Dual SYBR Green I real-time fluorescence PCR detection primer and method for porcine pseudorabies virus and porcine circovirus type 2

A technology of porcine pseudorabies virus and porcine circovirus, applied in the direction of microorganism-based methods, biochemical equipment and methods, fluorescence/phosphorescence, etc., can solve the problems of subclinical infection and no unified diagnostic method, and achieve good sensitivity , Conducive to the effect of identification and diagnosis
CN102071256AInactive Publication Date: 2011-05-25HENAN AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HENAN AGRICULTURAL UNIVERSITY
Publication Date
2011-05-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a dual SYBR Green I real-time fluorescence polymerase chain reaction (PCR) detection primer and a dual SYBR Green I real-time fluorescence PCR detection method for porcine pseudorabies virus and porcine circovirus type 2. The primer is designed and synthesized, and the dual SYBR Green I real-time fluorescence PCR detection method for the porcine pseudorabies virus and the porcine circovirus type 2 by using the primer comprises the following steps of: extracting deoxyribose nucleic acid (DNA) of a sample; and detecting the sample by using an SYBR Green I real-time fluorescence PCR reaction system and an SYBR Green I real-time fluorescence PCR amplification program. By the primer and the method, two viruses, namely the porcine pseudorabies virus and the porcine circovirus type 2 can be detected simultaneously, and porcine parvovirus, classical swine fever virus, porcine reproductive and respiratory syndrome virus and swine influenza virus cannot be detected. The primer and the method have the characteristics of higher sensitivity, repeatability and stability, and contribute to the identification and the diagnosis of pregnant sow reproductive disturbance virus disease.
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Description

technical field

[0001] The invention relates to a real-time fluorescent PCR detection method, in particular to primers and a method for double SYBR Green I real-time fluorescent PCR detection of porcine pseudorabies virus and porcine circovirus type 2. Background technique

[0002] Infection source and natural reservoir host of porcine pseudorabies virus (PRV). Pigs infected with PRV often cause disease in newborn piglets, and the mortality rate can reach 100%. Porcine pseudorabies can present a typical latent infection. Pigs of any age can form a latent infection after being resistant to acute infection. The virus stays latent in the pig for life. Under certain conditions, the latent virus can be activated, causing recurrent infection and spreading the virus outward. This mechanism makes it difficult to eradicate pseudorabies. Porcine circovirus type 2 (PCV2) is pathogenic and mainly causes a newly discovered infectious disease - multisystemic wasting syndrome in weaned pi...

Claims

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