Method for distinguishing different types of culture media in scale plant tissue culture production

A technology for plant tissue culture and culture medium, applied in the field of distinguishing different types of culture medium, can solve the problems of wrong use of culture medium, culture medium sub-packaging, sterilization, transport, confusion in distribution, and increase the difficulty of cleaning, so as to reduce cleaning Difficulty, the effect of saving work

Inactive Publication Date: 2011-04-20
BEIJING AGRO BIOTECH RES CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The first method is the safest and less prone to errors, but the workload of labeling is heavy, and it also increases the difficulty of cleaning when the culture bottle is used again
The second method is relatively simple, but it is prone to confusion in the process of sterilization, transfer and distribution of multi-variety and multi-type media
[0003] In actual production, because of confusion in any link of subpackaging, sterilization, transfer, and distribution of the medium, it will eventually lead to the wrong use of the medium in the tissue culture process, which has very serious consequences for large-scale tissue culture.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] When producing bananas and papaya test-tube seedlings in large quantities, the types of culture medium include banana proliferation medium, banana rooting medium, papaya proliferation medium, and papaya rooting medium. These four mediums are colorless and transparent solutions It is difficult to distinguish with the naked eye. By not adding pigments and adding three colors of pigments of amaranth, tartrazine and indigo at a concentration of 0.5 parts per million, the four cultures of colorless, red, yellow and blue can be distinguished by the naked eye. The base reduces the workload of manual labeling and avoids the confusion of medium use caused by errors in the process of sterilization, transportation and distribution.

Embodiment 2

[0024] Three kinds of pigments of amaranth, tartrazine and indigo are respectively formulated into pigment concentrates with a weight concentration of 5%, and the solvent is deionized water; One ten-thousandth is added to banana proliferation medium, banana rooting medium, papaya proliferation medium and papaya rooting medium, and then sterilized and stored respectively.

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Abstract

The invention discloses a method for distinguishing different types of culture media in scale plant tissue culture production, which is characterized in that after the culture media are prepared, one or more than one water-solubility and high temperature and pressure resistant artificially synthesized edible pigment is added to the culture media. In the invention, through adding one or more than one high temperature and pressure resistant artificially synthesized edible pigment with good water solubility to different types of culture media in the culture media preparation stage, the types of the culture media can be distinguished by naked eyes, and the culture media use error and chaos caused by the reason that marks are unclear or lost in the sterilization, transfer and distribution process are avoided, thereby not only leaving out the working link of marking the culture media but also lowering the cleaning difficulty when a culture container is reutilized.

Description

technical field [0001] The invention relates to a method for distinguishing different kinds of culture media, in particular to a method for distinguishing different kinds of culture media in large-scale plant tissue culture production. Background technique [0002] Large-scale multi-variety plant tissue culture production in enterprises often involves multiple plant species, and different plant species require different types of media at different stages. In the actual production process, the preparation of the medium is usually uniformly prepared, sterilized uniformly, and then distributed to operators for different types of operations and cultivation at different stages. In this process, there is a problem of distinguishing and distributing different types of media. There are two traditional methods to distinguish the types of culture media: the first is to mark the types of media on all tissue culture containers; the second is to mark the entire batch of the same media u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00G09F7/00
Inventor 邢礼军苏炳豪马荣才苏坚宏王旭明魏建华
Owner BEIJING AGRO BIOTECH RES CENT
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