Recombinant adenovirus expressing Asian type 1 foot-and-mouth disease virus vlp and its application

Abstract

The invention discloses a recombinant adenovirus expressing Asian type 1 foot-and-mouth disease virus VLP and application thereof. The present invention splices the FMDV capsid protein precursor P1-2A gene and 3C gene together to obtain a fusion gene, and its base sequence is shown in SEQ ID NO:1. The base sequence shown in SEQ ID NO: 1 is connected with the adenovirus shuttle vector to obtain a recombinant adenovirus shuttle vector; the recombinant adenovirus shuttle vector is recombined into human defective adenovirus type 5, screened and identified to obtain a highly efficient The recombinant adenovirus expressing Asian type 1 foot-and-mouth disease virus VLP, its microorganism preservation number is: CGMCC No.3467. The Asian type 1 foot-and-mouth disease virus VLP expressed by the defective adenovirus constructed by the present invention can continuously induce high levels of neutralizing antibodies in mice, and can be used as a vaccine for preventing Asia 1 type foot-and-mouth disease.

Description

technical field [0001] The present invention relates to a recombinant adenovirus, in particular to a recombinant adenovirus expressing Asian type 1 foot-and-mouth disease virus VLP. The present invention also relates to the construction method of the recombinant adenovirus and the application of the recombinant adenovirus in the prevention of foot-and-mouth disease, belonging to foot-and-mouth disease the field of prevention. Background technique [0002] Foot-and-mouth disease (FMD) is an acute, febrile, highly contagious infectious disease of cloven-hoofed animals, and its pathogen is foot-and-mouth disease virus (FMDV). In view of the serious harm caused by foot-and-mouth disease to the world economy, the International Office of Epizootics (OIE) and the United Nations Food and Agriculture Organization (FAO) listed it as the first class A infectious disease, and my country also listed it as the first class of animal infectious diseases. Vaccination is an effective means t...

AI Technical Summary

Problems solved by technology

Because FMDV is a highly mutated virus, the nucleotide sequences of different serotypes such as O-type and Asia1-type foot-and-mouth disease differ by more than 15%. In addition, the genome structure of FMDV is unique, and it is very difficult to clone and assemble correctly. It is technically difficult to express FMD virus-like particles and induce high levels of neutralizing antibodies in animals

Images