33results about How to "Has development potential" patented technology

The invention relates to a pyrimido [5, 4-b] methotrexate compound as shown in a general formula I, an optical isomer, a preparation method, a pharmaceutical composition containing the pyrimido [5, 4-b] methotrexate compound and application. The compound has good inhibitory activity on kinase BTK at a molecular level and a cellular level, and also has good in-vivo antitumor activity and pharmacokinetic properties.

The present invention provides the structure general formula of a class of pyrazole heterocycle-containing ursolic amide derivatives, further a synthesis route and synthesis steps of the pyrazole heterocycle-containing ursolic amide derivatives, and uses of the pyrazole heterocycle-containing ursolic amide derivatives in preparation of tumor treatment drugs. According to the present invention, the in vitro ant-tumor activity test results show that the pyrazole heterocycle-containing ursolic amide derivatives can significantly kill tumor cell growth, and have the development potential in novel antitumor drugs.

The invention provides an extraction method of blue-green pigments of an abalone shell, belonging to the technical field of natural pigment extraction and abalone shell waste recycle and solving the problem that the prior art has low processing and utilizing rates of abalone shells. The extraction method comprises the following steps of: leaching the abalone shells in an acetic acid; filtrating to remove sediment; adjusting a pH value; filtering again to remove supernatant fluid; redissolving the sediment in the acetic acid; adjusting the pH value; leaching with ethyl acetate to obtain a bluepigment; and drying at 60-80 DEG C to form a solid particle pigment. The extraction method is simple, can prepare a natural blue pigment additive, and remains full shells for other purposes after pigment extraction. The abalone shells have rich resources, are easy to extract and have development potential.

The invention discloses a strain of Paenibacillus polymyxa and its application in preventing and treating various soil-borne diseases. The preservation number of Paenibacillus polymyxa ZF129 protected by the present invention is CGMCC No.17631. Paenibacillus polymyxa (Paenibacillus polymyxa) ZF129 can significantly inhibit the growth of pathogenic fungal hyphae, display broad-spectrum antagonistic activity, and have growth-promoting ability to plants. Paenibacillus polymyxa (Paenibacillus polymyxa) ZF129 exhibits excellent biocontrol properties, has potential for further development, and lays a foundation for in-depth research on the mechanism of Paenibacillus polymyxa in disease prevention.

The invention discloses an application of N-acetylated chitohexaose for inhibiting the proliferation of tumor cells. The sugar residue at the reducing end of the N-acetylated chitohexaose is N-acetylglucosamine and the sugar residue at the 2th site from the reducing end has a glucosamine structure. A natural non-toxic chitosan oligosaccharide is used as a lead compound and through selective N-acetylation and ion exchange chromatography separation, a specific structural component of N-acetylated chitohexaose having strong anti-tumor activity is screened. The N-acetylated chitohexaose has a great significance to develop efficient and low-toxic marine anti-tumor innovative drugs.

The invention relates to a method for preparing a radix angelicae pubescentis extract with a pine wood nematode killing activity and an application thereof. The method comprises the following steps: taking dry radix angelicae pubescentis powder; extracting with absolute ethyl alcohol under room temperature; performing reduced pressure distillation and concentration on the extracting solution, thereby obtaining a brown rough extract; extracting a rough ethyl alcohol extract with ethyl acetate-water extracting solution for three times, merging with ethyl acetate phase; reducing the pressure and distilling, thereby obtaining the radix angelicae pubescentis extract with higher pine wood nematode killing activity; utilizing the separating methods, such as column chromatography and recrystallization, to separate four coumarins compounds with obvious pine wood nematode killing activity, namely cnidium lactone, columbianadin, bergapten and xanthotoxin, from the radix angelicae pubescentis extract. The radix angelicae pubescentis extract in the concentration of 1mg / mL has a 95.27% correcting death rate for the pine wood nematode after 72 hours. The invention has the advantages of simple operation method, convenience in raw material resource, low cost, high efficiency, low toxicity, safety and environmental protection. The radix angelicae pubescentis has a developing potential at the aspect of controlling the pine wood nematode and can be applied to a natural preparation for killing the pine wood nematode.

A method of extracting dibutyl phthalate from common buried rubber is provided. The method includes weighing the common buried rubber, performing ultrafine smashing, adding ethyl acetate, extracting, centrifuging, precipitating, adding ethanol having a concentration of 95%, extracting, centrifuging to obtain a supernatant liquid, concentrating the supernatant liquid, drying, adding an ethanol water solution having a concentration of 80%, dissolving under the function of ultrasonic waves until the concentration is 15-50 mg / mL, and separating through a C18 reverse phase silica gel chromatographic column, wherein a binary mobile phase adopting purified water as an A phase and methanol as a B phase is adopted; elution is performed in a manner that the mobile phase in which the concentration of the methanol is 20% is adopted for 5 min, then the mobile phase in which the concentration of the methanol is 40% is adopted for 7 min, then the mobile phase in which the concentration of the methanol is 80% is adopted for 13 min and finally the mobile phase in which the concentration of the methanol is 100% is adopted for 5 min; and eluate from the 21 min to the 22 min is collected according to an ultraviolet absorption spectrum, concentrated and freeze-dried in vacuum to obtain the dibutyl phthalate the purity of which is 99% or above. The method is stable in separation, high in repeatability, high in preparation amount and simple and time-saving in operation.