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Penicillium, method for preparing D-mannitol through microbial metabolism of penicillium and applications

A technology of Penicillium and mannitol, which is applied in the field of preparation of D-mannitol, can solve problems such as the development restriction of industrial production of D-mannitol, and achieve the effects of no environmental pollution, mild reaction conditions and simple equipment requirements.

Inactive Publication Date: 2018-09-28
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Until the technology of the synthetic method matures, these two methods are limited by raw material resources, extraction yield, climatic conditions, energy consumption, etc., and the development of industrial production of D-mannitol is restricted.

Method used

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  • Penicillium, method for preparing D-mannitol through microbial metabolism of penicillium and applications
  • Penicillium, method for preparing D-mannitol through microbial metabolism of penicillium and applications
  • Penicillium, method for preparing D-mannitol through microbial metabolism of penicillium and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Cell activation: inoculate Penicillium T2-8-10 into the PDA slant medium sterilized at 121°C for 30 minutes, culture it in a constant temperature incubator at 28°C for 5 days, and then place it in a refrigerator at 4°C for use.

[0028] Strain preparation: Inoculate activated bacteria into PDB medium sterilized at 121°C for 30 minutes, and ferment for 3 days at 28°C and 150 rpm.

[0029] Fermentation process: Add the prepared strains according to the volume fraction of 10% into the fermentation medium sterilized at 121° C. for 30 minutes (the components of the fermentation medium in terms of volume mass percentage: 2.8% glucose, 0.2% dipotassium hydrogen phosphate, 0.2% magnesium sulfate heptahydrate, 20% potato juice 1L, 2 mg per liter of thiamine), at 25° C., 150 rpm for 9 days of fermentation.

[0030] After the fermentation, the fermentation liquid and the cells were ultrasonically broken for 30 minutes, and analyzed by high-performance liquid chromatography, the co...

Embodiment 2

[0033] Cell activation: inoculate Penicillium T2-8-10 into the PDA slant medium sterilized at 121°C for 30 minutes, culture it in a constant temperature incubator at 28°C for 5 days, and then place it in a refrigerator at 4°C for use.

[0034] Strain preparation: Inoculate activated bacteria into PDB medium sterilized at 121°C for 30 minutes, and ferment for 3 days at 28°C and 150 rpm.

[0035] Fermentation process: Add the prepared strains according to the volume fraction of 5% into the fermentation medium sterilized at 121° C. for 30 minutes (the components of the fermentation medium in terms of volume mass percentage: 2% glucose, 0.1% dipotassium hydrogen phosphate, 0.1% magnesium sulfate heptahydrate, 20% potato juice 1L, 2 mg per liter of thiamine), 28° C., 150 rpm for 9 days of fermentation.

[0036] After the fermentation, the fermentation liquid and the cells were ultrasonically broken for 30 minutes, and analyzed by high-performance liquid chromatography, the concentr...

Embodiment 3

[0039] Cell activation: Inoculate Penicillium T2-8-10 into the PDA slant medium sterilized at 121°C for 30 minutes, culture it in a constant temperature incubator at 28°C for 5 days, and then place it in a refrigerator at 4°C. , Fermentation 3d under 150rpm.

[0040] Fermentation process: Add the prepared strains according to the volume fraction of 10% into the fermentation medium sterilized at 121° C. for 30 minutes (the components of the fermentation medium in terms of volume mass percentage: 0.4% glucose, 0.1% dipotassium hydrogen phosphate, 0.1% magnesium sulfate heptahydrate, 20% potato juice 1L, 2 mg per liter of thiamine), at 28° C., 150 rpm for 9 days of fermentation.

[0041] After the fermentation, the fermentation liquid and the cells were ultrasonically broken for 15 minutes, and analyzed by high performance liquid chromatography, the concentration of D-mannitol in the fermentation liquid was 1.26 g / L.

[0042] The fermented liquid was extracted twice with equal vol...

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Abstract

The invention discloses a method for preparing D-mannitol through microbial metabolism of penicillium. The penicillium microbe is a mutant strain obtained through artificial mutation on endogenous penicillium of the stem of wild gastrodia elata from Zhaotong, Yunnan, and abundant D-mannitol is obtained through metabolism after fermentation. The method comprises the following main steps: fermentingthe strain under the suitable culture condition, carrying out solvent ultrasonic extraction and carrying out concentration, thus abundant D-mannitol is prepared by combining with column chromatography, wherein the concentration in the fermented liquid can achieve 1.14-1.26 g / L, and the purity can achieve 95-98% after extraction and separation. The method is low in cost, the process is simple, theenvironment protection is realized, the mass production is available, the demand of low carbon economy is satisfied, and a solid foundation is laid for the later production of D-mannitol and the development of relevant products.

Description

Technical field: [0001] The invention relates to the preparation of D-mannitol, in particular to a strain of Penicillium T2-8-10 and a method and application for preparing D-mannitol through its microbial metabolism. Background technique: [0002] In recent years, because microorganisms have the characteristics of short metabolic cycle, mild conversion reaction conditions, few by-products, and strong stereoselectivity, the method of preparing specific compounds by fermentation of microorganisms (fungi, bacteria, and algae) has attracted more and more attention. In actual production, there have been a large number of application examples to produce some compounds with research value or economic value through microbial fermentation. [0003] D-mannitol, the Chinese name is hexyl alcohol, white needle-like crystals. D-mannitol is non-hygroscopic and has good chemical stability. It can be used as a diuretic in medicine to reduce intracranial pressure and intraocular pressure. I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P7/18C12R1/80
CPCC12P7/18C12N1/145C12R2001/80
Inventor 丁中涛周皓杨亚滨段荣婷
Owner YUNNAN UNIV
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