33results about How to "Has development potential" patented technology

The invention provides a method for extracting oxidation resistant water-soluble polysaccharide of an abalone shell, which belongs to the technical field of extraction of water-soluble polysaccharide and waste utilization of abalone shells and solves the problem of low utilization ratio caused by that the abalone shell is commonly used as a medicine after being ground or directly discarded in the prior art. The extracting method comprises the following steps of: dissolving and pumping the abalone shell to remove sediments, adding an organic solvent for depositing, centrifugally separating, redissolving, removing protein by adopting a trichloroacetic acid method and removing salt and pigment by adopting a membrane separation method, to obtain the water-soluble polysaccharide. The water-soluble polysaccharide can be made into an antioxidant; and the abalone shell has rich resources, is easy for extraction and has a development potential.

The invention discloses a ginseng fungus health-care beverage. according to the beverage, per 1000 L of purified water is added with the following compositions: 0.1-12 Kg of ginseng, 0.1-7 Kg of hericium erinaceus, 2-15 Kg of aloe vera juice, 0.3 Kg of auricularia auricula, 0-110 Kg of white sugar, 0.0003-0.003 Kg of vitamin B6, 0.0001 Kg of vitamin C, 0.1-5 Kg of royal jelly, 2-10 Kg of matrimony vine, and a little amount of xylitol, carboxymethylcellulose sodium, citric acid, sodium citrate, potassium sorbate, a lemon yellow solution with the mass fraction of 1%, a sunset yellow solution with the mass fraction of 1%, malic acid and ginseng essence. The ginseng fungus health-care beverage is prepared by employing natural products as raw materials and performing high-temperature sterilization, loading, sealing and secondary sterilization, is capable of improving human body immunocompetence, resisting tumors, reducing blood sugar, reducing cholesterol, delaying ageing, resisting fatigue and the like, and gives play to health-care effect.

The invention provides a method of extracting ergosterol peroxide from armillaria luteo-virens. The method comprises the steps that armillaria luteo-virens sporocarps are used as a raw material, ground into powder and then subjected to ethyl acetate extraction; supernatant is taken for one-dimensional liquid phase separation, a Silica normal-phase silica gel chromatographic column and binary organic phases of A-phase normal hexane and B-phase ethyl alcohol are adopted, and the B-phase concentration is subjected to gradient elution from 0% to 75%; a one-dimensional component is obtained for two-dimensional liquid phase separation, and the elution mode is changed to the mode that the B-phase concentration is subjected to isocratic elution to 4%-8%; a two-dimensional component is obtained for three-dimensional liquid phase separation, the elution mode is changed to the mode that the B-phase concentration is subjected to isocratic elution to 2%-3%, and the target product is obtained. According to the method, efficient liquid phase chromatography is adopted for separation and purification, the separation method is stable, repeatability is high, the prepared amount is large, operation is simple, and time is saved. Experiments find that the compound has an inhibitory effect on Hep-g2 and A549, thereby having the potential of being developed into an anticancer drug and providing a theoretical basis for new drug research and development.

The invention discloses a paenibacillus polymyxa and application thereof in prevention and treatment of various soil-borne diseases. The preservation number of the paenibacillus polymyxa ZF129 protected by the invention is CGMCC (China General Microbiological Culture Collection Center) No.17631. The paenibacillus polymyxa ZF129 can remarkably inhibit the growth of pathogenic fungus hyphae, shows broad-spectrum antagonistic activity, and has the capability of promoting the growth of plants. The paenibacillus polymyxa ZF129 shows an excellent biocontrol character, has a further development potential, and lays a certain foundation for further study of the paenibacillus on the mechanism of disease prevention.

The invention discloses dried noodles containing broussonetia papyrifera leaves. The noodles comprise the following raw materials in parts by weight; flour 90-110, broussonetia papyrifera leaves 15-20, salt 0.5-4, edible alkali 0.1-0.3, sodium alginate 0.1-0.3, and water 25-30. The invention also discloses a preparation method of the dried noodles containing broussonetia papyrifera leaves. The dried noodles have smooth surface and are uniform, the dried noodles are light green in color, taste delicate, not sticky and gritty, is firm, smooth and tasty, and has the special scent of broussonetia papyrifera leaves, does not has moldy smell, sour odor and other peculiar smell; after the noodles are cooked, the noodles are not easy to get burnt, the natural broken noodle rate and the cooked broken noodle rate are zero, and the cooking loss rate is relatively low. The noodles produced by the above formula meet the demand of popular tastes. The dried noodles containing broussonetia papyrifera leaves have wide market development prospect, and are a natural green health care food.

