Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Correct measurement method for nucleus DNA matter content in cell quantitative investigation

A quantitative analysis and material content technology, applied in the field of biomedical engineering, can solve problems that affect the accuracy of tumor diagnosis, no correction method, inaccurate DNA content, etc., to achieve easy understanding and use, improve anti-interference, The effect of improving accuracy

Inactive Publication Date: 2009-07-29
WUHAN LANDING INTELLIGENCE MEDICAL CO LTD
View PDF2 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In patents US 6,026,174 and US 7,274,908, the IOD value of the nucleus is used to calculate the DNA content, and the corresponding DNA content calculation method is given, but the corresponding correction method is not given
In the actual application process, the calculation of DNA content is easily affected by various objective factors outside, making the calculated DNA content value inaccurate, thus affecting the accuracy of tumor diagnosis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Correct measurement method for nucleus DNA matter content in cell quantitative investigation
  • Correct measurement method for nucleus DNA matter content in cell quantitative investigation
  • Correct measurement method for nucleus DNA matter content in cell quantitative investigation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0013] The present invention will solve the following problems:

[0014] (1) Provide a method for accurately measuring the content of DNA material in the nucleus, improve the accuracy of measuring the content of DNA material in the nucleus, prevent interference, and the like.

[0015] (2) Provide a method for correcting the IOD value, so that the corrected IOD value can more accurately reflect the amount of DNA material in the nucleus.

[0016] (3) An accurate conversion method for the DNA content of the cell nucleus can be provided, and the converted DNA content value can provide comparability of the DNA content of different cell nuclei within the sample.

[0017] The specific implementation of the embodiment of the present invention is as follows: it mainly includes three aspects: calculating the extended integral optical density (EIOD), correcting t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for accurately measuring the content of karyon DNA substances in quantitative analysis of cells, including the following steps: expansion algorithm mathematical morphology is used for obtaining EIOD value; statistical method is used for obtaining EIOD standard value so that the IOD correction coefficient of a single karyon is calculated; the characteristic parameters and correction coefficient of karyon are used for training the regression model of the correction coefficient by SVR algorithm, and the model and the SVR algorithm are used for returning and reconstructing CIOD value of cells; the CIOD value of reference karyon is used for converting the content of DNA substances of each karyon. The method improves the accuracy and anti-interference of measuring the content of DNA substances; the conversion method of the content of DNA substances in the invention can obtain the content of DNA substances which can be easily understood and utilized, is the same as medical expression, and is conveniently applied to diagnosis or research.

Description

technical field [0001] The invention relates to an accurate measurement method for the content of cell nucleus DNA in quantitative analysis of cells, belonging to the field of biomedical engineering. Background technique [0002] The determination of nuclear DNA content is the core of quantitative cell analysis, and it is also the necessary prerequisite and the most important basis for using quantitative cell analysis methods to diagnose and screen early cancers and to study the relationship between changes in nuclear DNA content and various lesions. The accuracy of DNA content measurement is directly related to the accuracy and stability of cancer screening, and also directly affects the authenticity of quantitative cytology research results, which is of great significance. [0003] Generally, the DNA content of the nucleus cannot be directly measured, but the optical absorption of the DNA material in the nucleus can be measured by measuring the integrated optical density v...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N21/27G06T7/00G06K9/00
Inventor 庞宝川徐端全
Owner WUHAN LANDING INTELLIGENCE MEDICAL CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com