Paenibacillus polymyxa and application thereof in prevention and treatment of various soil-borne diseases

A polymyxa spore-like and bacillus technology is applied to a strain of polymyxa spore-like bacillus and its application field in preventing and treating various soil-borne diseases

Active Publication Date: 2021-08-13
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are very few reports on the broad-spectr

Method used

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  • Paenibacillus polymyxa and application thereof in prevention and treatment of various soil-borne diseases
  • Paenibacillus polymyxa and application thereof in prevention and treatment of various soil-borne diseases
  • Paenibacillus polymyxa and application thereof in prevention and treatment of various soil-borne diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Example 1. Isolation, screening and identification of Paenibacillus polymyxa ZF129

[0086] 1. Isolation of strains

[0087] 60 bacterial strains were isolated from potato rhizosphere soil in Guyuan, Hebei. The separation method is as follows:

[0088] Weigh 10g of potato rhizosphere soil, dissolve it in 90mL of sterile water, shake it at 28°C and 160r / min for 30min, and mix it well. After the mixed soil samples were bathed in 80°C water for 10 minutes, according to 10 -2 to 10 -5 Perform gradient dilution, draw 100 μL of the diluted suspension to coat LB solid plates, coat 3 plates for each concentration, and culture at a constant temperature of 28°C for 2 days, pick bacterial colonies with different shapes, streak the plates, purify them, and store them in numbers.

[0089] 2. Strain screening

[0090] The plate confrontation method was used to measure the inhibitory rate of the 60 strains isolated in step 1 to the potato black mole pathogen Rhizoctonia solani. ...

Embodiment 2

[0109] Embodiment 2, Paenibacillus polymyxa ZF129 antibacterial spectrum determination

[0110] 1. Determination of fungal inhibition spectrum

[0111] According to the method in embodiment 1 step 2, Paenibacillus polymyxa ZF129 is detected to Corynesium polymyxa, Fusarium oxysporum, Capsicum anthracnose, Phytophthora capsicum, Verticillium dahliae, Alternaria solani, Gray Inhibition rates of Botrytis and Rhizoctonia solani.

[0112] Corynespora cassiicola has been described in the literature "Gao Wei, Li Baoju, Shi Yanxia, ​​Xie Xuewen. Differentiation of pathogenicity of Corynespora on cucumber, tomato and eggplant hosts. Journal of Horticultural Science, 2011, 38(3) : 465-470.", and the public can obtain it from the Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, to repeat the experiment of this application, and cannot be used for other purposes.

[0113] Fusarium oxysporum has been reported in the literature "Shi Yanxia, ​​Zhang Xiaohui, Xu...

Embodiment 3

[0131] Embodiment 3, Paenibacillus polymyxa ZF129 different medium fermented liquid is to the inhibitory effect of potato fusarium wilt bacterium growth

[0132] 1. Paenibacillus polymyxa ZF129 was inoculated in LB liquid medium, and incubated at 28° C. and 180 r / min for 16 hours to obtain mother liquor.

[0133] 2. Inoculate the mother liquor prepared in step 1 into the culture medium at a ratio of 1:20 (volume ratio), and incubate at a constant temperature of 28°C and 180r / min for 24h.

[0134] The medium is soybean flour 8 medium, cottonseed cake medium, corn fishmeal medium, soybean meal medium, mannitol medium and corn flour medium.

[0135] Soybean flour 8 medium: soybean cake powder 30g, peptone 2g, potassium dihydrogen phosphate KH 2 PO 4 0.2g, calcium carbonate CaCO 3 1g, glucose 5g, yeast powder 3g, distilled water 1000mL.

[0136] Cottonseed cake medium: cottonseed cake powder 32.5g, corn flour 14g, bran 12g, potassium dihydrogen phosphate KH 2 PO 4 1.1g, ir...

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Abstract

The invention discloses a paenibacillus polymyxa and application thereof in prevention and treatment of various soil-borne diseases. The preservation number of the paenibacillus polymyxa ZF129 protected by the invention is CGMCC (China General Microbiological Culture Collection Center) No.17631. The paenibacillus polymyxa ZF129 can remarkably inhibit the growth of pathogenic fungus hyphae, shows broad-spectrum antagonistic activity, and has the capability of promoting the growth of plants. The paenibacillus polymyxa ZF129 shows an excellent biocontrol character, has a further development potential, and lays a certain foundation for further study of the paenibacillus on the mechanism of disease prevention.

Description

technical field [0001] The invention relates to a strain of Paenibacillus polymyxa and its application in preventing and treating various soil-borne diseases. Background technique [0002] Soil-borne diseases have become a worldwide problem, endangering the sustainable development of agriculture. Rhizoctonia solani, Verticillium dahliae, Phytophthora capsici, and Plasmodium brassicae are common soil-borne pathogens. They have a wide range of hosts, causing a large amount of crop yield reduction and serious economic losses. At present, due to intensive planting, single continuous cropping and other production modes, soil-borne diseases have rapidly become an important factor limiting crop yield and quality. In addition, the spread of soil-borne diseases makes crop production more and more dependent on pesticides, which in turn causes environmental pollution and food safety issues. Therefore, it is particularly important and urgent to carry out research on biological control...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P1/00A01P3/00C12R1/12
CPCC12N1/20A01N63/00
Inventor 李磊李宝聚赵昱榕谢学文石延霞柴阿丽
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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