Method and device for breaking down biological material

A biomaterial and biodecomposition technology, applied in the field of obtaining biomolecules, can solve the problems of expensive and complicated methods, and achieve the effect of improving the connection

Active Publication Date: 2014-05-14
QIAGEN GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the presented method is complex,

Method used

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  • Method and device for breaking down biological material
  • Method and device for breaking down biological material
  • Method and device for breaking down biological material

Examples

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[0023] Figure 1 to Figure 5 The flow of the method according to the invention is shown.

[0024] figure 1 Container 10 closed with lid 11 is shown. The liquid 12 is located in the container 10 and the material 14 to be decomposed is present in the container 10 . The sonotrode 13 is arranged on the wall section 15 of the container 10 . The container 10 is located in a device (not shown) that generates a centrifugal force 18 directed outwardly, as in figure 2 and 3 as shown. The centrifugal force 18 causes the material 14 to be decomposed to rest at the focal point 16 on the wall section 15 . The material 14 to be decomposed gathers at the focal point 16 . In addition, the ultrasonic waves generated by the generator 13 are also concentrated at the focal point 16, see image 3 , so that a force development (Kraftentfaltung) of the ultrasonic force 19 takes place here, whereby the material 14 to be decomposed is decomposed. At the same time, the non-decomposable or inso...

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Abstract

The invention relates to a method for breaking down biological material, especially for obtaining biomolecules by ultrasound, the biological material being arranged, in a container together with a liquid.

Description

technical field [0001] The present invention relates to a method for decomposing biological material by means of ultrasound, in particular for obtaining biomolecules, and to a device for the method, wherein the biological material is placed in a container together with a liquid. Background technique [0002] Such methods are known from the prior art, for example using ultrasound to decompose materials, eg organic or inorganic sample materials or fossil materials. Decomposition is carried out in this case to obtain the target constituents of the material to be decomposed. When decomposing, a distinction is made between open environment parameters and closed environment parameters. Of these, the decomposition in closed vessels (see, for example, US-A-6100084) is absolutely crucial, since an impurity-free decomposition can thereby be achieved, which is particularly advantageous for the decomposition of biological materials. In this case, the material to be decomposed is in a ...

Claims

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Application Information

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IPC IPC(8): C12M3/08
CPCC12N1/066C12N13/00
Inventor 托马斯·罗特曼克劳迪娅·施米特安德烈亚斯·舍费尔加布里埃莱·克里斯托弗尔
Owner QIAGEN GMBH
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