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Individual fluorescence labeling method for early-stage newborn green crabs to adult green crabs

A technology for fluorescent labeling and blue crabs, which is applied in the field of individual labeling of crabs, individual fluorescent labeling of early larvae and adult crabs of blue crabs, and achieves the effects of wide application range, convenient identification and simple operation

Inactive Publication Date: 2010-12-15
XIAMEN UNIV
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

In addition, after the injection of fluorescent colloid, whether the fluorescent colloid enters the organism, whether it will affect the growth and development of the organism, has not yet been reported.

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] The technology of the present invention has been repeatedly implemented for many times, and the effect is very good, and the detailed description is as follows:

[0019] 1. Prepare the fluorescent gel label, and put the fluorescent gel label into the syringe for later use.

[0020] 2. Keep the blue crabs temporarily in a pool for 2 to 3 days before anesthesia. Place the scylla crab in ice water at 0~4℃ to reduce its activity before injecting the fluorescent colloidal label. When the scylla’s shell width is 1.5~5.0cm, the low-temperature anesthesia time before the fluorescent colloidal label injection is 6-8s; At 5.0cm, the hypothermic anesthesia time before the injection of the fluorescent gel label is 10-12s.

[0021] 3. Injection of fluorescent gel label:

[0022] 1) When the shell width of the scylla is 1.5~5.0cm, use a 0.3ml syringe to mark. The needle is inserted from the base segment and the membrane joint of the base segment and into the second, third and fourth pairs o...

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PUM

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Abstract

The invention discloses an individual fluorescence labeling method for early-stage newborn green crabs to adult green crabs and relates to an individual labeling method used in aquiculture. The method comprises the following steps of: preparing a fluorescent colloid label and putting the fluorescent colloid label into a syringe for later use; before injecting the fluorescent colloid label, putting the green crabs in ice water with the temperature of between 0 and 4 DEG C to lower the activities of the green crabs; in the process of injecting the fluorescent colloid label, inserting a syringe needle into a part on which the head, chest and belly cortex of the green crab is jointed with muscle from the bottom end of an appendage of the green crab along a belly wall and extracting the syringe needle outwards to form a linear label; and after the fluorescent colloid label is injected, wiping the injected fluorescent colloid label remaining around a position where the syringe needle is inserted.

Description

Technical field [0001] The present invention relates to an individual marking method in aquaculture, in particular to a method for fluorescent marking individual crabs from early larvae to adult crabs, which is suitable for crab individual marking in aquatic breeding and release, genetic breeding and family selection . Background technique [0002] Individual markers are commonly used techniques in marine fishery resource surveys and aquatic genetic breeding. They are often used in fishery proliferation and release, population dynamics studies, and family markers in genetic breeding. At present, a variety of individual marking techniques have been applied to aquatic products, but there are few individual marking techniques that can be used on crabs. This is because early crabs have small individuals, fragile bodies, and high molting frequency, which limit Many applications of individual marking technology. For example: the use of branding technology will cause the death of frag...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 王桂忠刘子明叶海辉李少菁陶永陈学雷
Owner XIAMEN UNIV
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