Fusion protein MBP-NAP and preparation method and application thereof

A fusion protein, hp-napa technology, applied in the field of cell biology and genetic engineering, to achieve the effect of efficient fusion expression

Inactive Publication Date: 2011-04-06
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the patent of using HP-NAP to modify the fusion protein to enhance Th1 cell immunity has not been reported at home and abroad.

Method used

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  • Fusion protein MBP-NAP and preparation method and application thereof
  • Fusion protein MBP-NAP and preparation method and application thereof
  • Fusion protein MBP-NAP and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0036] The present invention will be further described below through specific embodiments, but the protection scope of the present invention is not limited thereto.

[0037] Preparation of a fusion protein:

[0038] (1) PCR amplification of gene HP-napA

[0039]According to the complete genome sequences of H.pylori 26695 and H.pylori J99 published on the website of the National Center for Biotechnology Information (NCBI), PCR primers were designed, and a restriction end was introduced at the 5′ end of the upstream primer. Dicer EcoR I restriction site (underlined part) and protective base GGA, retain the initiation codon ATG; introduce Sal I restriction site (underlined part) and protective base AA at the 5' end of the downstream primer, The stop codon TAA was retained. The primer sequences are as follows:

[0040] Upstream primer P1: 5′-G GAGAATTC ATGAAAACATTTGAAAT-3′

[0041] Downstream primer P2: 5′-AA GTC GAC TTAAGCTAAATGGGCTTCTAG-3′

[0042] Primers were synthesi...

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PUM

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Abstract

The invention relates to a fusion protein MBP-NAP. The amino acid sequence of the fusion protein is shown as SEEQ ID NO.1. Moreover, the invention also discloses a preparation method of the fusion protein MBP-NAP. The method has the characteristics that: the method is easy and convenient to operate, has a mild condition and the like. Th1 cellular immunity of the fusion protein MBP-NAP of the invention is enhanced, so that the secretory volumes of related cell factors IL-2 and IFN gamma are increased remarkably and Th1 immunity is enhanced.

Description

technical field [0001] The invention belongs to the fields of cell biology and genetic engineering, and specifically relates to a fusion protein MBP-NAP, and also relates to a preparation method of the fusion protein and its application in enhancing Th1 cell immunity. Background technique [0002] According to the cytokine secretion pattern, CD4 + T cells can be divided into Th1 and Th2 subsets, and the balance of Th1 / Th2 cells plays a key role in the regulation of the entire immune system. Th1 and Th2 have antagonistic effects, and an imbalance between them can lead to different types of diseases. Studies have found that Th1 / Th2 balance disorders are related to various diseases such as tumors, autoimmune diseases, microbial infections, and transplant rejection. Since Yamamura and Kharkevitch first discovered that the immune response shifted to Th2 when tumors occurred, many studies have confirmed that the peripheral blood of patients with advanced tumors is often in a Th2...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C12N15/62C12N15/70A61K38/17A61P37/04
Inventor 康巧珍段广才汲振余周晴晴刘伟高艳锋祁元明
Owner ZHENGZHOU UNIV
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