Method for culturing cyprids of balanus reticulates on large scale

Abstract

The invention discloses a method for culturing cyprids of balanus reticulates on a large scale, which comprises the following steps: after ovum of the balanus reticulates is bred into naupliar larva, putting the naupliar larva into a culture medium with bait for constant temperature culture in dark environment; performing intermittent lighting on the naupliar larva in the constant temperature culture process; and performing cold storage on the appeared cyprids. The method can overcome the defects of high death rate, small and unstable yield, quick settlement and metamorphosis processes, incapacity of storage and accumulation and the like in the breeding process of the cyprids, greatly improve the yield of the cyprids, effectively prolong the time of a drifting stage of the cyprids, promote the settlement synchrony of the cyprids, and provide required experimental materials for screening marine fouling organism antifouling compositions and studying environmental toxicology, along with wide application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for cultivating netted barnacle Venus larvae in large quantities. Background technique [0002] Marine fouling organisms are the general term for animals, plants and microorganisms that inhabit or attach to ships and various underwater artificial facilities in the marine environment, adversely affect human economic activities, and bring negative benefits to investors . The attachment of fouling organisms to the ship will reduce the speed of the ship, reduce the inner diameter of the pipeline, change the metal corrosion process, and even cause the navigation mark to shift; the attachment to the aquaculture facility will occupy the effective attachment surface of some aquaculture objects, hindering the net cage. The exchange of internal and external water bodies affects the growth and development of the cultured objects; and some species (such as barnacles) have strong adhesion...

AI Technical Summary

Problems solved by technology

[0007] Prior art has disclosed the cultivation method about grain barnacle, but (1) this kind of organism is different with net grain barnacle, and it is mainly the dominant species of fouling organism in northern sea area, and net grain barnacle is tropical subtropical warm water species, It is a dominant species of representative fouling organisms in the coastal sea area of ​​South China; (2) Fresh seawater needs to be replaced every 24 hours in the process of cultivating barnacles, which increases the workload; (3) the bait for cultivating barnacles is Scleralis sp. (Platymonas helgolandica Kylin var.tsingtaoensis Tseng et T.J.Chang var.nov.); (4) The cultivation is carried out under the condition of no light, which will easily cause the algae as bait to sink to the bottom and hinder the feeding of the larvae; (5) This method does not Discuss the method of storing Venus larvae for backup. The obtained Venus larvae must be used in research and testing in time, otherwise, Venus larvae will soon appear metamorphosis; For statistical analysis, usually only 2 parallel replicate samples are set; however, currently widely used biostatistical analysis methods require that each group (including blank group, control group, and experimental treatment group) need to set up 3 to 5 parallel replicate samples, each parallel replicate The number of larvae in the sample is about 30, which leads to a significant increase in the number of larvae required for testing than in the past. The number of larvae obtained in one batch of culture may not meet the number required for the test experiment.

Therefore prior art does not solve the technical scheme of reticulated barnacle cultivation and storage, and reticulated barnacle and pattern barnacle are two different species, and the method for cultivating pattern barnacle larva is also not suitable for the growth of reticulated barnacle larvae. nourish