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Method for inducing differentiation of olfactory stem cells by olfactory ensheathing cell culture supernatant

A technology for stem cell differentiation and olfactory ensheathing cells, which is applied in the field of olfactory ensheathing cell culture supernatant to induce olfactory stem cell differentiation, which can solve the problem that the research on olfactory stem cell differentiation is rarely reported.

Inactive Publication Date: 2009-12-02
BEIJING HONGTIANJI NEUROSCI ACADEMY
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Problems solved by technology

[0002] It has been reported that different stem cells are induced to differentiate in vitro, and cell transplantation is attempted after in vitro induced differentiation, but there are few reports on the induced differentiation of olfactory stem cells

Method used

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Examples

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Embodiment Construction

[0006] (1) Collect fetuses without obvious developmental disorder from abortion and induced labor in obstetrics and gynecology department, separate and obtain olfactory ensheathing cells and primary olfactory neural stem cells respectively.

[0007] (2) Reagents Medium (DMEM / F12), cytokines (B27), epidermal growth factor (EGF), fibroblast growth factor (FGF), glutamine, collagenase.

[0008] (3) Craniotomy along the brow arch of the dead fetus, remove the brain tissue, expose the anterior skull base, remove the olfactory bulb to see the cribriform plate and cockscomb, cut the skull base bone plate around the cribriform plate, and cut the lateral walls of the nasal cavity on both sides The upper and middle turbinates and the corresponding middle and upper parts of the nasal septum were removed and put into the culture medium to wash several times to wash away the blood on the olfactory mucosa. Transfer the crushed tissue into a 15ml centrifuge tube, add 5ml of basal medium, blo...

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Abstract

The invention relates to a method for inducing differentiation of olfactory stem cells by olfactory ensheathing cell culture supernatant, which comprises the following steps: collecting fetuses who are aborted and laboured in obstetrics and gynecology department and have no obvious maldevelopment, and respectively isolating and acquiring olfactory ensheathing cells and primary olfactory neural stem cells. After inducing differentiation in vitro, that human embryo olfactory stem cells are applied to clinical cell transplantation because of central nervous system lesions is ensured to become possible.

Description

Technical field [0001] The patent of the present invention relates to a method for inducing the differentiation of olfactory stem cells by culture supernatant of olfactory ensheathing cells, and obtaining mature nerve cells after differentiation of olfactory stem cells, which are used for the clinical treatment of central nervous system diseases or damaged cell transplantation. Background technique [0002] It has been reported that different stem cells have been induced to differentiate in vitro, and cell transplantation after in vitro induced differentiation has been attempted, but there are few reports on the induced differentiation of olfactory stem cells. Contents of the invention [0003] In order to overcome the difficulties in clinical treatment of central nervous system lesions or damaged cell transplantation, the patent of the present invention provides a method for inducing olfactory stem cell differentiation with olfactory ensheathing cell culture supernatant. ...

Claims

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Application Information

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IPC IPC(8): C12N5/08A61K35/54A61P25/00A61K35/30
Inventor 黄红云包建玲王洪美张秀东
Owner BEIJING HONGTIANJI NEUROSCI ACADEMY
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