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The method and its application of extracting dibutyl phthalate from triangular

A technology of dibutyl phthalate and ethyl acetate, applied in the application of anti-lung cancer drugs, and the field of preparation of anti-lung cancer, can solve the problems of little research on small molecule pharmacodynamics, little research on small molecular compounds, etc., and achieve practical High performance, stable properties, and low reagent consumption

Active Publication Date: 2018-12-14
TIANJIN YAOYU BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Because this substance is defined as a harmful chemical product by the public, and is limited to obtain through synthetic methods, there is little research on the small molecule compound that is naturally occurring in the molecule itself, and it is limited to the above-mentioned application, so many scholars study Its toxicological effects, such as reproductive toxicity research, neurotoxicity research, acute toxicity research, etc., but there are relatively few studies on the efficacy of this small molecule

Method used

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  • The method and its application of extracting dibutyl phthalate from triangular
  • The method and its application of extracting dibutyl phthalate from triangular
  • The method and its application of extracting dibutyl phthalate from triangular

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The present embodiment extracts the method for dibutyl phthalate monomer compound from triangular, comprises the following steps:

[0054] (1) Take the dried triangular ribs as the raw material, put them into a superfine pulverizer for superfine pulverization, and use 4°C water as the internal cooling liquid of the superfine pulverizer during the pulverization process, and the superfine pulverization time is 12 minutes to obtain superfine powder.

[0055] (2) Take 3Kg of the above-mentioned triangular superfine powder and extract it three times with ethyl acetate. For the first extraction: add 3kg of superfine powder to 24L ethyl acetate, extract for 70min, and after the extraction is completed, centrifuge at 25°C and 6000rpm for 30min to collect the residue. The second extraction: add the residue of the first extraction into 12L ethyl acetate, extract for 70min, and after the extraction is completed, centrifuge at 25°C and 6000rpm for 30min to collect the residue. The ...

Embodiment 2

[0062] Embodiment 2: the test of inhibiting the growth of human lung cancer A549 cells

[0063] Cell culture:

[0064] at 5% C0 2 , 37° C., under saturated humidity, cultured human lung cancer A549 cells with F-12K (10% FBS+1% PS) medium, and selected the cells growing in the logarithmic phase as experimental cells. After counting the cells, dilute it with medium to obtain a certain concentration of cell suspension.

[0065] Cell growth monitoring:

[0066] Put the cell real-time monitor in 5% C0 2 , 37°C saturated humidity incubator. Take an 8-well plate, add 150 μL of F-12K medium to each well, and put it into the real-time cell monitor for baseline. After the baseline, take out the eight-well plate and add 345 μL of diluted A549 cell suspension to each well to reach the number of cells in each well. About 2×10 4 Let stand for 3 minutes, and observe whether the cells are uniform under an inverted microscope. Add 5 μL of the diluted drug to each well to a final concent...

Embodiment 3

[0071] Embodiment 3: the test of suppressing the growth of human liver cancer Hep-G2 cells

[0072] Cell culture:

[0073] at 5% C0 2 , 37° C., under saturated humidity, cultured human lung cancer Hep-G2 cells with MEM (10% FBS, 1% PS) medium, and selected cells with good growth status as experimental cells. After counting the cells, dilute it with medium to obtain a certain concentration of cell suspension.

[0074] Cell growth monitoring:

[0075] Put the cell real-time monitor in 5% C0 2 , 37°C saturated humidity incubator. Take an 8-well plate, add 150 μL of MEM medium to each well, put it into a real-time cell monitor for baseline, take out the eight-well plate after the baseline, and add 345 μL of diluted Hep-G2 cell suspension to each well to reach the number of cells in each well. About 4×10 4 Let stand for 3 minutes, and observe whether the cells are uniform under an inverted microscope. Add 5 μL of the diluted drug to each well until the final concentration of...

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Abstract

A method of extracting dibutyl phthalate from common buried rubber is provided. The method includes weighing the common buried rubber, performing ultrafine smashing, adding ethyl acetate, extracting, centrifuging, precipitating, adding ethanol having a concentration of 95%, extracting, centrifuging to obtain a supernatant liquid, concentrating the supernatant liquid, drying, adding an ethanol water solution having a concentration of 80%, dissolving under the function of ultrasonic waves until the concentration is 15-50 mg / mL, and separating through a C18 reverse phase silica gel chromatographic column, wherein a binary mobile phase adopting purified water as an A phase and methanol as a B phase is adopted; elution is performed in a manner that the mobile phase in which the concentration of the methanol is 20% is adopted for 5 min, then the mobile phase in which the concentration of the methanol is 40% is adopted for 7 min, then the mobile phase in which the concentration of the methanol is 80% is adopted for 13 min and finally the mobile phase in which the concentration of the methanol is 100% is adopted for 5 min; and eluate from the 21 min to the 22 min is collected according to an ultraviolet absorption spectrum, concentrated and freeze-dried in vacuum to obtain the dibutyl phthalate the purity of which is 99% or above. The method is stable in separation, high in repeatability, high in preparation amount and simple and time-saving in operation.

Description

technical field [0001] The present invention relates to the technical field of traditional Chinese medicine extraction, in particular to a method for extracting dibutyl phthalate monomer compound from trigonum, and the application of the dibutyl phthalate in the preparation of anti-liver cancer and anti-lung cancer drugs . Background technique [0002] Dibutyl phthalate is a small molecular compound usually obtained by organic synthesis. It is a colorless, transparent or slightly yellow oily liquid, and its molecular formula is C 16 h 22 o 4 , the molecular weight is 278.35, and its skeleton is composed of a structure similar to a four-membered ring. The structural formula is as follows: [0003] [0004] Today's applications of dibutyl phthalate mainly include the following aspects: [0005] 1. Used as gas chromatography stationary liquid and solvent; [0006] 2. Used as a plasticizer, mainly used as a plasticizer for nitrocellulose, cellulose acetate, polyvinyl chl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C69/80C07C67/48C07C67/56A61K31/235A61P35/00
Inventor 张耀洲庞莉顾朋嫒杜思邈李聪聪姜睿
Owner TIANJIN YAOYU BIOLOGICAL TECH
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