Method for producing animal model of essential thrombocytopenia

Abstract

The invention relates to a preparation method which comprises the following steps: taking a BALB / C mouse, extracting eye balls or sampling blood from an orbitall vein, carrying out EDTA-Na2 anticoagulation, separating a blood platelet in a gradient centrifugation manner and washing, and adjusting the concentration into 1 to 2*10 / L; taking the suspension liquid of the blood platelet to be respectively mixed with equivalent complete freund adjuvant and incomplete freund adjuvant to form a milk white oil pocket water-shaped admixture which is used as antigen liquid; taking the antigen liquid containing the complete freund adjuvant to be respectively injected into the rear foot palm, the back and the groin of a guinea pig for four times, wherein each injection contains at least 5 points and the injection amount of each point is 100 microlitres; taking the whole blood of the guinea pig at the sixth week and taking blood serum for standby after the whole blood is centrifuged; taking the antiserum from -20 DEG C and placing the antiserum after being melted completely into water bath of 56 DEG C for 30 min; injecting the antiserum to the abdominal cavity of the BALB / C mouse on the first, the third, the fifth, the seventh, the ninth, the eleventh and the thirteen days and taking out on the fifteenth days, wherein the injection amount of each time is 100 microlitres. The invention solves the problem of shorter maintenance time of the blood platelet. The stable and controllable animal model of the idiopathic thrombocytopenic purpura of the mouse can be prepared.

Description

Technical field: [0001] The invention relates to a method for making animal models of idiopathic thrombocytopenic purpura, which can be used for making animal models of idiopathic thrombocytopenic purpura in mice. Background technique: [0002] At present, domestic experimental research on idiopathic thrombocytopenic purpura (ITP) mainly adopts immunization method to establish animal models, or adopts immunization method combined with other methods to establish models. The reasons why this modeling method is widely used: 1) The antigen preparation process is simple and the modeling rate is high. 2) ITP is highly similar to human ITP in terms of pathological features and healing mechanism. 3) The ITP model is highly sensitive to various anti-ITP drugs. This model has become the standard model for monitoring potential anti-ITP compounds. However, the significant problem of this traditional modeling method is that the degree of thrombocytopenia is not easy to grasp, and the ...

AI Technical Summary

Problems solved by technology

However, the significant problem of this traditional modeling method is that the degree of thrombocytopenia is not easy to grasp, and the maintenance time of thrombocytopenia is short. It is necessary to investigate the dosage of mouse platelet antiserum (APS), the interval time of serum injection and the degree and degree of thrombocytopenia. Maintain the relationship between the length of time to ensure the success rate of animal models, which is conducive to determining the best time point for treatment and drug effects