The invention provides a process for producing maltose vinegar stachys sieboldii can, which comprises the following steps: fermenting maltose by adding cold boiled water to prepare maltose mother vinegar; fermenting the maltose mother vinegar by adding maltose and mountain spring water to prepare maltose vinegar; adding proper amount of edible salt and sugar into the maltose vinegar, or also adding pepper water to prepare maltose vinegar soup; and preparing the stachys sieboldii can from the soup. The prepared maltose vinegar stachys sieboldii can has pure taste, unique flavor and great development potential.

The invention discloses an inclination type aperture measuring device and a method thereof. The device comprises a center rod, a plurality of couples of radial extending beams, extending beam adjustable sections, positioning bolts, aperture self-adapting rods, pulleys and torsion springs; the radial extending beams are fixed to the center rod symmetrically, each couple of the radial extending beams are distributed at two sides of the center rod and located in the same line, and a side-incline sensor is carried in the center rod; the corresponding extending beam adjustable section is arranged at the end, far from the center rod, of each radial extending beam, the aperture self-adapting rods are hinged to the extending beam adjustable sections, and the torsion springs used for exerting external expansion force to the aperture self-adapting rods are arranged at the hinging parts; and the pulleys are arranged at the tail ends of the aperture self-adapting rods which carry the side-incline sensors. The inclination type aperture measuring device is simple in structure, can be subjected to waterproof sealing easily, can achieve durable use of equipment and has good equipment reliability. According to the device, operation is easy, the size of the device is small, the radial extending beams are adjustable, and the device has considerable project adaptability and promotional value.

The invention provides a method for extracting oxidation resistant water-soluble polysaccharide of an abalone shell, which belongs to the technical field of extraction of water-soluble polysaccharide and waste utilization of abalone shells and solves the problem of low utilization ratio caused by that the abalone shell is commonly used as a medicine after being ground or directly discarded in theprior art. The extracting method comprises the following steps of: dissolving and pumping the abalone shell to remove sediments, adding an organic solvent for depositing, centrifugally separating, redissolving, removing protein by adopting a trichloroacetic acid method and removing salt and pigment by adopting a membrane separation method, to obtain the water-soluble polysaccharide. The water-soluble polysaccharide can be made into an antioxidant; and the abalone shell has rich resources, is easy for extraction and has a development potential.

The invention relates to a method for preparing a radix angelicae pubescentis extract with a pine wood nematode killing activity and an application thereof. The method comprises the following steps: taking dry radix angelicae pubescentis powder; extracting with absolute ethyl alcohol under room temperature; performing reduced pressure distillation and concentration on the extracting solution, thereby obtaining a brown rough extract; extracting a rough ethyl alcohol extract with ethyl acetate-water extracting solution for three times, merging with ethyl acetate phase; reducing the pressure and distilling, thereby obtaining the radix angelicae pubescentis extract with higher pine wood nematode killing activity; utilizing the separating methods, such as column chromatography and recrystallization, to separate four coumarins compounds with obvious pine wood nematode killing activity, namely cnidium lactone, columbianadin, bergapten and xanthotoxin, from the radix angelicae pubescentis extract. The radix angelicae pubescentis extract in the concentration of 1mg / mL has a 95.27% correcting death rate for the pine wood nematode after 72 hours. The invention has the advantages of simple operation method, convenience in raw material resource, low cost, high efficiency, low toxicity, safety and environmental protection. The radix angelicae pubescentis has a developing potential at the aspect of controlling the pine wood nematode and can be applied to a natural preparation for killing the pine wood nematode.

The present invention provides the structure general formula of a class of pyrazole heterocycle-containing ursolic amide derivatives, further a synthesis route and synthesis steps of the pyrazole heterocycle-containing ursolic amide derivatives, and uses of the pyrazole heterocycle-containing ursolic amide derivatives in preparation of tumor treatment drugs. According to the present invention, the in vitro ant-tumor activity test results show that the pyrazole heterocycle-containing ursolic amide derivatives can significantly kill tumor cell growth, and have the development potential in novel antitumor drugs.

