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Method for extracting oxidation resistant water-soluble matter of abalone shell

A technology of water-soluble substances and extraction methods, which is applied in the fields of water-soluble polysaccharide extraction and abalone shell waste utilization, can solve the problems of medicinal use or direct discarding, low utilization rate, etc., and achieve low cost, easy extraction, and high removal of superoxide free The effect of base activity

Inactive Publication Date: 2010-06-09
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide an extraction method of waste abalone shells with antioxidant water-soluble polysaccharides; to solve the problem in the prior art that the treatment of abalone shells is generally for medicinal use after grinding or directly discarded, and the utilization rate is low. Simple, the extract has natural antioxidant activity and can be made into antioxidants; abalone shell is rich in resources, easy to extract, and has potential for development

Method used

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  • Method for extracting oxidation resistant water-soluble matter of abalone shell
  • Method for extracting oxidation resistant water-soluble matter of abalone shell
  • Method for extracting oxidation resistant water-soluble matter of abalone shell

Examples

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Effect test

Embodiment 1

[0026] Preparation and purification of abalone shell water-soluble matrix extract

[0027] Rinse the wrinkled abalone shells produced in Fujian, dry them naturally, and grind them into 60-120 mesh powder with a pulverizer. Weigh 3kg of powder into 6L of distilled water, stir and dissolve at 60°C for 2 hours, take out the mixture and filter it with suction, extract the residue twice with water and merge it into the mixture, remove the precipitated supernatant and freeze-dry it into a powder. The powder is the water-soluble matrix of the abalone shell, which is 0.3g in total.

[0028] Dissolve 1 g of freeze-dried powder in 50 ml of water, add three times the volume of absolute ethanol, and incubate at 4°C for 12 hours, then centrifuge at 4°C and 10,000 rpm for 10 minutes, collect the precipitate, and re-precipitate the supernatant once with alcohol. The two precipitates were combined, washed with absolute ethanol, and then redissolved with 100 mL of distilled water.

[0029] F...

Embodiment 2

[0032] Preparation and purification of abalone shell water-soluble matrix extract

[0033] Rinse the wrinkled abalone shells produced in Fujian, dry them naturally, and grind them into 60-120 mesh powder with a pulverizer. Weigh 3kg powder into 18L distilled water, stir and dissolve at 80°C for 3 hours, take out the mixed solution and filter it with suction, extract the residue twice with water and merge it into the mixed solution, remove the precipitated supernatant and freeze-dry it into a powder. The powder is the water-soluble matrix of abalone shell, 0.9g in total.

[0034] Dissolve 1 g of freeze-dried powder in 50 ml of water, add three times the volume of absolute ethanol, and incubate at 4°C for 12 hours, then centrifuge at 4°C and 10,000 rpm for 10 minutes, collect the precipitate, and re-precipitate the supernatant once with alcohol. The two precipitates were combined, washed with absolute ethanol, and then redissolved with 100 mL of distilled water.

[0035] For h...

Embodiment 3

[0038] Preparation and purification of abalone shell water-soluble matrix extract

[0039]Rinse the wrinkled abalone shells produced in Fujian, dry them naturally, and grind them into 60-120 mesh powder with a pulverizer. Weigh 3kg powder into 24L distilled water, stir and dissolve at 70°C for 4 hours, take out the mixed solution and filter it with suction, extract the residue twice with water and merge it into the mixed solution, remove the precipitated supernatant and lyophilize it into a powder. The powder is the water-soluble matrix of abalone shell, 0.8g in total.

[0040] Dissolve 1 g of freeze-dried powder in 50 ml of water, add three times the volume of absolute ethanol, and incubate at 4°C for 12 hours, then centrifuge at 4°C and 10,000 rpm for 10 minutes, collect the precipitate, and re-precipitate the supernatant once with alcohol. The two precipitates were combined, washed with absolute ethanol, and then redissolved with 100 mL of distilled water.

[0041] For he...

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Abstract

The invention provides a method for extracting oxidation resistant water-soluble polysaccharide of an abalone shell, which belongs to the technical field of extraction of water-soluble polysaccharide and waste utilization of abalone shells and solves the problem of low utilization ratio caused by that the abalone shell is commonly used as a medicine after being ground or directly discarded in the prior art. The extracting method comprises the following steps of: dissolving and pumping the abalone shell to remove sediments, adding an organic solvent for depositing, centrifugally separating, redissolving, removing protein by adopting a trichloroacetic acid method and removing salt and pigment by adopting a membrane separation method, to obtain the water-soluble polysaccharide. The water-soluble polysaccharide can be made into an antioxidant; and the abalone shell has rich resources, is easy for extraction and has a development potential.

Description

technical field [0001] The invention belongs to the technical field of water-soluble polysaccharide extraction and utilization of abalone shell waste, and more specifically relates to a method for extracting an antioxidant water-soluble substance from abalone shell. Background technique [0002] Judging from the current research results, oxygen free radicals are not only related to aging but also to most common human diseases, from cardiovascular and cerebrovascular diseases with the highest human mortality rate to cancer and AIDS, all are closely related to free radicals. Prevent and treat diseases by preventing the generation of free radicals and removing existing free radicals. Nowadays, synthetic antioxidants have certain toxicity and mutagenicity, and their repeated use is greatly restricted. Therefore, researchers at home and abroad are looking for safe and effective natural antioxidants. [0003] Abalone belongs to the phylum Mollusca, the class Gastropoda, the famil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 张其清郭葳梁红宝伍久林
Owner FUZHOU UNIV
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