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Artificial cultivation method for sclerotium and fruiting body of Inonotus obliquus

A technology of Inonotus obliquus and artificial cultivation, which is applied in botanical equipment and methods, fertilizer mixtures, horticulture and other directions, and can solve the problems of little progress in artificial domestication and cultivation of Inonotus obliquus, low yield of sclerotia and fruiting bodies, and bacterial growth. The problem of low efficiency of nuclear biology, etc., can achieve the effect of large quantity, neat and dense mycelium, and good quality.

Inactive Publication Date: 2010-08-18
BEIJING FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the increasing market demand, the wild resources are increasingly depleted and cannot meet the growing market demand. Therefore, it is necessary to conduct strain isolation and artificial domestication and cultivation research on wild Inonotus obliquus
[0004] There have been many reports on the medicinal value and active ingredients of Inonotus obliquus at home and abroad, but there is little progress in the research on the artificial domestication and cultivation of Inonotus obliquus
So far, the existing research on the artificial domestication and cultivation of Inonotus obliquus has mostly stayed at the level of a single factor that affects the growth of the sclerotia or fruiting body of Inonotus obliquus, such as light, culture temperature, relative air humidity, or medium. The effect of a single factor in the formula composition on the growth of Inonotus obliquus sclerotia or fruiting body; because the above factors are connected and interact with each other, they jointly affect the growth and quality of Inonotus obliquus sclerotia or fruiting body , only considering the growth of Inonotus obliquus sclerotia or fruiting bodies by a single factor, the existing methods of artificial domestication and cultivation of Inonotus obliquus mostly have slow mycelial growth rate and low sclerotia biological efficiency, Defects such as low yield of sclerotia and fruiting body

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1), the parent species of Inonotus obliquus isolated from the sclerotia of wild Inonotus obliquus is enriched in PDA medium (glucose 20g, agar 20g, soybean powder 10g, KH 2 PO 4 1g, M g SO 4 0.5g, 1000ml water, natural pH. ) after expanded culture, inoculated on the original seed culture medium (dry matter composition: 98% of corn kernels, 2% of calcium carbonate; 65% water content in the final culture medium) and cultivated to obtain the original species of Inonotus obliquus;

[0022] (2), Inonotus obliquus original species is inoculated in the cultivar culture medium in the cultivation bag (dry matter composition: birch sawdust 52%, corncob 26%, wheat bran 20%, white sugar 1%, gypsum 1%; Final The water content in the culture medium is 65%;) cultivated in a constant temperature darkroom at 25°C, and the humidity in the cultivation chamber is natural humidity; the average daily growth rate of Inonotus obliquus mycelium is 0.43mm / d;

[0023] (3) After the mycelium ...

Embodiment 2

[0027] The composition of the cultivar medium in this embodiment is: 50% of birch sawdust, 28% of corn cob, 18% of wheat bran, 2% of bean cake powder, 1% of white sugar, and 1% of gypsum; the water content of the medium is 60%. Except that, all the other are identical with embodiment 1;

[0028] Test results: in step (2): the average daily growth rate of Inonotus obliquus mycelia is 0.67mm / d; in step (3): about 42 days after the formation of the primordia, the sclerotia and fruiting body begin to become hard, the color From shallow to dark; the biological efficiency of sclerotia is 37%.

Embodiment 3

[0030] In this embodiment, in step (3), when the sclerotia and fruiting bodies start to break through the cultivation bag wall, immediately cover a cultivation bag on the periphery or stick the bursting position with adhesive tape to increase the CO in the bag. 2 Concentration; Except this, all the other are identical with embodiment 2;

[0031] Test results: in step (2): the average daily growth rate of Inonotus obliquus mycelium is 0.67mm / d; in step (3): about 38 days after the formation of the primordia, the sclerotia and fruiting body begin to become hard, the color From shallow to dark; the biological efficiency of sclerotia is 67%.

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Abstract

The present invention discloses an artificial cultivation method of sclerotium and sporophore of innotus obliquus, wherein the method comprises the following steps: after executing expanding culture to the mother seed of innotus obliquus on the enriched PDA culture medium for obtaining the stock seed; inoculating the stock seed in the cultivated species culture medium for cultivating unit the mycelium is overgrown. The culture bag which is overgrown with mycelium is transferred to a mushroom generating room for mushroom generating culture, wherein the mushroom generating culture is executed below the following conditions: relative air moisture of air for 80%, culture temperature for 25 DEG C, and illumination intensity for 1001x. The artificial cultivation method of the invention can remarkably increase the growing speed of mycelium of sclerotium. The average growth rate of mycelium in one day can obtain 0.67mm / d furthest, and the growth of mycelium is tidy and compact. When the bag is filled with mycelium, the budding time of fungus bag is early. The number of generated sclerotium and sporophore is large. The production volume is high and the quality is excellent, wherein, the biological efficiency of sclerotium reaches up to 67%.

Description

technical field [0001] The invention relates to an artificial cultivation method of medicinal fungi, in particular to an artificial cultivation method of Inonotus obliquus sclerotia and fruiting body, and belongs to the field of artificial cultivation of medicinal fungi. Background technique [0002] Inonotus obliquus (Pers.: Fr.) Pilát, also known as Inonotus obliquus (Pers.: Fr.) Pilát, commonly known as chaga, birch tea, tea can, belongs to Basidiomycota and Hymenomycetes , Aphyllophorales, Hymenochaetaceae, and Inonotus are very precious medicinal fungi. Fruiting body of Inonotus obliquus: Basidiocarp is annual, flat, adnate, not easy to separate from the substrate, usually grows under the bark, corky to hardwood when fresh, odorless and tasteless, hardwood when dry. The flat surface can be up to 20cm long, 7cm wide, and 5mm thick; the sterile margin is narrow or wide, light yellow, creamy yellow when actively growing, and 5mm wide. The surface of the orifice is brown ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04C05G3/00
Inventor 陈艳秋戴玉成崔宝凯杜萍
Owner BEIJING FORESTRY UNIVERSITY