Method for inducing differentiation of olfactory stem cells by olfactory ensheathing cell culture supernatant

A technology for stem cell differentiation and olfactory ensheathing cells, which is applied in the field of olfactory ensheathing cell culture supernatant to induce olfactory stem cell differentiation, which can solve the problem that the research on olfactory stem cell differentiation is rarely reported.

Inactive Publication Date: 2009-12-02
BEIJING HONGTIANJI NEUROSCI ACADEMY
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] It has been reported that different stem cells are induced to differentiate in vitro, and cell transplantation is atte

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0006] (1) Collect fetuses without obvious developmental disorder from abortion and induced labor in obstetrics and gynecology department, separate and obtain olfactory ensheathing cells and primary olfactory neural stem cells respectively.

[0007] (2) Reagents Medium (DMEM / F12), cytokines (B27), epidermal growth factor (EGF), fibroblast growth factor (FGF), glutamine, collagenase.

[0008] (3) Craniotomy along the brow arch of the dead fetus, remove the brain tissue, expose the anterior skull base, remove the olfactory bulb to see the cribriform plate and cockscomb, cut the skull base bone plate around the cribriform plate, and cut the lateral walls of the nasal cavity on both sides The upper and middle turbinates and the corresponding middle and upper parts of the nasal septum were removed and put into the culture medium to wash several times to wash away the blood on the olfactory mucosa. Transfer the crushed tissue into a 15ml centrifuge tube, add 5ml of basal medium, blo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for inducing differentiation of olfactory stem cells by olfactory ensheathing cell culture supernatant, which comprises the following steps: collecting fetuses who are aborted and laboured in obstetrics and gynecology department and have no obvious maldevelopment, and respectively isolating and acquiring olfactory ensheathing cells and primary olfactory neural stem cells. After inducing differentiation in vitro, that human embryo olfactory stem cells are applied to clinical cell transplantation because of central nervous system lesions is ensured to become possible.

Description

Technical field [0001] The patent of the present invention relates to a method for inducing the differentiation of olfactory stem cells by culture supernatant of olfactory ensheathing cells, and obtaining mature nerve cells after differentiation of olfactory stem cells, which are used for the clinical treatment of central nervous system diseases or damaged cell transplantation. Background technique [0002] It has been reported that different stem cells have been induced to differentiate in vitro, and cell transplantation after in vitro induced differentiation has been attempted, but there are few reports on the induced differentiation of olfactory stem cells. Contents of the invention [0003] In order to overcome the difficulties in clinical treatment of central nervous system lesions or damaged cell transplantation, the patent of the present invention provides a method for inducing olfactory stem cell differentiation with olfactory ensheathing cell culture supernatant. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/08A61K35/54A61P25/00A61K35/30
Inventor 黄红云包建玲王洪美张秀东
Owner BEIJING HONGTIANJI NEUROSCI ACADEMY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap