Propagation method for Asarum forbesiiMaxim tissue culture

A tissue culture and culture medium technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of tuber browning, sub-generation proliferation, lateral bud induction and rooting process of sterile seedlings, etc. The effect of cell division and callus growth promotion

Inactive Publication Date: 2012-05-02
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method can appropriately increase the differentiation rate and proliferation coefficient of callus, the process of subculture proliferation, lateral bud induction and rooting process still needs to be studied.
In addition, there is no report to solve the problem of tuber browning of sterile seedlings in the process of high proliferation rate subculture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1. Select 50 healthy and pest-free Duheng wild seedlings and move them into the greenhouse for potted cultivation, and cut off all the petioles of the plants on the surface. After 60 days, cut the newly germinated aerial parts, remove the leaves, and use the petioles as explants, each about 4-5cm long. A total of 100 petioles were cut.

[0053] 2. Rinse the explants with clean water, scrub the surface with a brush dipped in a little washing powder, then rinse with tap water for 3-5 times, then rinse with "Amway" lotion for 30 minutes, and then rinse with tap water For 30 minutes, 84 disinfectant (diluted 50 times) was sterilized by shaking on a shaker for 10 minutes. After rinsing with running water for 20-30 minutes, sterilize with 75% ethanol for 50-60 seconds on a sterile operating table, immediately pour 0.1% mercuric chloride for 12 minutes, and finally wash 4 times with sterile water, shaking 3-3 times each time. 5 minutes. Cut the sterilized explants into 0.5-...

Embodiment 2

[0061] 1. Select 50 healthy and pest-free Duheng wild seedlings and move them into the greenhouse for potted cultivation, and cut off all the petioles of the plants on the surface. After 60 days, cut the newly germinated aerial part of the plant, remove the leaves, and use the petiole as an explant, each about 4-5cm long. A total of 100 petioles were cut.

[0062] 2. Rinse the explants with clean water, scrub the surface with a brush dipped in a little washing powder, then rinse with tap water for 3-5 times, then rinse with "Amway" lotion for 30 minutes, and then rinse with tap water For 30 minutes, 84 disinfectant (diluted 50 times) was sterilized by shaking on a shaker for 10 minutes. After rinsing with running water for 20-30 minutes, sterilize with 75% ethanol for 50-60 seconds on a sterile operating table, immediately pour 0.1% mercuric chloride for 12 minutes, and finally wash 4 times with sterile water, shaking 3-3 times each time. 5 minutes. Cut the sterilized expla...

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PUM

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Abstract

The invention discloses a propagation method for Asarum forbesiiMaxim tissue culture, comprising the following steps: (1) selecting an explant and sterilizing the explant; (2) inducing and culturing the callus after cutting off the processed explant; (3) subculturing the callus acquired in the step 2; (4) differentiating and inducing the callus processed in the step 3; (5) inducing and culturing the lateral bud of the callus processed in the step 4; and (6) inducing the root of the sprout without root acquired in the step 5. Through the application of the propagation method for Asarum forbesiiMaxim tissue culture, the reproduction rate of the Asarum forbesiiMaxim is increased by 50-72 times so that the reproduction speed is higher.

Description

technical field [0001] The present invention specifically relates to a kind of Du Heng ( Asarum forbesii Maxim .) Propagation methods in tissue culture. Background technique [0002] Du Heng ( Asarum forbesii Maxim .), also known as South Asarum, Aristolochiaceae (Aristolochiaceae) Asarum L. (Asarum L.) perennial herb, tubers with short internodes and many fleshy roots gathered at the lower end. One or two leaves, born at the end of the stem. Single flowers terminal. Capsule fleshy, with numerous dark brown seeds. Growing in damp understory or grass with humus. It is a ground cover plant with great ornamental value in gardens. In addition, the whole plant can be used as medicine, which is one of the precious Chinese medicinal materials. It has the effects of dispelling cold and relieving cough, expelling wind and relieving pain. The main drug ingredients are safrole and a small amount of eugend. Du Heng is not only a good medicinal plant, but also a national sec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 郭忠仁汤诗杰李乃伟宣继萍贾晓东陆小清
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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