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Method for extracting alcohol-soluble antioxidant substances from visceral organs of abalone

A technology of antioxidant substances and extraction methods, applied in the fields of antidote, food science, drug combination, etc., to achieve the effect of improving product quality, good rehydration, and no loss of nutrients

Active Publication Date: 2013-06-05
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no report on the extraction of alcohol-soluble antioxidant substances from abalone and the analysis of its antioxidant activity

Method used

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  • Method for extracting alcohol-soluble antioxidant substances from visceral organs of abalone
  • Method for extracting alcohol-soluble antioxidant substances from visceral organs of abalone
  • Method for extracting alcohol-soluble antioxidant substances from visceral organs of abalone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] At 20°C, take 10 kg of abalone viscera that has been cleaned and removed of connective tissue, add 20 kg of distilled water, beat the pulp, pass through a 40-mesh sieve, and stir well to prepare abalone viscera pretreatment solution, centrifuge, remove sediment, and take Serum. A membrane stack of 40 membrane pairs is used, and the supernatant is subjected to electrodialysis treatment with a voltage of 24V to obtain the light phase. Concentrate the light-phase treatment solution until the soluble solids content reaches 5%, to obtain a concentrated solution, add 95% alcohol according to 2.5 times the volume of the concentrated solution, let it stand for alcohol precipitation for 11 hours, take the supernatant and carry out rectification to recover ethanol, Then concentrate and freeze-dry to obtain 68g of alcohol-soluble antioxidant substances in abalone viscera. After the sample was tested, the heavy metal content was 7.0ppm, which was two-thirds lower than that of the ...

Embodiment 2

[0027] At 30°C, take 10 kg of abalone viscera that has been cleaned and removed of connective tissue, add 30 kg of distilled water, beat the pulp, pass through a 40-mesh sieve, and stir well to prepare abalone viscera pretreatment solution, centrifuge, remove sediment, and take Serum. A membrane stack of 40 membrane pairs is used to conduct electrodialysis treatment with a voltage of 32V to obtain the light phase. Concentrate the light-phase treatment solution until the soluble solids content reaches 7%, to obtain a concentrated solution, add 95% ethanol with a purity of 3 times the volume of the concentrated solution, let it stand for alcohol precipitation for 10 hours, take the supernatant and carry out rectification to recover ethanol, Then concentrate and freeze-dry to obtain 72g of alcohol-soluble antioxidant substances in abalone viscera. The sample was tested, and the heavy metal content was 5.0ppm, which was three quarters lower than that of the comparative example. ...

Embodiment 3

[0029] At 20°C, take 10 kg of abalone viscera that has been cleaned and removed of connective tissue, add 40 kg of distilled water, beat the pulp, pass through a 40-mesh sieve, and stir well to prepare abalone viscera pretreatment solution, centrifuge, remove sediment, and take supernatant. A membrane stack of 40 membrane pairs is used to conduct electrodialysis treatment with a voltage of 40V to obtain the light phase. Concentrate the light-phase treatment solution until the soluble solids content reaches 9%, to obtain a concentrated solution, add alcohol with a purity of more than 95% according to 3.5 times the volume of the concentrated solution, let stand for alcohol precipitation for 9 hours, and take the supernatant for rectification to recover ethanol , and then concentrated and freeze-dried to obtain 66 g of alcohol-soluble antioxidant substances in abalone viscera. The sample was tested, and the heavy metal content was 5.0ppm, which was three quarters lower than that...

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Abstract

The invention relates to a method for extracting alcohol-soluble antioxidant substances from the visceral organs of an abalone, belonging to the technical field of extraction of antioxidant substances in the visceral organs of the abalone. The method for extracting comprises the steps of: pulping the visceral organs of the abalone, soaking and extracting, removing heavy metals, concentrating, conducting alcohol precipitation, rectifying, concentrating and drying to obtain the alcohol-soluble antioxidant substances from the visceral organs of the abalone. The alcohol-soluble antioxidant substances in the visceral organs of the abalone related by the invention contain rich protein, amino acid, saccharides, lipid and the like, have good antioxidant activity, and can be used for preparing amino acid supplemented nourishments and various antioxidant preparations.

Description

technical field [0001] The invention relates to the technical field of extracting alcohol-soluble antioxidant substances from abalone viscera. Background technique [0002] Since Denham Harman proposed the free radical theory of body aging, the view that human fatigue, aging and many diseases are related to free radicals produced by oxidation in the body has been more and more recognized, and the research on antioxidants has gradually attracted people's attention. Most of the antioxidants sold in the market are synthetic antioxidants, such as tert-butyl hydroxyanisole (BHA), di-tert-butyl hydroxytoluene (BHT), tert-butyl hydroquinone (TBHQ), although their antioxidant capacity It should not be underestimated, but it has toxic and side effects, will bring negative effects to the body, and has many hazards lurking in the human body. Such antioxidants cannot satisfy the public's pursuit of health concepts. Therefore, it is necessary to find and develop safe, non-toxic and Nat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/56A23L1/29A61P39/06A23L33/00A61K35/618
Inventor 方婷陈锦权孙沈鲁苏柳智
Owner FUJIAN AGRI & FORESTRY UNIV
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