Correct measurement method for nucleus DNA matter content in cell quantitative investigation

Abstract

The invention provides a method for accurately measuring the content of karyon DNA substances in quantitative analysis of cells, including the following steps: expansion algorithm mathematical morphology is used for obtaining EIOD value; statistical method is used for obtaining EIOD standard value so that the IOD correction coefficient of a single karyon is calculated; the characteristic parameters and correction coefficient of karyon are used for training the regression model of the correction coefficient by SVR algorithm, and the model and the SVR algorithm are used for returning and reconstructing CIOD value of cells; the CIOD value of reference karyon is used for converting the content of DNA substances of each karyon. The method improves the accuracy and anti-interference of measuring the content of DNA substances; the conversion method of the content of DNA substances in the invention can obtain the content of DNA substances which can be easily understood and utilized, is the same as medical expression, and is conveniently applied to diagnosis or research.

Description

technical field [0001] The invention relates to an accurate measurement method for the content of cell nucleus DNA in quantitative analysis of cells, belonging to the field of biomedical engineering. Background technique [0002] The determination of nuclear DNA content is the core of quantitative cell analysis, and it is also the necessary prerequisite and the most important basis for using quantitative cell analysis methods to diagnose and screen early cancers and to study the relationship between changes in nuclear DNA content and various lesions. The accuracy of DNA content measurement is directly related to the accuracy and stability of cancer screening, and also directly affects the authenticity of quantitative cytology research results, which is of great significance. [0003] Generally, the DNA content of the nucleus cannot be directly measured, but the optical absorption of the DNA material in the nucleus can be measured by measuring the integrated optical density v...

AI Technical Summary

Problems solved by technology

[0005] In patents US 6,026,174 and US 7,274,908, the IOD value of the nucleus is used to calculate the DNA content, and the corresponding DNA content calculation method is given, but the corresponding correction method is not given

In the actual application process, the calculation of DNA content is easily affected by various objective factors outside, making the calculated DNA content value inaccurate, thus affecting the accuracy of tumor diagnosis

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