The invention discloses height-adjustable horizontal force output equipment which comprises a moving seat, a U-shaped plate is mounted on the moving seat, a rectangular frame is slidably connected into the U-shaped plate, a servo motor is mounted at the bottom of the moving seat, a driving gear is mounted at the output end of the servo motor, two threaded cylinders are rotatably connected to the upper end of the moving seat, and a driving gear is mounted at the output end of the driving gear. A transmission mechanism is arranged between the two threaded cylinders, screw rods are in threaded connection with the interiors of the two threaded cylinders, the upper ends of the two screw rods are fixedly connected with the bottom of the rectangular frame, and a driven gear is fixedly installed outside one of the threaded cylinders. Through cooperation of the limiting wheel and the warning lamp, a load-bearing cable can be subjected to horizontal detection, through cooperation of the servo motor, the threaded cylinder and the screw rod, the load-bearing cable can be subjected to horizontal adjustment, so that the longitudinal force of the balance weight can be changed into horizontal force, and the tray and the balance weight can be shielded and prevented from falling off and being damaged; and an experimenter can be protected.

The invention discloses a lifting wing for a submersible, comprising a rotating wing, a fixed wing, a rotating shaft, a shaft sleeve, a micro speed changer, a mounting bottom plate, a stepping motor and a stepping motor driver, wherein the fixed wing and the shaft sleeve are fixedly connected with a submersible shell; the shaft sleeve penetrates through the submersible shell; the rotating shaft is arranged in the shaft sleeve through a bearing; the outer end of the rotating shaft is connected with the rotating wing, and the inner end of the rotating shaft is connected with the stepping motor through the micro speed changer; and the micro speed changer and the stepping motor are fixed on the mounting bottom plate. The device can provide ascending force or descending force according to a positive or negative angle of attack of the lifting wing, so that the submersible can flexibly ascend or descend in water.

The invention relates to a pyrimido [5, 4-b] methotrexate compound as shown in a general formula I, an optical isomer, a preparation method, a pharmaceutical composition containing the pyrimido [5, 4-b] methotrexate compound and application. The compound has good inhibitory activity on kinase BTK at a molecular level and a cellular level, and also has good in-vivo antitumor activity and pharmacokinetic properties.

The invention provides a method for extracting an antineoplastic active component from Armillaria luteo-virens. The method comprises the following steps: grinding Armillaria luteo-virens fruiting body into a powder and extracting with ethyl acetate; fetching a supernatant and carrying out one-dimension liquid phase separation: adopting a Silica normal-phase silica gel column and binary organic phases including A phase n-hexane and B phase ethanol, and carrying out gradient elution with concentration of the B phase being 0-75%; collecting 10-14 min eluant and carrying out two-dimension liquid phase separation by the elution mode of isocratic elution with concentration of the B phase being 4-8%; and collecting 6-8 min eluant and carrying out three-dimensional liquid phase separation by the elution mode of isocratic elution with concentration of the B phase being 2-3%, and collecting 7.5-12 min eluant and drying by evaporation so as to obtain a target product. According to the method, separation and purification are carried out by high performance liquid chromatography. Repeatability is high, preparation quantity is large, and operation is simple and time-saving. It is found through experiments that the compound has an inhibitory effect on Hep-g2. Thus, the compound has an anti-cancer drug developing potential and provides theoretical foundation for research and development of new drugs.

The invention relates to a method for extracting alcohol-soluble antioxidant substances from the visceral organs of an abalone, belonging to the technical field of extraction of antioxidant substances in the visceral organs of the abalone. The method for extracting comprises the steps of: pulping the visceral organs of the abalone, soaking and extracting, removing heavy metals, concentrating, conducting alcohol precipitation, rectifying, concentrating and drying to obtain the alcohol-soluble antioxidant substances from the visceral organs of the abalone. The alcohol-soluble antioxidant substances in the visceral organs of the abalone related by the invention contain rich protein, amino acid, saccharides, lipid and the like, have good antioxidant activity, and can be used for preparing amino acid supplemented nourishments and various antioxidant preparations.

The present invention provides the structure general formula of a class of pyrazole heterocycle-containing ursolic amide derivatives, further a synthesis route and synthesis steps of the pyrazole heterocycle-containing ursolic amide derivatives, and uses of the pyrazole heterocycle-containing ursolic amide derivatives in preparation of tumor treatment drugs. According to the present invention, the in vitro ant-tumor activity test results show that the pyrazole heterocycle-containing ursolic amide derivatives can significantly kill tumor cell growth, and have the development potential in novel antitumor drugs.

