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Vitro rapid propagation method of kefir lily by using tender petals as explant

A technology for explants and Clivia, which is applied to the field of asexual and rapid propagation of flowers, can solve problems such as limiting the application of Clivia tissue culture technology, and achieve the effects of efficient and rapid reproduction, low variation, and high-efficiency regeneration system.

Inactive Publication Date: 2012-05-09
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This limits the application of Clivia tissue culture technology in actual production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] 1. Explant inoculation and callus induction: the development degree of the young inflorescences obtained is: the size of the flower buds has been or is nearly fully developed but is still green. Put it into a sterile ultra-clean bench, soak it with 75% (volume / volume) alcohol for 30 seconds, soak it with 0.1% (mass / volume) mercuric chloride solution for 10 minutes, and rinse it with sterile water three times. Cut the bud from the uppermost part of the ovary, then cut off most of the upper part of the bud, cut the remaining middle part longitudinally twice, and inoculate it in a medium containing 6-BA0.2mg / L, 2,4-D0.1mg / L, 3% sucrose 40W fluorescent lamp was irradiated for 2 hours per day at 20°C on MS medium. Subculture once every 30 days, and induce differentiation when the yellow-green callus grows.

[0013] 2. Induction of adventitious buds: transfer the yellow-green callus to the induction differentiation medium: MS+6-BA0.5mg / L+2, 4-D0.1+3% sucrose, subculture once...

Embodiment 2

[0017] 1. Explant inoculation and callus induction: The development degree of the young inflorescences obtained is: the size of the flower buds has been or is nearly fully developed but is still green. The flower buds of this development degree are cut from the lower end of the ovary and removed. Cut it open, put it into a sterile ultra-clean bench, soak it with 75% (volume / volume) alcohol for 60 seconds, soak it with 0.2% (mass / volume) mercuric chloride solution for 3 minutes, and rinse it with sterile water 5 times. Cut the bud from the uppermost part of the ovary, then cut off most of the upper part of the bud, cut the remaining middle part longitudinally twice, and inoculate it in a medium containing 6-BA0.2mg / L, 2,4-D0.5mg / L, 3.5% sucrose MS culture medium, irradiated with 40W fluorescent lamp for 4 hours per day at 28°C. Subculture once every 50 days, and induce differentiation when the yellow-green callus grows.

[0018] 2. Induction of adventitious buds: transfer the ...

Embodiment 3

[0022] 1. Explant inoculation and callus induction: The development degree of the young inflorescences obtained is: the size of the flower buds has been or is nearly fully developed but is still green. The flower buds of this development degree are cut from the lower end of the ovary and removed. Cut it open, put it into a sterile ultra-clean bench, soak it with 75% (volume / volume) alcohol for 45 seconds, soak it with 0.15% (mass / volume) mercuric chloride solution for 8 minutes, and rinse it with sterile water 4 times. Cut the bud from the uppermost part of the ovary, then cut off most of the upper part of the bud, cut the remaining middle part longitudinally twice, and inoculate it in a medium containing 6-BA0.2mg / L, 2,4-D0.3mg / L, 3.2% sucrose MS culture medium, irradiated with 40W fluorescent lamp for 3 hours per day at 25°C. Subculture once every 45 days, and induce differentiation when the yellow-green callus grows.

[0023] 2. Induction of adventitious buds: transfer the...

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PUM

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Abstract

The invention belongs to a flower propagation method, particularly relating to an asexual rapid propagation method of flowers. In the method, technologies of explant inoculation, callus induction, adventitious bud induction, root induction, regeneration plant transplantation are used, so that kefir lilies can generate large quantities of regeneration plants having the entirely same gene background and unified plant type within a short time. Petal bases of seedling inflorescence of clivia miniata are used as explants, and the dedifferentiation ratio and the differentiation ratio can both be more than 80%. Calluses induced by one explant can be differentiated into 700 cluster buds having the unified type through repeated regeneration; the rooting rate of the cluster buds can reach 100%, andthe survival rate of transplantation in favorable environment can reach 100%.

Description

technical field [0001] The invention belongs to flower propagation method, in particular to the asexual rapid propagation method of flower. Background technique [0002] Plant tissue culture is plant aseptic culture technology, which is based on the totipotency of plant cells, using isolated plant organs (such as roots, stems, leaves, stem tips, flowers, fruits, etc.), tissues (such as cambium, epidermis, Cortex, endosperm, etc.) or cells (such as megaspores, microspores, somatic cells, etc.) and protoplasts can induce callus, adventitious buds under sterile and suitable artificial medium and artificial conditions such as light and temperature. , adventitious roots, forming whole plants or techniques for producing other products of economic value. The regenerated plants produced by plant tissue culture can basically maintain the excellent traits of the parents (explant source plants) except for extremely low variation. Since the invention of plant tissue culture technology...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王丽王钦美
Owner NORTHEAST NORMAL UNIVERSITY
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