Human Crohn disease-simulating murine colitis model, and preparation method and use thereof

A technology for Crohn's disease and colitis, applied in the field of mouse colitis model simulating human Crohn's disease and its preparation

Inactive Publication Date: 2011-06-15
INST OF HEMATOLOGY & BLOOD DISEASES HOSPITAL CHINESE ACADEMY OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although both diseases are abnormal immune responses to antigens in the mucosal system, they result from distinct physiological pathways

Method used

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  • Human Crohn disease-simulating murine colitis model, and preparation method and use thereof
  • Human Crohn disease-simulating murine colitis model, and preparation method and use thereof
  • Human Crohn disease-simulating murine colitis model, and preparation method and use thereof

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Embodiment Construction

[0016] The preparation method of the mouse colitis model simulating human Crohn's disease of the present invention comprises the following steps:

[0017] (1) Inject TNBS into mice by rectal perfusion; (2) Determine whether the mouse model is successfully established by detecting the general signs and clinical indicators of mice; (3) Detect mouse splenocytes by flow cytometry Distribution of T cell subsets to establish models of immune cell changes.

[0018] The general indications of the detected mice refer to: total colonic damage of the mice, weight loss, mental fatigue, loss of luster in coat color, diarrhea and blood in the stool.

[0019] The detection of clinical indicators refers to: intestinal tissue sampling and disease scoring, colon histopathological examination, including membrane congestion and edema, erosion, necrosis, multiple ulcers, deep ulcers, some ulcers reaching the serosa layer, severe necrosis, intestinal lumen Stenosis, the lesion reaches the end of t...

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Abstract

The invention discloses a human Crohn disease-simulating murine colitis model, and a preparation method and use thereof. The preparation method comprises the following steps of: (1) establishing the murine colitis model by using trinitro-benzene-sulfonic acid (TNBS) enema; (2) evaluating the establishment of the model by observing general physical signs and observing the activity status of a mouse in real time; (3) after the mouse is killed, detecting clinical indexes, taking the whole colon of the mouse and performing length measurement, disease scoring and histological examination to evaluate the establishment of the murine colitis model; and (4) taking the splenocyte of the mouse and performing T-cell subset distribution checking to determine the change of immunocytes of the model. The invention provides a support for researches on the detection of clinical colitis mediation, and lays a foundation for development and research of effective treatment medicaments.

Description

technical field [0001] The invention relates to the field of animal models, in particular to a mouse colitis model simulating human Crohn's disease, a preparation method and application thereof. Background technique [0002] Inflammatory bowel disease is a common chronic gastrointestinal disease, including ulcerative colitis and Crohn's colitis, whose etiology and exact pathogenesis are still unclear. At present, it is generally believed that the pathogenesis of IBD is a chronic autoimmune disease mainly manifested by intestinal inflammation due to abnormal immune response of genetically susceptible hosts to intestinal flora due to genetic defects and environmental factors. In recent years, with the improvement of people's living standards, this disease, which is traditionally believed to have a relatively high incidence only in Europe and the United States, is gradually increasing in Asia, and refractory IBD has also increased significantly. In the intestinal tract with in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027A61M31/00
Inventor 韩忠朝梁璐董春兰葛菁陈小军
Owner INST OF HEMATOLOGY & BLOOD DISEASES HOSPITAL CHINESE ACADEMY OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
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