Method for quantitatively testing activity of microalgae

A microalgae and activity technology, applied in the field of quantitative determination of microalgae activity, can solve the problems of inability to effectively reflect the information of microalgae activity, large errors in salinity in algae, etc.

Inactive Publication Date: 2010-09-01
BEIHANG UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the dry weight of algae cells is the most direct and reliable indicator, but in the high-density culture system, the error caused by the salinity in the algae is very large, and the dry weight of algae cells cannot effectively reflect the information about the activity of microalgae in the culture system

Method used

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  • Method for quantitatively testing activity of microalgae

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Embodiment Construction

[0008] The specific implementation manners of the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0009] 1) Take an appropriate amount of the microalgae solution to be tested from the microalgae culture system, and aerate for 5 minutes to make the CO in the solution 2 The concentration is close to saturation;

[0010] 2) Add the microalgae solution to be tested through sufficient aeration in the iodine measuring bottle 4 at once, and put into the stirrer 2, plug the iodine measuring bottle with the holed bottle stopper 5 that is inserted with the dissolved oxygen meter probe 6 immediately, to avoid Air leakage, allowing a small amount of algae liquid to overflow at this time to prevent air bubbles in the bottle;

[0011] 3) Turn on the light source 3, adjust its light intensity to be consistent with the light intensity of the light source in the microalgae culture system, adjust the stirring intensity of the stirrer...

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Abstract

The invention relates to a method for quantitatively testing the activity of microalgae, which is a characterization method created mainly for solving the problem of the distortion of conventional growth condition indexes of the microalgae in the high-density culture of the microalgae. A device consists of a magnetic stirring apparatus, a stirrer, a light source, an iodine number flask, a plug with holes, a dissolved oxygen probe and a dissolved oxygen tester, and is utilized to continuously test the concentration of microalgae suspension dissolved oxygen in the iodine number flask, calculate the amount of oxygen released by photosynthesis from the microalgae in a culture system and then calculate a specific photosynthetic oxygen evolution speed of the microalgae according to the biomass concentration of the microalgae to represent the growth condition of the microalgae.

Description

technical field [0001] The invention relates to a method for quantitatively measuring the activity of microalgae, which is mainly aimed at the distortion problem of the traditional indicators of the growth status in the high-density culture of microalgae, and is established to characterize the activity of microalgae in the microalgae culture system. Growth Status. Background technique [0002] Because of its high oil content, easy cultivation, short growth cycle, and large output per unit area, microalgae are regarded as a new generation, or even the only biodiesel raw material that can completely replace petrochemical diesel. For the microalgae biodiesel technology industry For the smooth progress of microalgae cultivation, it is necessary to establish a large-scale, low-cost and high-efficiency culture system for microalgae, and it is particularly important to monitor the growth status of microalgae in the system. [0003] There are many indicators to quantify the growth ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/00
Inventor 刘红李明
Owner BEIHANG UNIV
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