The invention provides an extraction method of blue-green pigments of an abalone shell, belonging to the technical field of natural pigment extraction and abalone shell waste recycle and solving the problem that the prior art has low processing and utilizing rates of abalone shells. The extraction method comprises the following steps of: leaching the abalone shells in an acetic acid; filtrating to remove sediment; adjusting a pH value; filtering again to remove supernatant fluid; redissolving the sediment in the acetic acid; adjusting the pH value; leaching with ethyl acetate to obtain a bluepigment; and drying at 60-80 DEG C to form a solid particle pigment. The extraction method is simple, can prepare a natural blue pigment additive, and remains full shells for other purposes after pigment extraction. The abalone shells have rich resources, are easy to extract and have development potential.

The invention provides an extraction method of an anticancer active monomer in Curcuma zedoaria. The method includes: taking Curcuma zedoaria tuber as the raw material, grinding the Curcuma zedoaria tuber into powder, then conducting ethanol extraction, concentrating the filtrate, then dissolving the product in a n-hexane-ethanol mixed solvent; conducting one-dimensional liquid chromatography separation: employing a normal phase silica gel chromatographic column, adopting a binary mobile phase with n-hexane as the phase A and ethanol as the phase B, carrying out elution with the phase B at a concentration of 0-50%, and collecting the 15.5-17min eluent; performing dissolving with the n-hexane-ethanol mixed solvent; conducting two-dimensional liquid chromatography separation: employing an Acchrom X-Amide chromatographic column, adopting a binary mobile phase with n-hexane as the phase A and ethanol as the phase B, carrying out elution with the phase B at a concentration of 0-30%, and collecting the 17-18min eluent so as to obtain a target product. The invention provides a new compound never reported before, the process has the characteristics of simplicity, low cost, large preparation capacity and good repeatability, and the obtained monomer compound has high purity, good biological activity and anticancer drug development potential.

The invention discloses an application of N-acetylated chitohexaose for inhibiting the proliferation of tumor cells. The sugar residue at the reducing end of the N-acetylated chitohexaose is N-acetylglucosamine and the sugar residue at the 2th site from the reducing end has a glucosamine structure. A natural non-toxic chitosan oligosaccharide is used as a lead compound and through selective N-acetylation and ion exchange chromatography separation, a specific structural component of N-acetylated chitohexaose having strong anti-tumor activity is screened. The N-acetylated chitohexaose has a great significance to develop efficient and low-toxic marine anti-tumor innovative drugs.

The invention provides a new compound, which has a molecular formula of C15H20O3, a molecular weight of 248, and a Chinese name of 8-hydroxy-Germa-1, 7-diene-5one-8, 12-lactone. The preparation method includes: taking curcuma zedoaria tuber as the raw material, conducting grinding into powder, then performing ethanol extraction, concentrating the filtrate, then dissolving the product in a n-hexane-ethanol mixed solvent; conducting one-dimensional liquid chromatography separation: employing a normal phase silica gel chromatographic column, adopting a binary mobile phase with n-hexane as the phase A and ethanol as the phase B, carrying out elution with the phase B at a concentration of 0-50%, and collecting the 15.5-17min eluent; performing dissolving with the n-hexane-ethanol mixed solvent; conducting two-dimensional liquid chromatography separation: employing an Acchrom X-Amide chromatographic column, adopting a binary mobile phase with n-hexane as the phase A and ethanol as the phase B, carrying out elution with the phase B at a concentration of 0-30%, and collecting the 17-18min eluent so as to obtain a target product. The new compound is proved to have anti-liver cancer and anti-lung cancer biological activity and can be used for preparation of related drugs.

The invention provides an extraction method of an anticancer active monomer in Curcuma zedoaria. The method includes: taking Curcuma zedoaria tuber as the raw material, grinding the Curcuma zedoaria tuber into powder, then conducting ethanol extraction, concentrating the filtrate, then dissolving the product in a n-hexane-ethanol mixed solvent; conducting one-dimensional liquid chromatography separation: employing a normal phase silica gel chromatographic column, adopting a binary mobile phase with n-hexane as the phase A and ethanol as the phase B, carrying out elution with the phase B at a concentration of 0-50%, and collecting the 15.5-17min eluent; performing dissolving with the n-hexane-ethanol mixed solvent; conducting two-dimensional liquid chromatography separation: employing an Acchrom X-Amide chromatographic column, adopting a binary mobile phase with n-hexane as the phase A and ethanol as the phase B, carrying out elution with the phase B at a concentration of 0-30%, and collecting the 17-18min eluent so as to obtain a target product. The invention provides a new compound never reported before, the process has the characteristics of simplicity, low cost, large preparation capacity and good repeatability, and the obtained monomer compound has high purity, good biological activity and anticancer drug development potential.

