In Vitro Rapid Propagation Method of Dieffenbachia Tiger Eye Using Young Flowers as Explants
An explant, evergreen technology, applied in the directions of botanical equipment and methods, horticultural methods, plant regeneration, etc., can solve problems such as unfavorable subculture, restricting the application of tiger eye evergreen tissue culture technology, low reproduction coefficient, etc. Low, neat appearance, efficient regeneration system
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0012] 1. Explant inoculation and callus induction: When the size of the flower buds has been or nearly fully developed but is still green, cut off the flower buds on the young inflorescence from the lower end of the ovary, and put the complete flower buds into a sterile ultra-clean In Taichung, soak in 75% (volume / volume) alcohol for 60 seconds, soak in 0.1 (mass / volume) mercury chloride solution for 10 minutes, and rinse with sterile water 5 times. The bud is cut from the upper part of the ovary, then most of the upper part of the bud is cut off, and the remaining part is cut longitudinally twice, and inoculated in a medium containing 6-BA2.0mg / L, NAA0.1mg / L, sucrose 4% (mass / volume) , on MS medium with a pH value of 6.2, irradiated with fluorescent light for 4 to 6 hours a day at 20°C to 30°C, and the light intensity is 1500 to 2000 Lux. Subculture once every 25-35 days, and induce differentiation when the yellow-green callus grows.
[0013] 2. Induction of adventitious bu...
Embodiment 2
[0017] 1. Explant inoculation and callus induction: When the size of the flower buds has been or nearly fully developed but is still green, cut off the flower buds on the young inflorescence from the lower end of the ovary, and put the complete flower buds into a sterile ultra-clean In Taichung, soak in 75% (volume / volume) alcohol for 90 seconds, soak in 0.2% (mass / volume) mercury chloride solution for 8 minutes, and rinse with sterile water 5 times. The flower bud is cut off from the upper part of the ovary, then most of the upper part of the flower bud is cut off, the remaining part is cut longitudinally, and inoculated in a medium containing 6-BA1.5mg / L, NAA0.15mg / L, sucrose 3.5% (mass / volume), On MS medium with a pH value of 5.9, irradiate with fluorescent lamp for 4 to 6 hours every day at 20°C to 30°C, and the light intensity is 1500 to 2000 Lux. Subculture once every 25-35 days, and induce differentiation when the yellow-green callus grows.
[0018] 2. Induction of adv...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com