Magnetic immunochromatographic strip for detection of tuberculosis (TB) antibody in blood and preparation method thereof

A technology of magnetic immunochromatography and test strips, which is applied in the field of medical testing, can solve the problems of long reaction time, low sensitivity, and inability to detect a single person, and achieve the effect of easy operation

Inactive Publication Date: 2010-06-30
CHEMCLIN DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Serological testing can detect asymptomatic infections early, but ELISA test procedures are complex, prone to false positive or false negative results, and low sensitivity. CLIA is similar to ELISA, but the sensitivity is improved, and it does not fundamentally solve the problem of ELISA. problems, and both of them have the problems of cumbersome operation and long reaction time, and both require complex equipment such as microplate reader or luminometer, plate washer and incubator, and cannot be tested by a single person, which further limits their use in some Application in primary hospitals and clinics
In recent years, rapid detection test strips represented by colloidal gold or latex particles have appeared at home and abroad, but because the results are visually observed by the naked eye, they are easily affected by the subjective judgment of the observer, and the sensitivity is low, and the accuracy of the results is not high.

Method used

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  • Magnetic immunochromatographic strip for detection of tuberculosis (TB) antibody in blood and preparation method thereof
  • Magnetic immunochromatographic strip for detection of tuberculosis (TB) antibody in blood and preparation method thereof

Examples

Experimental program
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Embodiment 1

[0029] Preparation method of magnetic immunochromatography test strip and test paper box for detecting TB antibody in blood

[0030] The preparation method of test strip and test paper box of the present embodiment comprises the following steps:

[0031] A. Preparation of antigen and antibody: commercialized TB recombinant antigen was selected, dialyzed overnight at 4°C against 20 mM PBS with pH 7.2 (pH 7.0-7.6 is applicable), and used for later use.

[0032] B. Preparation of coating film:

[0033] Coating buffer preparation: 0.02M phosphate buffer solution (PBS) with pH 7.2 was used as the coating buffer, which was sterilized by 0.22 μm microporous membrane and stored at 4°C for later use, with a validity period of two weeks.

[0034] Preparation of blocking solution: 0.02M phosphate buffered saline (PBS) containing 0.5% BSA at pH7.2 (pH7.0-7.6 is applicable), 0.22μm microporous membrane filter and sterilize, and store at 4°C for later use. Valid for one week.

[0035] Pr...

Embodiment 2

[0058] In the steps except the preparation of magnetic particles: the molecular ratio of TB antigen to magnetic particles is 2:1. Other steps are with embodiment 1,

Embodiment 3

[0060] In the steps except the preparation of magnetic particles: the molecular ratio of TB antigen to magnetic particles is 10:1. Other steps are the same as in Example 1.

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Abstract

The invention relates to a magnetic immunochromatographic strip for detection of tuberculosis (TB) antibodies in blood and a preparation method thereof. The strip is prepared through the following steps: pasting a coating film, a magnetic particle pad combined with TB antigens, a sample pad and an absorbent pad on a bottom board in sequence at intervals of 2mm, and using a transparent plastic seal film to cover the upper layer. The coating film is pre-coated with TB antigen detection lines and quality control lines. The invention introduces the magnetic immunochromatographic technique into the detection of the TB antibody, thereby greatly improving the detection sensitivity and accuracy, shortening the detection window period and reducing the labor intensity of the operating personnel.

Description

technical field [0001] The invention relates to a magnetic immunochromatographic test strip for detecting TB antibody and a preparation method thereof, which relate to the field of medical examination. Background technique [0002] Tuberculosis (TB) is a common and fatal infectious disease caused by mycobacteria (mainly Mycobacterium tuberculosis, also known as tubercle bacillus). Tuberculosis usually infects and destroys the lungs (called "pulmonary tuberculosis", also known as "pulmonary tuberculosis") and the lymphatic system (called "tuberculous lymphatic disease", also known as "lymphatic tuberculosis"), but other organs such as the brain, central nervous system, circulatory system , urinary system, bones, joints, and even the skin can also be infected (for example, infection of the brain can cause "tuberculous meningitis"). Other mycobacteria, such as Mycobacterium bovis, Mycobacterium africanum, Mycobacterium canetti, Mycobacterium microti, can also cause tuberculosi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/532G01N33/569
Inventor 姚洪涛应希堂李强胡国茂郑金来张坤
Owner CHEMCLIN DIAGNOSTICS CO LTD
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