Method for removing hemolytic toxicity from physaliatoxin

A toxin solution and toxicity technology, applied in the field of medicine, to achieve the effect of simple and effective detoxification of hemolysis with simple method

Inactive Publication Date: 2012-02-01
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no relevant report on the removal of high-abundance hemolytic toxic components in jellyfish crude venom by alkaline conditions

Method used

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  • Method for removing hemolytic toxicity from physaliatoxin
  • Method for removing hemolytic toxicity from physaliatoxin

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Experimental program
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Embodiment 1

[0018] Embodiment 1. Preparation removes hemolytic toxicity jellyfish toxin

[0019] 1. Preparation of jellyfish crude venom extract

[0020] Faxingxia jellyfish was collected from the sea area of ​​Sanmen Bay, Zhejiang Province. Take 10g of tentacles and add self-prepared seawater (NaCl 28g, MgCl 2 ·6H 2 O 5g, KCl 0.8g, CaCl 2 1.033g, add water to 1000ml) 10ml, autolyze for 4 days, stir twice a day with a magnetic stirrer, 10min each time. Filter 3 times with a 100-mesh cell sieve, centrifuge the filtrate 3 times at 10,000 g for 15 min each time, and collect the supernatant, which is the crude tentacle-only extract of jellyfish (cTOE, crude tentacle-only extract). Preparation of detoxified jellyfish toxin

[0021] Use 0.1mol / L NaOH to adjust the pH of the jellyfish crude poison extract to 10.5, let it stand for 4h, centrifuge at 10000g for 15min to remove the precipitate, and take the supernatant; mol / L, pH 6.0 acetate buffer solution for 24 hours, then the buffer solut...

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Abstract

The invention relates to the technical field of medicines, in particular to a method for removing hemolytic toxicity from physaliatoxin. The method includes the steps that: (1) jellyfish tentacles are autolyzed in self-prepared seawater with the temperature of 4 DEG C for four days, a magnetic stirrer carries out stirring, tentacle-autolyzed liquid is collected, and is centrifugated under 10000g for 15 minutes, and supernate is crude jellyfish toxin extract; (2) the pH of the crude jellyfish toxin extract is regulated to 10.5 by 0.1mol / L of NaOH, the crude jellyfish toxin extract is kept still for 4 hours, and is centrifugated under 10000g for 15 minutes to remove precipitate, the supernate is dialyzed by a dialysis bag, the permeable molecular weight of which is 10kDa, 0.02mol / L of acetic acid buffer solution with the pH of 6.0 is first used for 24-hour dialysis, buffer solution is then replaced by 0.02mol / L of Tris-acetic acid buffer solution with the pH of 9.5 to continue dialysis for 24 hours, centrifugation is carried out under 10000g for 15 minutes to remove precipitate, and supernate is physaliatoxin soilution without hemolytic toxicity. The invention provides a simple and effective method for removing hemolytic toxicity from physaliatoxin.

Description

technical field [0001] The invention relates to the technical field of medicine, and relates to a method for removing hemolytic toxicity of jellyfish toxin. Background technique [0002] Jellyfish toxin is a class of peptide toxins with novel structure and strong toxicity, which has many biological activities such as cardiovascular, hemolytic, nervous and respiratory. Due to the characteristics of temperature sensitivity, instability, easy adhesion, and easy inactivation of jellyfish toxins, the purification and identification of its toxic components are progressing slowly, generally lagging behind other common biological toxins. [0003] Cardiovascular toxicity and hemolytic toxicity of jellyfish toxins play an important role in jellyfish sting poisoning, and they are also the two most reported toxic components in jellyfish toxins. Among them, the abundance of cardiovascular toxic components is low, but the cardiovascular function damage caused by jellyfish toxins is the m...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): A61K38/17A61K35/614
Inventor肖良王倩倩张黎明刘四化
OwnerSECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY