Preparation method of gardenia extract

Abstract

The invention provides a preparation method of a gardenia extract, which combines the adoption of activated carbon and separation of macroporous resin and comprises the following steps: first washing activated carbon of which the usage amount is 1-3 times with water, and then eluting the activated carbon with 50%-80% ethanol, wherein the eluting flow rate is 0.6-1 time of crude drug amount/hour; collecting and concentrating the ethanol eluent to be put on a macroporous resin column, wherein the sampling volume is 1-3 times of column volume; adsorbing at the flow rate of 0.2-1.0 time of columnvolume/hour; eluting with 1-3 times of column volume of water and 3%-10% ethanol with 1-3 times of column volume; eluting with 20%-50% ethanol with 3-8 times of column volume at the eluting flow rateof 0.2-0.5 time of column volume/hour; and collecting 20%-50% ethanol eluent. The invention has high extraction efficiency and low cost and is suitable for industrialized preparation.

Description

technical field [0001] The invention relates to a preparation method of a traditional Chinese medicine extract, in particular to a preparation method of a gardenia extract. Background technique [0002] At present, the extraction method of geniposide generally uses organic solvents such as chloroform and absolute ethanol to extract in a Soxhlet extraction bottle to obtain the total active ingredient geniposide in gardenia, and then separate it on a silica gel column. methanol, chloroform mixed solution elution, the eluent is recrystallized in acetone to obtain geniposide crystals, generally about 4.0 g of geniposide can be separated from 100 g of gardenia fruit, but this method is not suitable for industrial production. Only experimental production is possible. [0003] According to current literature reports, in the gardenia jasminoides extraction method with higher geniposide content, ethanol reflux extraction is often used, but ethanol reflux extraction has limitations, ...

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Problems solved by technology

Another document reports that geniposide is extracted by microwave. From the data of geniposide yield, the yield of microwave method and boiling method has not been significantly improved, only 4 percentage points higher, but the cost of microwave extraction equipment is high. not widely used

Cai Meiqiang, Ji Jianbing, etc. studied the ultrasonic extraction method, but this extraction method is not suitable for large-scale production, and the equipment is expensive

[0004] However, it has been reported in the literature that alumina column chromatography has a good effect on the separation of geniposide, but the price of alumina is relatively high, and its application in production has certain limitations.

Another literature report uses macroporous resin alone to purify geniposide. The extraction methods of higher purity geniposide and iridoid glycosides obtained by this method are all extracted with ethanol. For example, Yao Qian et al. use HPD450 macroporous resin to adsorb and purify The total iridoid glycosides and geniposides of the fruit, the geniposide content in the obtained extract is 62.28%, and the total iridoid glycoside content is 83.72%. The extraction process is to take the gardenia medicinal material, crush it, weigh 20kg, Add 8 times the amount of 70% ethanol, reflux twice, each time for 1.5h, combine the extract, high-speed centrifugation, recover ethanol from the centrif

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