The invention provides a new compound, which has a molecular formula of C15H20O3, a molecular weight of 248, and a Chinese name of 8-hydroxy-Germa-1, 7-diene-5one-8, 12-lactone. The preparation method includes: taking curcuma zedoaria tuber as the raw material, conducting grinding into powder, then performing ethanol extraction, concentrating the filtrate, then dissolving the product in a n-hexane-ethanol mixed solvent; conducting one-dimensional liquid chromatography separation: employing a normal phase silica gel chromatographic column, adopting a binary mobile phase with n-hexane as the phase A and ethanol as the phase B, carrying out elution with the phase B at a concentration of 0-50%, and collecting the 15.5-17min eluent; performing dissolving with the n-hexane-ethanol mixed solvent; conducting two-dimensional liquid chromatography separation: employing an Acchrom X-Amide chromatographic column, adopting a binary mobile phase with n-hexane as the phase A and ethanol as the phase B, carrying out elution with the phase B at a concentration of 0-30%, and collecting the 17-18min eluent so as to obtain a target product. The new compound is proved to have anti-liver cancer and anti-lung cancer biological activity and can be used for preparation of related drugs.

The invention provides a general structural formula of an ursolic acid derivative containing a parazole heterocycle. The invention further provides a synthetic route of the ursolic acid derivative containing the parazole heterocycle and synthesis steps of the ursolic acid containing the parazole heterocycle. The invention also provides application of the ursolic acid derivative containing the parazole heterocycle in preparation of a drug used for treating tumor. The ursolic acid derivative as well as synthesis and application thereof, which are provided by the invention, have the advantages that in vitro anti-tumor activity tests show that the compound has obvious inhibiting effect on tumor growth, provides a new target spot and a new treatment strategy for development of anti-tumor drugs and has potential of being developed into a novel anti-tumor drug.

The invention relates to the technical field of medicine, in particular to an N-aryl anthranilamide acid compound anti-intracerebral tumor active compound, preparation and application thereof, wherein the structural general formula is shown in the specification, R1 is one or more of halogen F or Cl or Br or I, alkyl, heteroalkane, amino and nitryl, the substitution positions of R1 on a benzene ring are 2-site, 3-site and 4-site, the number of substituents is monosubstitution or polysubstitution, R2 is one or more of halogen F or Cl or Br or I, alkyl, heteroalkane, amino and nitryl, the substitution positions of R2 on the benzene ring are 2-site, 3-site and 4-site, the number of substituents is monosubstitution or polysubstitution, R3 is a heterocyclic ring or a benzoheterocyclic ring, n is 1-3, and X is N or CH. The compound disclosed by the invention has a remarkable COX-2 inhibition effect and a topoisomerase inhibition effect, has the effects of remarkably inhibiting proliferation, invasion and migration of various tumors, especially brain tumors such as glioma and the like in vivo and in vitro, retarding cycle and the like, and is a novel multi-target antitumor drug.

The invention provides a method for extracting dibutyl phthalate from triangular prisms, which comprises the following steps: take triangular prisms and add ethyl acetate to extract after superfine grinding, precipitate after centrifugation and add 95% ethanol for extraction, and centrifuge to extract dibutyl phthalate. Concentrate and dry the supernatant; add 80% methanol-water solution to sonicate to 15-50mg / ml, and 18 Reversed-phase silica gel chromatography column for liquid chromatography separation, using A pure water, B methanol binary mobile phase, the elution method is 20% methanol-water elution 5min, 40% methanol-water elution 7min, 80% methanol-water elution Water was eluted for 13 minutes, 100% methanol was eluted for 5 minutes, and the eluate was collected for 21-22 minutes according to the ultraviolet absorption spectrum, concentrated and vacuum freeze-dried to obtain dibutyl phthalate with a purity of more than 99%. The method has the advantages of stable separation method, high repeatability, large preparation amount, simple and time-saving operation.

