Brucella detection kit and using method thereof

A detection kit and technology for Brucella, which is applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as long detection cycle, high cost of fluorescent probes, and complicated procedures
CN101979660AActive Publication Date: 2011-02-23GUANGZHOU HUAFENG BIOTECH +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGZHOU HUAFENG BIOTECH
Publication Date
2011-02-23

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Abstract

The invention provides a brucella detection kit. The brucella detection kit comprises two pairs of primers, namely internal primers FIP / BIP and external primers F3 / B3 which take the OMP25 gene of brucella as a target gene and are designed based on loop-mediated isothermal amplification technology. The brucella detection kit has more comprehensive detection effect and low undetected rate.
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Description

technical field

[0001] The invention relates to a biological detection reagent, in particular to a Brucella detection kit and a use method thereof. Background technique

[0002] Brucella is a Gram-negative, non-motile bacterium that can survive as an intracellular parasite in many species of livestock. The principal cause of infection in humans is Brucella malta ("Maltese fever"), abortive cloth Bacillus, Brucella bovine, sheep, Brucella suis, and Brucella canis. After a person is infected, the first symptom is fever, the body temperature can reach 38-40 degrees, and it lasts for a long time, and it is in a long-term low-grade state; , Repeated many times, so brucellosis is also called undulant fever. Therefore, it is of great significance to detect Brucella accurately and rapidly.

[0003] Traditional Brucella detection methods are far from meeting the requirements of modern detection due to their shortcomings such as long detection cycle, complicated procedures, and var...

Claims

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