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Gene chip for detecting pancreatic cancer and application thereof

A technology of gene chip and pancreatic cancer, applied in the field of gene chip for detecting pancreatic cancer, can solve problems such as poor effect, and achieve the effect of improving the cure rate, good clinical application prospect, and reducing the mortality rate

Inactive Publication Date: 2012-09-05
SHANGHAI EAST HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, immunological diagnostic products for pancreatic cancer and PCR products have the disadvantage of not being able to balance the detection sensitivity and specificity of a single marker. In view of the poor effect of the current traditional methods for detecting pancreatic cancer, it is necessary to use gene chip technology to develop a method for detecting pancreatic cancer. The gene chip can be effectively diagnosed in the early stage of pancreatic cancer development, so as to improve the cure rate and reduce the mortality rate

Method used

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  • Gene chip for detecting pancreatic cancer and application thereof
  • Gene chip for detecting pancreatic cancer and application thereof
  • Gene chip for detecting pancreatic cancer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 3

[0050] Embodiment 3 chip hybridization experiment:

[0051] 1. Total RNA extraction (TRIzol method):

[0052] Add 1ml Trizol (purchased from SIGMA Company) per 100mg of tissue, and use a homogenizer to fully break up. Add about 1 / 5 volume of chloroform, mix thoroughly and let stand at room temperature for 5 minutes. After centrifugation at 12,000rpm at 4°C for 15 minutes, transfer the supernatant to a new 1.5ml centrifuge tube, add an equal volume of isopropanol, gently invert and mix, and let stand at room temperature for 5 minutes. After centrifugation at 12000 rpm at 4°C for 10 minutes, the supernatant was removed, 2 / 5 volume of 70% ethanol was added to the precipitate, and the precipitate was washed by centrifugation at 12000 rpm at 4°C for 15 minutes. Remove the supernatant, dry the precipitate at room temperature, add an appropriate amount of RNase-free water to fully dissolve the precipitate, and measure the OD260 and OD280 values.

[0053] 2. Total RNA quality detec...

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Abstract

The invention discloses a gene chip for detecting pancreatic cancer, comprising a solid phase vector and a probe, wherein the probe is hybridized with nucleotide sequences of related pancreatic cancer genes to be detected and / or complementary sequences of the nucleotide sequences; the related pancreatic cancer genes to be detected comprise P21, smad4, P16, c-Myc, bc1-2, Fas, CA19-9, SERPINB9, RHOT1 and P53 gene. The invention further discloses a method for detecting the pancreatic cancer by applying the gene chip. The gene chip of the invention can be used for performing effective detection at the early period of generation and development of the pancreatic cancer so as to improve cure rate and reduce mortality rate.

Description

technical field [0001] The invention relates to a gene chip, in particular to a gene chip for detecting pancreatic cancer and its application. Background technique [0002] Pancreatic cancer is one of the common malignant tumors. In recent years, its incidence has been on the rise, and there is a lack of effective diagnostic methods and treatment measures. As a result, pancreatic cancer patients are found late, with poor prognosis and high mortality. Regular screening of ordinary and high-risk groups is one of the effective measures to improve the early diagnosis rate and detect diseases in time. [0003] In fact, the development of pancreatic cancer involves changes in the expression of multiple genes. If the cancer is diagnosed at the gene level in the early stage of pancreatic cancer, and the expression changes of important related genes are detected, it will be beneficial to the early detection and early treatment of cancer and improve the survival rate of patients. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C40B40/06
Inventor 江华
Owner SHANGHAI EAST HOSPITAL
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