Method for culturing cyprids of balanus reticulates on large scale

A technology of reticulated barnacles and Venus larvae, applied in the direction of animal husbandry, etc., can solve the problems of no technical solutions for the cultivation and storage of reticulated barnacles, unsuitable cultivation of reticulated barnacle larvae, and hindrance of larvae feeding, etc., to achieve The effect of strong adhesion synchronization, promotion of synchronization, and long storage period

Inactive Publication Date: 2009-11-18
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
View PDF0 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Prior art has disclosed the cultivation method about grain barnacle, but (1) this kind of organism is different with net grain barnacle, and it is mainly the dominant species of fouling organism in northern sea area, and net grain barnacle is tropical subtropical warm water species, It is a dominant species of representative fouling organisms in the coastal sea area of ​​South China; (2) Fresh seawater needs to be replaced every 24 hours in the process of cultivating barnacles, which increases the workload; (3) the bait for cultivating barnacles is Scleralis sp. (Platymonas helgolandica Kylin var.tsingtaoensis Tseng et T.J.Chang var.nov.); (4) The cultivation is carried out under the condition of no light, which will easily cause the algae as bait to sink to the bottom and hinder the feeding of the larvae; (5) This method does not Discuss the method of storing Venus larvae for backup. The obtained Venus larvae must be used in research

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0025] Example 1

[0026] The culture medium is natural seawater, and the seawater is filtered, boiled and sterilized, cooled to room temperature, and placed in a high-temperature sterilized vessel for later use.

[0027] Collect mature individuals of reticulated barnacles, select larger individuals when sampling, and their appearance is intact.

[0028] Adult barnacles were dissected, and mature fertilized egg masses were selected and placed in a 500ml beaker filled with sterile seawater for incubation. (The 500ml beaker used in this example refers to the beaker used for collecting barnacle egg masses and hatching nauplius, because the beaker is too large. It is easy to operate, but the beaker is too small and there is little water body, which is not conducive to separating the unhatched egg mass and the hatched nauplius, the same below), and then move the collected nauplius into a 1000ml beaker for cultivation according to the following steps:

[0029] (1) After the eggs of the ...

Example Embodiment

[0034] Example 2

[0035] The culture medium is natural seawater, and the seawater is filtered, boiled and sterilized, cooled to room temperature, and placed in a high-temperature sterilized vessel for later use.

[0036] Collect mature individuals of reticulated barnacles, select larger individuals when sampling, and their appearance is intact.

[0037] The adult barnacles were dissected, the mature fertilized egg masses were selected and incubated in a 500ml beaker filled with sterile seawater, and then the collected nauplii larvae were cultured according to the following steps:

[0038] (1) After the eggs of the reticulated barnacle hatch into nauplius, use seawater as the culture medium (1000ml), and the subcordiform algae as the bait (2.5~3.0×105 Cells / ml), cultured in a dark environment at room temperature (25~28℃); the density of larvae in seawater is 1~2 larvae / ml;

[0039] (2) The beaker containing barnacle larvae is exposed to natural light for 1 hour every morning and e...

Example Embodiment

[0043] Example 3

[0044] The culture medium is natural seawater, and the seawater is filtered, boiled and sterilized, cooled to room temperature, and placed in a high-temperature sterilized vessel for later use.

[0045] Collect mature individuals of reticulated barnacles, select larger individuals when sampling, and their appearance is intact.

[0046] The adult barnacles were dissected, the mature fertilized egg masses were selected and incubated in a 500ml beaker filled with sterile seawater, and then the collected nauplius larvae were cultured in groups according to the following steps:

[0047] (1) Divide into four groups for implementation. In the early stage, after the eggs of the reticulated barnacle hatch into nauplius, use 1000ml of seawater as the culture medium, and the subcordiform algae as the bait, and put the nauplius in the incubator Cultivate in a dark environment at a constant temperature of 29~31℃; the density of larvae in seawater is 2 / ml;

[0048] The differ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for culturing cyprids of balanus reticulates on a large scale, which comprises the following steps: after ovum of the balanus reticulates is bred into naupliar larva, putting the naupliar larva into a culture medium with bait for constant temperature culture in dark environment; performing intermittent lighting on the naupliar larva in the constant temperature culture process; and performing cold storage on the appeared cyprids. The method can overcome the defects of high death rate, small and unstable yield, quick settlement and metamorphosis processes, incapacity of storage and accumulation and the like in the breeding process of the cyprids, greatly improve the yield of the cyprids, effectively prolong the time of a drifting stage of the cyprids, promote the settlement synchrony of the cyprids, and provide required experimental materials for screening marine fouling organism antifouling compositions and studying environmental toxicology, along with wide application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for cultivating netted barnacle Venus larvae in large quantities. Background technique [0002] Marine fouling organisms are the general term for animals, plants and microorganisms that inhabit or attach to ships and various underwater artificial facilities in the marine environment, adversely affect human economic activities, and bring negative benefits to investors . The attachment of fouling organisms to the ship will reduce the speed of the ship, reduce the inner diameter of the pipeline, change the metal corrosion process, and even cause the navigation mark to shift; the attachment to the aquaculture facility will occupy the effective attachment surface of some aquaculture objects, hindering the net cage. The exchange of internal and external water bodies affects the growth and development of the cultured objects; and some species (such as barnacles) have strong adhesion...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01K67/033
Inventor 严涛曹文浩严文侠董钰
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap