Paenibacillus polymyxa and application thereof in prevention and treatment of various soil-borne diseases
A polymyxa spore-like and bacillus technology is applied to a strain of polymyxa spore-like bacillus and its application field in preventing and treating various soil-borne diseases
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Embodiment 1
[0085] Example 1. Isolation, screening and identification of Paenibacillus polymyxa ZF129
[0086] 1. Isolation of strains
[0087] 60 bacterial strains were isolated from potato rhizosphere soil in Guyuan, Hebei. The separation method is as follows:
[0088] Weigh 10g of potato rhizosphere soil, dissolve it in 90mL of sterile water, shake it at 28°C and 160r / min for 30min, and mix it well. After the mixed soil samples were bathed in 80°C water for 10 minutes, according to 10 -2 to 10 -5 Perform gradient dilution, draw 100 μL of the diluted suspension to coat LB solid plates, coat 3 plates for each concentration, and culture at a constant temperature of 28°C for 2 days, pick bacterial colonies with different shapes, streak the plates, purify them, and store them in numbers.
[0089] 2. Strain screening
[0090] The plate confrontation method was used to measure the inhibitory rate of the 60 strains isolated in step 1 to the potato black mole pathogen Rhizoctonia solani. ...
Embodiment 2
[0109] Embodiment 2, Paenibacillus polymyxa ZF129 antibacterial spectrum determination
[0110] 1. Determination of fungal inhibition spectrum
[0111] According to the method in embodiment 1 step 2, Paenibacillus polymyxa ZF129 is detected to Corynesium polymyxa, Fusarium oxysporum, Capsicum anthracnose, Phytophthora capsicum, Verticillium dahliae, Alternaria solani, Gray Inhibition rates of Botrytis and Rhizoctonia solani.
[0112] Corynespora cassiicola has been described in the literature "Gao Wei, Li Baoju, Shi Yanxia, Xie Xuewen. Differentiation of pathogenicity of Corynespora on cucumber, tomato and eggplant hosts. Journal of Horticultural Science, 2011, 38(3) : 465-470.", and the public can obtain it from the Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, to repeat the experiment of this application, and cannot be used for other purposes.
[0113] Fusarium oxysporum has been reported in the literature "Shi Yanxia, Zhang Xiaohui, Xu...
Embodiment 3
[0131] Embodiment 3, Paenibacillus polymyxa ZF129 different medium fermented liquid is to the inhibitory effect of potato fusarium wilt bacterium growth
[0132] 1. Paenibacillus polymyxa ZF129 was inoculated in LB liquid medium, and incubated at 28° C. and 180 r / min for 16 hours to obtain mother liquor.
[0133] 2. Inoculate the mother liquor prepared in step 1 into the culture medium at a ratio of 1:20 (volume ratio), and incubate at a constant temperature of 28°C and 180r / min for 24h.
[0134] The medium is soybean flour 8 medium, cottonseed cake medium, corn fishmeal medium, soybean meal medium, mannitol medium and corn flour medium.
[0135] Soybean flour 8 medium: soybean cake powder 30g, peptone 2g, potassium dihydrogen phosphate KH 2 PO 4 0.2g, calcium carbonate CaCO 3 1g, glucose 5g, yeast powder 3g, distilled water 1000mL.
[0136] Cottonseed cake medium: cottonseed cake powder 32.5g, corn flour 14g, bran 12g, potassium dihydrogen phosphate KH 2 PO 4 1.1g, ir...
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