The invention discloses a strain of Paenibacillus polymyxa and its application in preventing and treating various soil-borne diseases. The preservation number of Paenibacillus polymyxa ZF129 protected by the present invention is CGMCC No.17631. Paenibacillus polymyxa (Paenibacillus polymyxa) ZF129 can significantly inhibit the growth of pathogenic fungal hyphae, display broad-spectrum antagonistic activity, and have growth-promoting ability to plants. Paenibacillus polymyxa (Paenibacillus polymyxa) ZF129 exhibits excellent biocontrol properties, has potential for further development, and lays a foundation for in-depth research on the mechanism of Paenibacillus polymyxa in disease prevention.

The invention discloses a method for preparing D-mannitol through microbial metabolism of penicillium. The penicillium microbe is a mutant strain obtained through artificial mutation on endogenous penicillium of the stem of wild gastrodia elata from Zhaotong, Yunnan, and abundant D-mannitol is obtained through metabolism after fermentation. The method comprises the following main steps: fermentingthe strain under the suitable culture condition, carrying out solvent ultrasonic extraction and carrying out concentration, thus abundant D-mannitol is prepared by combining with column chromatography, wherein the concentration in the fermented liquid can achieve 1.14-1.26 g / L, and the purity can achieve 95-98% after extraction and separation. The method is low in cost, the process is simple, theenvironment protection is realized, the mass production is available, the demand of low carbon economy is satisfied, and a solid foundation is laid for the later production of D-mannitol and the development of relevant products.

The invention discloses an application of N-acetylated chitohexaose for inhibiting the proliferation of tumor cells. The sugar residue at the reducing end of the N-acetylated chitohexaose is N-acetylglucosamine and the sugar residue at the 2th site from the reducing end has a glucosamine structure. A natural non-toxic chitosan oligosaccharide is used as a lead compound and through selective N-acetylation and ion exchange chromatography separation, a specific structural component of N-acetylated chitohexaose having strong anti-tumor activity is screened. The N-acetylated chitohexaose has a great significance to develop efficient and low-toxic marine anti-tumor innovative drugs.

The invention relates to a method for preparing a radix angelicae pubescentis extract with a pine wood nematode killing activity and an application thereof. The method comprises the following steps: taking dry radix angelicae pubescentis powder; extracting with absolute ethyl alcohol under room temperature; performing reduced pressure distillation and concentration on the extracting solution, thereby obtaining a brown rough extract; extracting a rough ethyl alcohol extract with ethyl acetate-water extracting solution for three times, merging with ethyl acetate phase; reducing the pressure and distilling, thereby obtaining the radix angelicae pubescentis extract with higher pine wood nematode killing activity; utilizing the separating methods, such as column chromatography and recrystallization, to separate four coumarins compounds with obvious pine wood nematode killing activity, namely cnidium lactone, columbianadin, bergapten and xanthotoxin, from the radix angelicae pubescentis extract. The radix angelicae pubescentis extract in the concentration of 1mg / mL has a 95.27% correcting death rate for the pine wood nematode after 72 hours. The invention has the advantages of simple operation method, convenience in raw material resource, low cost, high efficiency, low toxicity, safety and environmental protection. The radix angelicae pubescentis has a developing potential at the aspect of controlling the pine wood nematode and can be applied to a natural preparation for killing the pine wood nematode.

A method of extracting dibutyl phthalate from common buried rubber is provided. The method includes weighing the common buried rubber, performing ultrafine smashing, adding ethyl acetate, extracting, centrifuging, precipitating, adding ethanol having a concentration of 95%, extracting, centrifuging to obtain a supernatant liquid, concentrating the supernatant liquid, drying, adding an ethanol water solution having a concentration of 80%, dissolving under the function of ultrasonic waves until the concentration is 15-50 mg / mL, and separating through a C18 reverse phase silica gel chromatographic column, wherein a binary mobile phase adopting purified water as an A phase and methanol as a B phase is adopted; elution is performed in a manner that the mobile phase in which the concentration of the methanol is 20% is adopted for 5 min, then the mobile phase in which the concentration of the methanol is 40% is adopted for 7 min, then the mobile phase in which the concentration of the methanol is 80% is adopted for 13 min and finally the mobile phase in which the concentration of the methanol is 100% is adopted for 5 min; and eluate from the 21 min to the 22 min is collected according to an ultraviolet absorption spectrum, concentrated and freeze-dried in vacuum to obtain the dibutyl phthalate the purity of which is 99% or above. The method is stable in separation, high in repeatability, high in preparation amount and simple and time-saving in operation.