracy existing in the existing method for measuring the number of somatic cells in raw milk. The method is carried out by following steps: firstly, pretreatment; secondly, addition of reactive reagent; thirdly, measurement of change in color; and finally the number of somatic cells in raw milk is obtained. The method has the advantages of low cost, time saving, simple and convenient operation and accurate measurement result.

Description

technical field [0001] The invention relates to a method for measuring the number of somatic cells in milk. Background technique [0002] The number of somatic cells in raw milk refers to the total number of cells per milliliter of raw milk. Most of the somatic cells are white blood cells, accounting for more than 95% of the total, and the rest are exfoliated epithelial cells. The somatic cell count is an important index to measure the quality of raw milk and the health of the mammary glands of animals supplying raw milk. A low-cost, high-accuracy method for the determination of total somatic cells in bovine milk. [0003] At present, there are many detection methods for bovine milk somatic cell count or mastitis used in the dairy industry at home and abroad. The commonly used methods include microscope detection (standard microscope method and fluorescence microscope method), electronic counter detection method, California cell number measurement method (CMT), Wisconsin m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/10G01N21/25C12Q1/02
Inventor 田波霍贵成姜瞻梅韩翠萍陈晓义史慧茹
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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